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Standard Name	ftsL
Gene Synonym(s)	ECK0084, b0083, JW0081, yabD, mraR^[1], mraR
Product Desc.	essential cell division protein FtsL^[2]^[3] Cell division and growth, membrane protein^[4] Localizes proteins to septal machinery during cell division. ^[5]
Product Synonyms(s)	membrane bound cell division protein at septum containing leucine zipper motif^[1], B0083^[2]^[1], YabD^[2]^[1], MraR^[2]^[1], FtsL^[2]^[1] , ECK0084, JW0081, mraR, yabD, b0083
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ^[2]
Regulation/Activity
Quick Links
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Notes

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The ftsL gene is essential. FtsL forms a complex with FtsB prior to mid-cell localization; this complex formation requires FtsQ, but not FtsK. Divisome.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ftsL
Mnemonic	Filamentation, temperature sensitive
Synonyms	ECK0084, b0083, JW0081, yabD, mraR^[1], mraR
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	1.96 minutes	MG1655: 91032..91397 <gbrowseImage> name=NC_000913:91032..91397 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 93836..94201 <gbrowseImage> name=NC_012967:93836..94201 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 91031..91396 <gbrowseImage> name=NC_012759:91031..91396 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 91032..91397 <gbrowseImage> name=NC_007779:91032..91397 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 65136..65501 <gbrowseImage> name=NC_010473:65136..65501 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

mraR33

G to A

glu to lys at amino acid 88

Growth Phenotype

thermosensitive lysis and filamentation of rod cells resulting from a defect in cell growth

PMID:1447153

PMID:2676977

mraR33=lts-33

mraR36

Guanine to adenine. in putative ATG start codon

Initial methionine codon changed to isoleucine codon

Growth Phenotype

thermosensitive cell lysis and filamentation resulting from inhibition of cell division; at lower cell density more pronounced thermosensitive growth was observed

PMID:1447153

PMID:2676977

mraR36=fts-36

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0081	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATCAGCAGAGTGACAGAAGC Primer 2:CCTTTTTGCACTACGATATTTTC
6C1	Kohara Phage	Genobase	PMID:3038334
6F3	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[6]	est. P1 cotransduction: 78% ^[7] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[6]	est. P1 cotransduction: 6% ^[7]
pLMG180	Plasmid Clone	ftsL gene cloned pBAD18	PMID:1332942 PMID:7608087
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11086	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11086	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001074	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944803	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1078	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000306	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FtsL
Synonyms	membrane bound cell division protein at septum containing leucine zipper motif^[1], B0083^[2]^[1], YabD^[2]^[1], MraR^[2]^[1], FtsL^[2]^[1] , ECK0084, JW0081, mraR, yabD, b0083
Product description	essential cell division protein FtsL^[2]^[3] Cell division and growth, membrane protein^[4] Localizes proteins to septal machinery during cell division. ^[5]
EC number (for enzymes)
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Notes

Periplasmic domain integrity is essential for FtsL to perform accurately. ^[8]

FtsL forms a ring, similar to FtsZ ring, when it localizes to septum. ^[9]

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007082	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011922	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0131	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007082	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011922	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9906	C	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011922	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0132	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplD	PMID:19402753	LCMS(ID Probability):99.6
Protein	rluA	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source	Notes
plasma membrane		From EcoCyc^[3]
Inner membrane	PMID:1332942	EchoLocation:ftsL	Membrane anchored
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MISRVTEALS KVKGSMGSHE RHALPGVIGD DLLRFGKLPL CLFICIILTA VTVVTTAHHT RLLTAQREQL VLERDALDIE WRNLILEENA LGDHSRVERI ATEKLQMQHV DPSQENIVVQ K
Length	121
Mol. Wt	13.626 kDa
pI	6.9 (calculated)
Extinction coefficient	5,500 - 5,750 (calc based on 0 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	24..120	PF04999 Cell division protein FtsL		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ftsL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128076	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944803	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000306	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEN4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11086	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11086	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944803	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001074	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1078	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli LMG83, LMG145, LMG142 (see table 1)	20 - 40	molecules/cell	Medium: NZY (rich media, recipe described on page 7717) Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	enzyme activity	A chromosomal FtsL-phoA (alkaline phosphatase) fusion was assayed for AP activity & shown to be relatively comparable to the previously reported activity/concentration of FtsQ-phoA estimated at 20-40 molecules per cell (reported by Carson et al., PMID:2007547).	PMID:1332942
Protein	E. coli K-12 MG1655	416	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	201	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	423	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:91012..91052 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0083 (EcoliWiki Page)	NCBI GEO profiles for ftsL
microarray	GenExpDB:b0083 (EcoliWiki Page)	Summary of data for ftsL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ftsL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11086	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1078	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0083
EcoGene	EcoGene:EG11086	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001074	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000306	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FTSL	From SHIGELLACYC
E. coli O157	FTSL	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04999 Cell division protein FtsL
EcoCyc	EcoCyc:EG11086	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11086	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001074	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1078	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000306	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 Blencowe, DK et al. (2011) Identification of a novel function for the FtsL cell division protein from Escherichia coli K12. Biochem. Biophys. Res. Commun. 411 44-9 PubMed
↑ ^6.0 ^6.1 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Ghigo, JM & Beckwith, J (2000) Cell division in Escherichia coli: role of FtsL domains in septal localization, function, and oligomerization. J. Bacteriol. 182 116-29 PubMed
↑ Ghigo, JM et al. (1999) Localization of FtsL to the Escherichia coli septal ring. Mol. Microbiol. 31 725-37 PubMed

ftsL:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ftsL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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