fruB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

fruB

Gene Synonym(s)

ECK2162, b2169, JW2156, fruF, G415[1][2], fpr

Product Desc.

FruB[2][3];

Component of EIIFru[2][3]

Fructosephosphotransferase enzyme III[4]

Product Synonyms(s)

fused fructose-specific PTS enzymes: IIA component[1], HPr component[1], B2169[2][1], FruF[2][1], FruB[2][1] , ECK2162, fpr, fruF, JW2156, b2169

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): fruBKA[2], OP00220, fruBMH

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fruB

Mnemonic

Fructose

Synonyms

ECK2162, b2169, JW2156, fruF, G415[1][2], fpr

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

48.72 minutes 

MG1655: 2261517..2260387
<gbrowseImage> name=NC_000913:2260387..2261517 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2214683..2213553
<gbrowseImage> name=NC_012967:2213553..2214683 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2266829..2265699
<gbrowseImage> name=NC_007779:2265699..2266829 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

fruB(del) (Keio:JW2156)

deletion

deletion

PMID:16738554

Shigen
CGSC9749[5]

fruB725(del)::kan

PMID:16738554

CGSC:101020


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2156

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTTCCAGTTATCCGTACAGGA

Primer 2:CCTGCGCCCTCCCCAAGACCAGC

4F2

Kohara Phage

Genobase

PMID:3038334

12C12

Kohara Phage

Genobase

PMID:3038334

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[5]

est. P1 cotransduction: % [6]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[5]

est. P1 cotransduction: 17% [6]
Synonyms:zeg-722, zei-722::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7990

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10005

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004449

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946677

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0005

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007180

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FruB

Synonyms

fused fructose-specific PTS enzymes: IIA component[1], HPr component[1], B2169[2][1], FruF[2][1], FruB[2][1] , ECK2162, fpr, fruF, JW2156, b2169

Product description

FruB[2][3];

Component of EIIFru[2][3]

Fructosephosphotransferase enzyme III[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016152

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000032

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002114

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005698

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016152

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000032

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002114

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005698

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0598

P

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of EIIFru

could be indirect

Protein

rplE

PMID:16606699

Experiment(s):EBI-1142093

Protein

gcp

PMID:16606699

Experiment(s):EBI-1142093

Protein

katG

PMID:16606699

Experiment(s):EBI-1142093

Protein

rplF

PMID:16606699

Experiment(s):EBI-1142093
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]

Cytoplasm

PMID:8013873, PMID:2841594

EchoLocation:fruB


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MFQLSVQDIH PGEKAGDKEE AIRQVAAALV QAGNVAEGYV NGMLAREQQT STFLGNGIAI
PHGTTDTRDQ VLKTGVQVFQ FPEGVTWGDG QVAYVAIGIA ASSDEHLGLL RQLTHVLSDD
SVAEQLKSAT TAEELRALLM GEKQSEQLKL DNEMLTLDIV ASDLLTLQAL NAARLKEAGA
VDATFVTKAI NEQPLNLGQG IWLSDSAEGN LRSAIAVSRA ANAFDVDGET AAMLVSVAMN
DDQPIAVLKR LADLLLDNKA DRLLKADAAT LLALLTSDDA PTDDVLSAEF VVRNEHGLHA
RPGTMLVNTI KQFNSDITVT NLDGTGKPAN GRSLMKVVAL GVKKGHRLRF TAQGADAEQA
LKAIGDAIAA GLGEGA
Length

376

Mol. Wt

39.647 kDa

pI

4.6 (calculated)

Extinction coefficient

13,980 (calc based on 2 Y, 2 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Modification Site

50

phosphorylation site at T50

probability less than 75%

PMID:17938405

Modification Site

51

phosphorylation site at S51

probability less than 75%

PMID:17938405

Modification Site

52

phosphorylation site at T52

probability less than 75%

PMID:17938405

Modification Site

65

phosphorylation site at T65

probability less than 75%

PMID:17938405

Modification Site

118

phosphorylation site at S118

probability less than 75%

PMID:17938405

Modification Site

64

phosphorylation site at T64

probability greater than 75%

PMID:17938405

Modification Site

5

phosphorylation site at S5

probability greater than 75%

PMID:17938405

Domain

285..371

PF00381 PTS HPr component phosphorylation site

PMID:19920124

Domain

2..142

PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fruB taxon=12017 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130107

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946677

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007180

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P69811

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7990

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10005

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946677

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004449

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0005

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

395

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

184

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

259

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

fruBKA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2261497..2261537 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2169 (EcoliWiki Page)

NCBI GEO profiles for fruB

microarray

GenExpDB:b2169 (EcoliWiki Page)

Summary of data for fruB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2261447..2261907) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ02; Well:B8[7]

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Notes

Accessions Related to fruB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7990

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0005

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2169

EcoGene

EcoGene:EG10005

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004449

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007180

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

FRUB

From SHIGELLACYC

E. coli O157

FRUB

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:55594

Superfamily (EcoliWiki Page)

SUPERFAMILY:55804

Superfamily (EcoliWiki Page)

SUPERFAMILY:55804

Pfam (EcoliWiki Page)

PF00381 PTS HPr component phosphorylation site

Pfam (EcoliWiki Page)

PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

EcoCyc

EcoCyc:G7990

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10005

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004449

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0005

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007180

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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