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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	frc
Gene Synonym(s)	ECK2370, b2374, JW2371, yfdW^[1], yfdW
Product Desc.	formyl-CoA transferase^[2]^[3]; Component of predicted formyl-CoA transferase^[3] Formyl CoA transferase, oxalate catabolism; dimeric^[4]
Product Synonyms(s)	formyl-CoA transferase, NAD(P)-binding^[1], B2374^[2]^[1], YfdW^[2]^[1], Frc^[2]^[1] , ECK2370, JW2371, yfdW, b2374
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): frc^[2]
Regulation/Activity
Quick Links
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Notes

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Orthologous to O. formigenes Frc. EvgAS regulon. The frc-oxc-yfdVE operon is adjacent and convergent on evgAS. Uninduced level is probably low since Sidhu et al. found no activity in E. coli host cells. Two monomers of Frc assemble as interlaced rings, defining the CoA binding site at their interface. Frc binds formylCoA and oxalate. AFMB Structural Genomics ASG target No. 104 (http://afmb.cnrs-mrs.fr/article171.html).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	frc
Mnemonic	FoRmate CoA
Synonyms	ECK2370, b2374, JW2371, yfdW^[1], yfdW
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	53.67 minutes	MG1655: 2491276..2490026 <gbrowseImage> name=NC_000913:2490026..2491276 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 2375831..2377081 <gbrowseImage> name=NC_012759:2375831..2377081 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2498700..2497450 <gbrowseImage> name=NC_007779:2497450..2498700 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2583041..2581791 <gbrowseImage> name=NC_010473:2581791..2583041 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δfrc (Keio:JW2371)	deletion	deletion	PMID:16738554	Shigen CGSC9894^[5]
Δfrc-783::kan			PMID:16738554	CGSC:100990
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2371	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCAACTCCACTTCAAGGAAT Primer 2:CCGATGGCGTGGTTTTGCTTCAT
7G9	Kohara Phage	Genobase	PMID:3038334
10D3	Kohara Phage	Genobase	PMID:3038334
zfb-223::Tn10	Linked marker	CAG18484 = CGSC7406^[5]	est. P1 cotransduction: % ^[6] Synonyms:zej-223::Tn10
zfd-1::Tn10	Linked marker	CAG18467 = CGSC7408^[5]	est. P1 cotransduction: 95% ^[6] Synonyms:zfb-1::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7237	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14145	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003812	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946842	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3897	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007828	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Frc
Synonyms	formyl-CoA transferase, NAD(P)-binding^[1], B2374^[2]^[1], YfdW^[2]^[1], Frc^[2]^[1] , ECK2370, JW2371, yfdW, b2374
Product description	formyl-CoA transferase^[2]^[3]; Component of predicted formyl-CoA transferase^[3] Formyl CoA transferase, oxalate catabolism; dimeric^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003673	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017659	P	Seeded from EcoCyc (v14.0)	complete
	GO:0033608	formyl-CoA transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017659	F	Seeded from EcoCyc (v14.0)	complete
	GO:0033608	formyl-CoA transferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.8.3.16	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of predicted formyl-CoA transferase	could be indirect
Protein	nadE		PMID:16606699	Experiment(s):EBI-1142678
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSTPLQGIKV LDFTGVQSGP SCTQMLAWFG ADVIKIERPG VGDVTRHQLR DIPDIDALYF TMLNSNKRSI ELNTKTAEGK EVMEKLIREA DILVENFHPG AIDHMGFTWE HIQEINPRLI FGSIKGFDEC SPYVNVKAYE NVAQAAGGAA STTGFWDGPP LVSAAALGDS NTGMHLLIGL LAALLHREKT GRGQRVTMSM QDAVLNLCRV KLRDQQRLDK LGYLEEYPQY PNGTFGDAVP RGGNAGGGGQ PGWILKCKGW ETDPNAYIYF TIQEQNWENT CKAIGKPEWI TDPAYSTAHA RQPHIFDIFA EIEKYTVTID KHEAVAYLTQ FDIPCAPVLS MKEISLDPSL RQSGSVVEVE QPLRGKYLTV GCPMKFSAFT PDIKAAPLLG EHTAAVLQEL GYSDDEIAAM KQNHAI
Length	416
Mol. Wt	45.828 kDa
pI	5.4 (calculated)
Extinction coefficient	57,870 - 58,745 (calc based on 13 Y, 7 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	70..244	PF02515 CoA-transferase family III		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=frc taxon=12017 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130306	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946842	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007828	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P69902	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7237	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14145	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946842	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003812	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3897	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	16	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	21	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	13^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
frc
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2491256..2491296 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2374 (EcoliWiki Page)	NCBI GEO profiles for frc
microarray	GenExpDB:b2374 (EcoliWiki Page)	Summary of data for frc from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to frc Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7237	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3897	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2374
EcoGene	EcoGene:EG14145	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003812	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007828	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Bos taurus	ENSBTAP00000029076 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00023664 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00014128 (score: 1.000; bootstrap: 100%) WBGene00016034 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000040100 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000143781 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S2578	From SHIGELLACYC
E. coli O157	Z3639	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02515 CoA-transferase family III
Panther (EcoliWiki Page)	PTHR11837:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:89796
EcoCyc	EcoCyc:G7237	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14145	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003812	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3897	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007828	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

frc:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

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Sequence Features

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Alleles and Phenotypes

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Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to frc Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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