fliM:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fliM

Mnemonic

Flagella

Synonyms

ECK1943, b1945, JW1929, cheC, flaA, QII, cheC2, fla AII, fla QII, flaAII, flaQII, fla, A, AII, flaQ[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

43.5 minutes 

MG1655: 2018111..2019115
<gbrowseImage> name=NC_000913:2018111..2019115 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1910594..1911598
<gbrowseImage> name=NC_012759:1910594..1911598 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2022224..2023228
<gbrowseImage> name=NC_007779:2022224..2023228 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2109119..2110123
<gbrowseImage> name=NC_010473:2109119..2110123 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔfliM (Keio:JW1929)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9605[4]

fliM::Tn5KAN-2 (FB20544)

Insertion at nt 239 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20544

contains pKD46

fliM::Tn5KAN-2 (FB20545)

Insertion at nt 239 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20545

does not contain pKD46

ΔfliM720::kan

PMID:16738554[3]

CGSC:100950


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1929

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGGCGATAGTATTCTTTCTCA

Primer 2:CCTTTGGGCTGTTCCTCGTTCAG

3B3

Kohara Phage

Genobase

PMID:3038334[7]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[4]

est. P1 cotransduction: 35% [8]

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[4]

est. P1 cotransduction: 51% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10323

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10323

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000317

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946442

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0319

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006469

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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