flhE:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

flhE

Mnemonic

Flagella

Synonyms

ECK1879, b1878, JW1867[1], JW1867

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

42.26 minutes, 42.26 minutes 

MG1655: 1960996..1960604
<gbrowseImage> name=NC_000913:1960604..1960996 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1941718..1941350
<gbrowseImage> name=NC_012967:1941350..1941718 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1852663..1853055
<gbrowseImage> name=NC_012759:1852663..1853055 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1964686..1964294
<gbrowseImage> name=NC_007779:1964294..1964686 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2051567..2051175
<gbrowseImage> name=NC_010473:2051175..2051567 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔflhE (Keio:JW1867)

deletion

deletion

PMID:16738554[2]

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1867

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAGAACCTTATTAGCAATATT

Primer 2:CCGCGATAATTCACAATCACTTC

20H4

Kohara Phage

Genobase

PMID:3038334[4]

12C7

Kohara Phage

Genobase

PMID:3038334[4]

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[5]

est. P1 cotransduction: 30% [6]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[5]

est. P1 cotransduction: 31% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7027

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14013

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003614

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946094

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3769

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006270

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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