fldA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fldA

Mnemonic

Flavodoxin

Synonyms

ECK0672, b0684, JW0671[1], JW0671

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

15.31 minutes 

MG1655: 710688..710158
<gbrowseImage> name=NC_000913:710158..710688 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 693560..693030
<gbrowseImage> name=NC_012967:693030..693560 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 612918..613448
<gbrowseImage> name=NC_012759:612918..613448 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 711887..711357
<gbrowseImage> name=NC_007779:711357..711887 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 763280..762750
<gbrowseImage> name=NC_010473:762750..763280 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

710161

Edman degradation

PMID:1999390[2]
PMID:7642583[3]
PMID:7961651[4]
PMID:8076639[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0671

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCTATCACTGGCATCTTTTT

Primer 2:CCGGCATTGAGAATTTCGTCGAG

3A6

Kohara Phage

Genobase

PMID:3038334[7]

11A10

Kohara Phage

Genobase

PMID:3038334[7]

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[8]

est. P1 cotransduction: 72% [9]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[8]

est. P1 cotransduction: 2% [9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10318

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10318

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000312

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945293

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0314

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002332

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Osborne, C et al. (1991) Isolation, cloning, mapping, and nucleotide sequencing of the gene encoding flavodoxin in Escherichia coli. J. Bacteriol. 173 1729-37 PubMed
  3. Birch, OM et al. (1995) Biotin synthase from Escherichia coli, an investigation of the low molecular weight and protein components required for activity in vitro. J. Biol. Chem. 270 19158-65 PubMed
  4. Jenkins, CM & Waterman, MR (1994) Flavodoxin and NADPH-flavodoxin reductase from Escherichia coli support bovine cytochrome P450c17 hydroxylase activities. J. Biol. Chem. 269 27401-8 PubMed
  5. Ifuku, O et al. (1994) Flavodoxin is required for conversion of dethiobiotin to biotin in Escherichia coli. Eur. J. Biochem. 224 173-8 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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