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Gene |

Product(s) |

Expression|

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Standard Name	fhuF
Gene Synonym(s)	ECK4357, b4367, JW4331, yjjS, f262b^[1]^[2]
Product Desc.	acts in reduction of ferrioxamine B iron^[2]^[3] Siderophore-iron reductase; releases iron from hydroxamate-type siderophores; cytoplasmic^[4]
Product Synonyms(s)	ferric iron reductase involved in ferric hydroximate transport ^[1], B4367 ^[2]^[1], YjjS ^[2]^[1], FhuF ^[2]^[1] , ECK4357, JW4331, yjjS, b4367
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fhuF^[2]
Regulation/Activity
Quick Links
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Notes

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CsdB (SufS) and SufD are required to insert an iron-sulfur cluster into FhuF. FhuF is loosely associated with the cytoplasmic membrane. Fur regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fhuF
Mnemonic	Ferric hydroxamate uptake
Synonyms	ECK4357, b4367, JW4331, yjjS, f262b^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	99.21 minutes	MG1655: 4603686..4602898 <gbrowseImage> name=NC_000913:4602898..4603686 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4594897..4594109 <gbrowseImage> name=NC_012967:4594109..4594897 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4541382..4542170 <gbrowseImage> name=NC_012759:4541382..4542170 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4610343..4609555 <gbrowseImage> name=NC_007779:4609555..4610343 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4650148..4649360 <gbrowseImage> name=NC_010473:4649360..4650148 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔfhuF (Keio:JW4331)	deletion	deletion	PMID:16738554	Shigen CGSC11089^[5]
ΔfhuF774::kan			PMID:16738554	CGSC:100858
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4331	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCCTATCGTTCCGCACCGCT Primer 2:CCTTTCAGCGTACAATCGCCACA
8D1	Kohara Phage	Genobase	PMID:3038334
5C1	Kohara Phage	Genobase	PMID:3038334
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[5]	est. P1 cotransduction: 72% ^[6] Synonyms:zjj-202::Tn10, zji-202::Tn10
thrA34::Tn10	Linked marker	CAG18442 = CGSC7320^[5]	est. P1 cotransduction: 22% ^[6] Synonyms:thr-34::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7949	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12595	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004442	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948891	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2480	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014326	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FhuF
Synonyms	ferric iron reductase involved in ferric hydroximate transport ^[1], B4367 ^[2]^[1], YjjS ^[2]^[1], FhuF ^[2]^[1] , ECK4357, JW4331, yjjS, b4367
Product description	acts in reduction of ferrioxamine B iron^[2]^[3] Siderophore-iron reductase; releases iron from hydroxamate-type siderophores; cytoplasmic^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051536	iron-sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0411	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051537	2 iron, 2 sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0001	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	oppD	PMID:16606699	Experiment(s):EBI-1147901
Protein	ydbK	PMID:16606699	Experiment(s):EBI-1147901
Protein	ilvD	PMID:19402753	MALDI(Z-score):27.819555
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAYRSAPLYE DVIWRTHLQP QDPTLAQAVR ATIAKHREHL LEFIRLDEPA PLNAMTLAQW SSPNVLSSLL AVYSDHIYRN QPMMIRENKP LISLWAQWYI GLMVPPLMLA LLTQEKALDV SPEHFHAEFH ETGRVACFWV DVCEDKNATP HSPQHRMETL ISQALVPVVQ ALEATGEING KLIWSNTGYL INWYLTEMKQ LLGEATVESL RHALFFEKTL TNGEDNPLWR TVVLRDGLLV RRTCCQRYRL PDVQQCGDCT LK
Length	262
Mol. Wt	30.112 kDa
pI	6.5 (calculated)
Extinction coefficient	55,920 - 56,670 (calc based on 8 Y, 8 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	93..239	PF06276 Ferric iron reductase FhuF-like transporter	PMID:19920124
Domain	241..262	PF11575 FhuF 2Fe-2S C-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fhuF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132188	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948891	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014326	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39405	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7949	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12595	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948891	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004442	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2480	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	21.407+/-0.272	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.053299492	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	319	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	261	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	138	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fhuF
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4603666..4603706 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4367 (EcoliWiki Page)	NCBI GEO profiles for fhuF
microarray	GenExpDB:b4367 (EcoliWiki Page)	Summary of data for fhuF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4603156..4603694) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:C11^[7]
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Notes

Accessions Related to fhuF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7949	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2480	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4367
EcoGene	EcoGene:EG12595	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004442	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014326	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FHUF	From SHIGELLACYC
E. coli O157	FHUF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF06276 Ferric iron reductase FhuF-like transporter
Pfam (EcoliWiki Page)	PF11575 FhuF 2Fe-2S C-terminal domain
PFAM (EcoliWiki Page)	PFAM:PF06276
EcoCyc	EcoCyc:G7949	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12595	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004442	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2480	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014326	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

fhuF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fhuF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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