eutD:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

eutD

Gene Synonym(s)

eutI, ECK2453, b2458, JW2442, ypfA[1][2], ypfA

Product Desc.

predicted phosphotransacetylase subunit[2][3]

Acetyl phosphotransacylase[4]

Product Synonyms(s)

predicted phosphotransacetylase subunit[1], B2458[2][1], YpfA[2][1], EutD[2][1], EutI[2][1] , ECK2453, eutI, JW2442, ypfA, b2458

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): cchA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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eutD has been characterized in Salmonella.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

eutD

Mnemonic

Ethanolamine utilization

Synonyms

eutI, ECK2453, b2458, JW2442, ypfA[1][2], ypfA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

55.4 minutes 

MG1655: 2571527..2570511
<gbrowseImage> name=NC_000913:2570511..2571527 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2493318..2494334
<gbrowseImage> name=NC_012967:2493318..2494334 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2456316..2457332
<gbrowseImage> name=NC_012759:2456316..2457332 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2572161..2571145
<gbrowseImage> name=NC_007779:2571145..2572161 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

eutI::Tn5KAN-I-SceI (FB20811)

Insertion at nt 542 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20811

does not contain pKD46

eutI::Tn5KAN-I-SceI (FB20812)

Insertion at nt 542 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20812

contains pKD46

ΔeutD778::kan

PMID:16738554

CGSC:106170


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

4E10

Kohara Phage

Genobase

PMID:3038334

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7288

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14188

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003862

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946940

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3940

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008097

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

EutI

Synonyms

predicted phosphotransacetylase subunit[1], B2458[2][1], YpfA[2][1], EutD[2][1], EutI[2][1] , ECK2453, eutI, JW2442, ypfA, b2458

Product description

predicted phosphotransacetylase subunit[2][3]

Acetyl phosphotransacylase[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002505

P

Seeded from EcoCyc (v14.0)

complete

GO:0008415

acyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002505

F

Seeded from EcoCyc (v14.0)

complete

GO:0008415

acyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012147

F

Seeded from EcoCyc (v14.0)

complete

GO:0008415

acyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0012

F

Seeded from EcoCyc (v14.0)

complete

GO:0016407

acetyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004614

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

napC

PMID:16606699

Experiment(s):EBI-1142869

Protein

insF1

PMID:16606699

Experiment(s):EBI-1142869, EBI-1142869, EBI-1142869, EBI-1142869

Protein

nadE

PMID:16606699

Experiment(s):EBI-1142869

Protein

matC

PMID:16606699

Experiment(s):EBI-1142869

Protein

ubiB

PMID:16606699

Experiment(s):EBI-1142869

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MIIERCRELA LRAPARVVFP DALDQRVLKA AQYLHQQGLA TPILVANPFE LRQFALSHGV
AMDGLQVIDP HGNLAMREEF AHRWLARAGE KTPPDALEKL TDPLMFAAAM VSAGKADVCI
AGNLSSTANV LRAGLRIIGL QPGCKTLSSI FLMLPQYSGP ALGFADCSVV PQPTAAQLAD
IALASAETWR AITGEEPRVA MLSFSSNGSA RHPCVANVQQ ATEIVRERAP KLVVDGELQF
DAAFVPEVAA QKAPASPLQG KANVMVFPSL EAGNIGYKIA QRLGGYRAVG PLIQGLAAPM
HDLSRGCSVQ EIIELALVAA VPRQTEVNRE SSLQTLVE
Length

338

Mol. Wt

36.066 kDa

pI

6.7 (calculated)

Extinction coefficient

16,960 - 17,710 (calc based on 4 Y, 2 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..320

PF01515 Phosphate acetyl/butaryl transferase

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=eutD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130383

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946940

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008097

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77218

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7288

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14188

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946940

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003862

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3940

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

0.168+/-0.03

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

cchA

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2571507..2571547 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2458 (EcoliWiki Page)

NCBI GEO profiles for eutD

microarray

GenExpDB:b2458 (EcoliWiki Page)

Summary of data for eutD from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to eutD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7288

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3940

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2458

EcoGene

EcoGene:EG14188

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003862

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008097

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Panther (EcoliWiki Page)

PTHR23406:SF3

Superfamily (EcoliWiki Page)

SUPERFAMILY:53659

Pfam (EcoliWiki Page)

PF01515 Phosphate acetyl/butaryl transferase

EcoCyc

EcoCyc:G7288

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14188

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003862

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3940

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008097

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

Categories

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