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Standard Name	emrA
Gene Synonym(s)	ECK2679, b2685, JW2660^[1], JW2660
Product Desc.	EmrA Membrane Fusion Protein^[2]^[3]; Component of EmrAB-TolC Multidrug Efflux Transport System^[3] EmrAB-TolC multidrug resistance pump; periplasmic membrane fusion component, signal anchor; energy-dependent efflux of octane, thiolactomycin and the uncouplers DNP, CCCP, and FCCP^[4]
Product Synonyms(s)	multidrug efflux system^[1], B2685^[2]^[1], EmrA^[2]^[1] , ECK2679, JW2660, b2685
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): emrRAB^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	emrA
Mnemonic	Efflux-multidrug resistance
Synonyms	ECK2679, b2685, JW2660^[1], JW2660
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.55 minutes	MG1655: 2809449..2810621 <gbrowseImage> name=NC_000913:2809449..2810621 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2706286..2707458 <gbrowseImage> name=NC_012967:2706286..2707458 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2695261..2696433 <gbrowseImage> name=NC_012759:2695261..2696433 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2810083..2811255 <gbrowseImage> name=NC_007779:2810083..2811255 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2901991..2903163 <gbrowseImage> name=NC_010473:2901991..2903163 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔemrA (Keio:JW2660)	deletion	deletion			PMID:16738554	Shigen CGSC10098^[5]
ΔemrA766::kan					PMID:16738554	CGSC:100698
pEMR2.1 in strain RP437			Resistant to	This milticopy plasmid in strain RP437, emrA ⁺ & emrB ⁺ in pUC18, is resistant to Nalidixic acid	PMID:1409590	Parental: RP437 CGSC Expiramental: RP437 with plasmid pEMR2.1
pEMR2.1 in strain RP437			Resistant to	This milticopy plasmid in strain RP437, emrA ⁺ & emrB ⁺ in pUC18, is resistant to Carbonyl cyanide m-chlorophenyl hydrazone (CCCP).	PMID:1409590	Parental: RP437 CGSC Expiramental: RP437 with plasmid pEMR2.1
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Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2660	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCGCAAATGCGGAGACTCA Primer 2:CCGCCAGCGTTAGCTTTTACGAT
8G10	Kohara Phage	Genobase	PMID:3038334
23B7	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: 1% ^[6]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 56% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11354	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11354	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001323	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947166	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1329	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008838	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	EmrA
Synonyms	multidrug efflux system^[1], B2685^[2]^[1], EmrA^[2]^[1] , ECK2679, JW2660, b2685
Product description	EmrA Membrane Fusion Protein^[2]^[3]; Component of EmrAB-TolC Multidrug Efflux Transport System^[3] EmrAB-TolC multidrug resistance pump; periplasmic membrane fusion component, signal anchor; energy-dependent efflux of octane, thiolactomycin and the uncouplers DNP, CCCP, and FCCP^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006855	drug transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005694	P	Seeded from EcoCyc (v14.0)	complete
	GO:0031226	intrinsic to plasma membrane	PMID:1409590	IMP: Inferred from Mutant Phenotype		C		complete
	GO:0008565	protein transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009276	Gram-negative-bacterium-type cell wall	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005694	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009306	protein secretion	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015238	drug transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005694	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9904	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of EmrAB-TolC Multidrug Efflux Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSANAETQTP QQPVKKSGKR KRLLLLLTLL FIIIAVAIGI YWFLVLRHFE ETDDAYVAGN QIQIMSQVSG SVTKVWADNT DFVKEGDVLV TLDPTDARQA FEKAKTALAS SVRQTHQLMI NSKQLQANIE VQKIALAKAQ SDYNRRVPLG NANLIGREEL QHARDAVTSA QAQLDVAIQQ YNANQAMILG TKLEDQPAVQ QAATEVRNAW LALERTRIIS PMTGYVSRRA VQPGAQISPT TPLMAVVPAT NMWVDANFKE TQIANMRIGQ PVTITTDIYG DDVKYTGKVV GLDMGTGSAF SLLPAQNATG NWIKVVQRLP VRIELDQKQL EQYPLRIGLS TLVSVNTTNR DGQVLANKVR STPVAVSTAR EISLAPVNKL IDDIVKANAG
Length	390
Mol. Wt	42.735 kDa
pI	9.8 (calculated)
Extinction coefficient	39,420 (calc based on 8 Y, 5 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	60..343	PF00529 HlyD family secretion protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=emrA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130597	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947166	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008838	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P27303	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11354	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11354	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947166	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001323	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1329	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.79E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	770	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	229	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	317	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
emrRAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2809429..2809469 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2685 (EcoliWiki Page)	NCBI GEO profiles for emrA
microarray	GenExpDB:b2685 (EcoliWiki Page)	Summary of data for emrA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2809252..2809498) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ13; Well:G2^[7]
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Notes

Accessions Related to emrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11354	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1329	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2685
EcoGene	EcoGene:EG11354	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001323	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008838	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Macaca mulatta	ENSMMUP00000003055 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000051763 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	EMRA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00529 HlyD family secretion protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:111369
EcoCyc	EcoCyc:EG11354	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11354	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001323	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1329	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008838	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

emrA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to emrA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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