dnaE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
dnaE |
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| Mnemonic |
DNA |
| Synonyms |
ECK0183, b0184, JW0179, polC, sdgC[1], sdgC |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
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MG1655 |
4.42 minutes |
MG1655: 205126..208608 |
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NC_012967: 207968..211450 |
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NC_012759: 205125..208607 |
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W3110 |
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W3110: 205126..208608 |
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DH10B: 179230..182712 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
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dnaE1026(ts) |
temperature sensitive |
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dnaE486(ts) |
temperature sensitive |
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dnaE1044 |
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dnaE1061 |
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dnaE1126 |
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dnaE74(ts) |
temperature sensitive |
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dnaE786 (araD-polB)(del)::omega |
Deletion of araD-polB operon and replaced with omega cassette. Fusion with rapid stop allele dnaE846 |
Sensitivity to |
There is a significant delay in the resumption of DNA replication after exposure to UV radiation at 30 C |
See figure 4 B. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0179 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCTGAACCACGTTTCGTACA Primer 2:CCGTCAAACTCCAGTTCCACCTG | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 6% [8] | ||
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Linked marker |
est. P1 cotransduction: 36% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Brotcorne-Lannoye, A et al. (1985) Involvement of DNA polymerase III in UV-induced mutagenesis of bacteriophage lambda. Mol. Gen. Genet. 199 64-9 PubMed
- ↑ Strauss, B et al. (2004) Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene. J. Bacteriol. 186 2147-55 PubMed
- ↑ Rangarajan, S et al. (1999) A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 96 9224-9 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
