PMID:3158798
| Citation |
Brotcorne-Lannoye, A, Maenhaut-Michel, G and Radman, M (1985) Involvement of DNA polymerase III in UV-induced mutagenesis of bacteriophage lambda. Mol. Gen. Genet. 199:64-9 |
|---|---|
| Abstract |
It has been proposed that the mutation fixation processes stimulated by SOS induction result from an induced infidelity of DNA replication (Radman 1974). The aim of this study was to determine if mutator mutations in the E. coli DNA polymerase III might affect UV-induced mutagenesis. Using a phage lambda mutation assay which can discriminate between targeted and untargeted mutations, we show that the polC74 mutator mutation (Sevastopoulos and Glaser 1977) primarily affects untargeted mutagenesis, which occurs in a recA1 genetic background and is amplified in the recA+ genetic background. The polC74 mutation also increases the UV-induced mutagenesis of the bacterial chromosome. These results suggest that DNA polymerase III is involved in the process of UV-induced mutagenesis in E. coli. |
| Links | |
| Keywords |
Bacteriophage lambda/genetics; Bacteriophage lambda/radiation effects; DNA Polymerase III/genetics; DNA, Bacterial/genetics; DNA, Viral/genetics; DNA-Directed DNA Polymerase/genetics; Escherichia coli/genetics; Escherichia coli/radiation effects; Mutation/radiation effects; Ultraviolet Rays |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
