degQ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

degQ

Mnemonic

Degradative protease

Synonyms

ECK3223, b3234, JW3203, hhoA[1], hhoA

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.82 minutes 

MG1655: 3378765..3380132
<gbrowseImage> name=NC_000913:3378765..3380132 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3308641..3310008
<gbrowseImage> name=NC_012967:3308641..3310008 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3265913..3267280
<gbrowseImage> name=NC_012759:3265913..3267280 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3380598..3381965
<gbrowseImage> name=NC_007779:3380598..3381965 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3476510..3477877
<gbrowseImage> name=NC_010473:3476510..3477877 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3378846

Edman degradation

PMID:8576051[2]
PMID:8576052[3]
PMID:9740056[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdegQ (Keio:JW3203)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC10429[6]

degQ::Tn5KAN-I-SceI (FB21077)

Insertion at nt 833 in Minus orientation

PMID:15262929[7]

E. coli Genome Project:FB21077

contains pKD46

degQ::Tn5KAN-I-SceI (FB21078)

Insertion at nt 833 in Minus orientation

PMID:15262929[7]

E. coli Genome Project:FB21078

does not contain pKD46

ΔdegQ760::kan

PMID:16738554[5]

CGSC:100572


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3203

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAAAAACAAACCCAGCTGTT

Primer 2:CCACGCATCAGCAGATAGATGCT

1E3

Kohara Phage

Genobase

PMID:3038334[9]

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[6]

est. P1 cotransduction: 59% [10]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[6]

est. P1 cotransduction: 23% [10]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7682

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12612

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004229

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947812

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2496

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010609

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Waller, PR & Sauer, RT (1996) Characterization of degQ and degS, Escherichia coli genes encoding homologs of the DegP protease. J. Bacteriol. 178 1146-53 PubMed
  3. Bass, S et al. (1996) Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and a truncated R1pA. J. Bacteriol. 178 1154-61 PubMed
  4. Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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