def:Gene

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

def

Mnemonic

Deformylase

Synonyms

ECK3273, b3287, JW3248, fms[1], fms

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

73.96 minutes 

MG1655: 3431712..3432221
<gbrowseImage> name=NC_000913:3431712..3432221 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3361595..3362104
<gbrowseImage> name=NC_012967:3361595..3362104 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3318869..3319378
<gbrowseImage> name=NC_012759:3318869..3319378 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4206726..4206217
<gbrowseImage> name=NC_007779:4206217..4206726 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3529457..3529966
<gbrowseImage> name=NC_010473:3529457..3529966 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3431715

Edman degradation

PMID:8244948[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3248

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCTCAGTTTTGCAAGTGTTACA

Primer 2:CCAGCCCGGGCTTTCAGACGATC

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[4]

est. P1 cotransduction: 91% [5]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 1% [5]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

JM101Tr/ pUCdef

Plasmid Clone

PMID:8244948[2]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11440

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11440

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001404

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947780

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1410

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010779

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Meinnel, T & Blanquet, S (1993) Evidence that peptide deformylase and methionyl-tRNA(fMet) formyltransferase are encoded within the same operon in Escherichia coli. J. Bacteriol. 175 7737-40 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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