dctA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
dctA |
|---|---|
| Mnemonic |
Dicarboxylate transport |
| Synonyms |
ECK3513, b3528, JW3496[1], JW3496, out |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
79.32 minutes |
MG1655: 3681470..3680184 |
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NC_012967: 3612939..3611653 |
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NC_012759: 3568678..3569964 |
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W3110 |
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W3110: 3956968..3958254 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Strain DH10B |
M311I |
Nonsynonomous mutation |
This is a SNP in E. coli K-12 Strain DH10B | ||||
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ΔdctA (Keio:JW3496) |
deletion |
deletion |
Auxotrophies |
Cannot utilize fumurate or malate as the sole carbon source (PMID:10482502[3]). Cannot utilize orotate for pyrimidine biosynthesis (PMID:8955389[4]). |
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dctA::Tn5KAN-I-SceI (FB21256) |
Insertion at nt 377 in Plus orientation |
contains pKD46 | |||||
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dctA2 |
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dctA4 |
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dctA5 |
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ΔdctA783::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3496 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAACCTCTCTGTTTAAAAG Primer 2:CCAGAGGATAATTCGTGCGTTTT | |
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Kohara Phage |
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Kohara Phage |
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zhg-50::Tn10 |
Linked marker |
est. P1 cotransduction: % [12] | |
|
zic-4901::Tn10 |
Linked marker |
est. P1 cotransduction: % [12] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
- ↑ Davies, SJ et al. (1999) Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli. J. Bacteriol. 181 5624-35 PubMed
- ↑ Baker, KE et al. (1996) Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA. J. Bacteriol. 178 7099-105 PubMed
- ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Lo, TC & Sanwal, BD (1975) Genetic analysis of mutants of Escherichia coli defective in dicarboxylate transport. Mol. Gen. Genet. 140 303-7 PubMed
- ↑ Lo, TC et al. (1972) Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. J. Biol. Chem. 247 6323-31 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
