cydA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cydA

Mnemonic

Cytochrome d

Synonyms

ECK0721, b0733, JW0722, cyd-1[1]

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

16.61 minutes 

MG1655: 770681..772249
<gbrowseImage> name=NC_000913:770681..772249 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 752340..753908
<gbrowseImage> name=NC_012967:752340..753908 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 673441..675009
<gbrowseImage> name=NC_012759:673441..675009 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 771880..773448
<gbrowseImage> name=NC_007779:771880..773448 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 823273..824841
<gbrowseImage> name=NC_010473:823273..824841 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

770681

Edman degradation

PMID:10428033[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

cydA::Tn5KAN-2 (FB20228)

Insertion at nt 568 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB20228

does not contain pKD46

cydA85

PMID:15028700[4]

CGSC:85756

cydA2

CGSC:10372

CydAB (del), cyoABCD (del), cbdAB (del), ygiN (del)

deletion

deletion

respiration inhibitor

deletion of all genes decreased oxygen uptake by 98% via activation of arcA, table 1.

PMID:20709841[5]

CydAB(del), cyoABCD(del), cbdAB(del), ygiN(del)

deletion

deletion

Growth Phenotype

Mutaiton causes decreased growth rate via inhibition of arcA, table 1.

PMID:20709841[5]

CydAB(del), cyoABCD(del), cbdAB(del), ygiN(del)

deletion

deletion

  • Increase in glucose uptake rate
  • increase in lactate secretion rate
  • Decrease in acetate secretion rate

PMID:20709841[5]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0722

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTTAGATATAGTCGAACTGTC

Primer 2:CCGCGTGCCGGCTGAGTAGTCGT

18F11

Kohara Phage

Genobase

PMID:3038334[7]

10G5

Kohara Phage

Genobase

PMID:3038334[7]

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[8]

est. P1 cotransduction: 1% [9]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[8]

est. P1 cotransduction: 74% [9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10173

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10173

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000168

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945341

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0170

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002499

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Bader, M et al. (1999) Oxidative protein folding is driven by the electron transport system. Cell 98 217-27 PubMed
  3. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  4. Strauss, B et al. (2004) Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene. J. Bacteriol. 186 2147-55 PubMed
  5. 5.0 5.1 5.2 Portnoy, VA et al. (2010) Deletion of genes encoding cytochrome oxidases and quinol monooxygenase blocks the aerobic-anaerobic shift in Escherichia coli K-12 MG1655. Appl. Environ. Microbiol. 76 6529-40 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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