cutC:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cutC

Mnemonic

Cu tolerance

Synonyms

ECK1875, b1874, JW1863, dinY?[1][2], dinY?

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

 

MG1655: 1957290..1956544
<gbrowseImage> name=NC_000913:1956544..1957290 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1937730..1937290
<gbrowseImage> name=NC_012967:1937290..1937730 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1848603..1849349
<gbrowseImage> name=NC_012759:1848603..1849349 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1960980..1960234
<gbrowseImage> name=NC_007779:1960234..1960980 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

42.17 minutes 

MG1655: 1956984..1956544
<gbrowseImage> name=NC_000913:1956544..1956984 source=ecoli preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2047861..2047115
<gbrowseImage> name=NC_010473:2047115..2047861 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcutC (Keio:JW1863)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9553[4]

ΔcutC728::kan

PMID:16738554[3]

CGSC:100452

cutC
(Strain: GME111)

bp 146 (from A to G) in cutC
Mutations in cutF also: bp 32 of (from C to T) & bp 301 (from A to T)

substitution of Lys-49 by Arg-49 in cutC
AND: first mutation in cutF: Ala-10 to Val-10 (in signal peptide) & second mutation in cutF (in mature protein): Thr-81 by Ser-81

Sensitivity to

Copper
Additionally, resistant to P1 infection

PMID:7635807[5]

Note that this cutC strain contains additional mutations in cutF.

<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1863

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCATTACTGGAAATTTGCTG

Primer 2:CCTTTGGCCTGATGGCGTTCAAT

20H4

Kohara Phage

Genobase

PMID:3038334[7]

12C7

Kohara Phage

Genobase

PMID:3038334[7]

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[4]

est. P1 cotransduction: % [8]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 27% [8]
Synonyms:car-96::Tn10

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7024

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12956

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003611

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:2847756

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2790

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006252

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Gupta, SD et al. (1995) Identification of cutC and cutF (nlpE) genes involved in copper tolerance in Escherichia coli. J. Bacteriol. 177 4207-15 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories