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Standard Name	cutA
Gene Synonym(s)	ECK4131, b4137, JW4097, cutA1, cycY^[1], cycY
Product Desc.	copper binding protein CutA^[2]^[3]; Component of copper binding protein CutA^[3] Copper sensitivity, binds Cu(II); possible role in cytochrome c maturation; trimeric^[4]
Product Synonyms(s)	copper binding protein, copper sensitivity^[1], B4137^[2]^[1], CutA^[2]^[1] , cutA1, cycY, ECK4131, JW4097, b4137
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cutA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cutA
Mnemonic	Cu tolerance
Synonyms	ECK4131, b4137, JW4097, cutA1, cycY^[1], cycY
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	94.04 minutes	MG1655: 4363379..4363041 <gbrowseImage> name=NC_000913:4363041..4363379 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4344077..4343739 <gbrowseImage> name=NC_012967:4343739..4344077 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4301776..4302114 <gbrowseImage> name=NC_012759:4301776..4302114 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4370034..4369696 <gbrowseImage> name=NC_007779:4369696..4370034 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4463741..4463403 <gbrowseImage> name=NC_010473:4463403..4463741 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔcutA (Keio:JW4097)	deletion	deletion	PMID:16738554	Shigen CGSC10951^[5]
ΔcutA763::kan			PMID:16738554	CGSC:100450
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4097	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTTGATGAAAAAAGTTCGAA Primer 2:CCGCGTAAAGATGCGTTGAGCCA
5G7	Kohara Phage	Genobase	PMID:3038334
9B1	Kohara Phage	Genobase	PMID:3038334
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 81% ^[6] Synonyms:zjd-2231::Tn10
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 3% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12177	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12177	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002070	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948660	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2094	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013544	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CutA
Synonyms	copper binding protein, copper sensitivity^[1], B4137^[2]^[1], CutA^[2]^[1] , cutA1, cycY, ECK4131, JW4097, b4137
Product description	copper binding protein CutA^[2]^[3]; Component of copper binding protein CutA^[3] Copper sensitivity, binds Cu(II); possible role in cytochrome c maturation; trimeric^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005507	copper ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0186	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01160	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0010038	response to metal ion	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004323	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of copper binding protein CutA	could be indirect
Protein	yegL		PMID:15690043	Experiment(s):EBI-880959
Protein	tufA		PMID:15690043	Experiment(s):EBI-880959
Protein	glgS		PMID:16606699	Experiment(s):EBI-1147520
Protein	rpsC		PMID:16606699	Experiment(s):EBI-1147520
Protein	rpsB		PMID:16606699	Experiment(s):EBI-1147520
Protein	nadE		PMID:16606699	Experiment(s):EBI-1147520
Protein	rplB		PMID:16606699	Experiment(s):EBI-1147520
Protein	tufB		PMID:19402753	MALDI(Z-score):21.029334
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLDEKSSNTA SVVVLCTAPD EATAQDLAAK VLAEKLAACA TLIPGATSLY YWEGKLEQEY EVQMILKTTV SHQQALLECL KSHHPYQTPE LLVLPVTHGD TDYLSWLNAS LR
Length	112
Mol. Wt	12.33 kDa
pI	4.8 (calculated)
Extinction coefficient	18,450 - 18,825 (calc based on 5 Y, 2 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	11..112	PF03091 CutA1 divalent ion tolerance protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cutA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131962	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948660	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013544	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P69488	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12177	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12177	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948660	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002070	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2094	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	156	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	207	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	177^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cutA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4363359..4363399 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4137 (EcoliWiki Page)	NCBI GEO profiles for cutA
microarray	GenExpDB:b4137 (EcoliWiki Page)	Summary of data for cutA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4362845..4363113) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ11; Well:C9^[7]
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Notes

Accessions Related to cutA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12177	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2094	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4137
EcoGene	EcoGene:EG12177	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002070	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013544	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G33740 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000002142 (score: 1.000; bootstrap: 100%) ENSBTAP00000022554 (score: 0.125)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00037532 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00009443 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000001427 (score: 1.000; bootstrap: 100%) ENSCAFP00000005315 (score: 0.166)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040426-1320 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-2254 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0030545 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000230276 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000013966 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000024493 (score: 1.000; bootstrap: 100%) ENSMODP00000011485 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1914925 (score: 1.000; bootstrap: 100%) MGI:1925246 (score: 0.092)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000030825 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000000568 (score: 1.000; bootstrap: 100%) ENSRNOP00000025501 (score: 0.146)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000159894 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00034816001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000042375 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	CUTA	From SHIGELLACYC
E. coli O157	CUTA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03091 CutA1 divalent ion tolerance protein
Panther (EcoliWiki Page)	PTHR23419:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:54913
EcoCyc	EcoCyc:EG12177	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12177	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002070	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2094	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013544	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cutA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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