cpdB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cpdB

Mnemonic

Cyclic nucleotide P-diesterase

Synonyms

ECK4209, b4213, JW4171[1], JW4171

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

95.54 minutes, 95.54 minutes 

MG1655: 4434588..4432645
<gbrowseImage> name=NC_000913:4432645..4434588 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4421559..4419616
<gbrowseImage> name=NC_012967:4419616..4421559 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4371380..4373323
<gbrowseImage> name=NC_012759:4371380..4373323 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4441245..4439302
<gbrowseImage> name=NC_007779:4439302..4441245 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4534950..4533007
<gbrowseImage> name=NC_010473:4533007..4534950 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4432648

Edman degradation

PMID:9298646[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcpdB (Keio:JW4171)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC11004[4]

cpdB1

CGSC:11200

cpdB2

CGSC:11205

ΔcpdB762::kan

PMID:16738554[3]

CGSC:100380


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4171

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCATTAAGTTTAGCGCAACGCT

Primer 2:CCtTTACTCAAATCCACCTGATA

7E9

Kohara Phage

Genobase

PMID:3038334[6]

5B5

Kohara Phage

Genobase

PMID:3038334[6]

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[4]

est. P1 cotransduction: 81% [7]

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[4]

est. P1 cotransduction: 66% [7]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10160

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10160

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000156

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948729

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0158

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013782

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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