chbC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

chbC

Gene Synonym(s)

ECK1735, b1737, JW1726, celB, hic[1]

Product Desc.

ChbC[2][3];

Component of EIIChb[2][3]

PTS Enzyme IIC(Chb) for N,N'-diacetylchitobiose utilization[4]

Product Synonyms(s)

N,N'-diacetylchitobiose-specific enzyme IIC component of PTS[1], B1737[2][1], ChbC[2][1], Hic[2][1], CelB[2][1] , celB, ECK1735, JW1726, b1737

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): chbBCARFG[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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The chb operon is induced by N,N'-diacetylchitobiose. The chb operon was previously thought to encode a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent, but a fully induced operon did not support growth on cellobiose, implying a mutation altering the substrate specificity is needed; several candidate mutations were noted in Genbank, but not proven to alter the specificity of the system (Keyhani, 1997). In contrast, a later study claim that mutations making the operon expression high-level constitutive by chbR and nagC mutations are sufficient for the Cel+ phenotype and that no mutations altering substrate specificity are required (Kachroo, 2007).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

chbC

Mnemonic

Chitobiose

Synonyms

ECK1735, b1737, JW1726, celB, hic[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

39.18 minutes 

MG1655: 1819238..1817880
<gbrowseImage> name=NC_000913:1817880..1819238 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1797121..1798479
<gbrowseImage> name=NC_012967:1797121..1798479 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1709939..1711297
<gbrowseImage> name=NC_012759:1709939..1711297 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1822928..1821570
<gbrowseImage> name=NC_007779:1821570..1822928 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1909809..1908451
<gbrowseImage> name=NC_010473:1908451..1909809 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔchbC (Keio:JW1726)

deletion

deletion

PMID:16738554

Shigen
CGSC9458[5]

ΔchbC736::kan

PMID:16738554

CGSC:100288


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1726

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTAATGTTATTGCATCGCT

Primer 2:CCAAATTTCAGGGCGTTAGCGAT

19B6

Kohara Phage

Genobase

PMID:3038334

4F7

Kohara Phage

Genobase

PMID:3038334

5E12

Kohara Phage

Genobase

PMID:3038334

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 18% [6]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[5]

est. P1 cotransduction: 59% [6]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10141

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10141

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000137

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945982

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0139

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005795

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ChbC

Synonyms

N,N'-diacetylchitobiose-specific enzyme IIC component of PTS[1], B1737[2][1], ChbC[2][1], Hic[2][1], CelB[2][1] , celB, ECK1735, JW1726, b1737

Product description

ChbC[2][3];

Component of EIIChb[2][3]

PTS Enzyme IIC(Chb) for N,N'-diacetylchitobiose utilization[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004501

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004796

F

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004501

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004796

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0598

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004501

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004796

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of EIIChb

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 10 predicted transmembrane domains

Daley et al. (2005) [7]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSNVIASLEK VLLPFAVKIG KQPHVNAIKN GFIRLMPLTL AGAMFVLINN VFLSFGEGSF
FYSLGIRLDA STIETLNGLK GIGGNVYNGT LGIMSLMAPF FIGMALAEER KVDALAAGLL
SVAAFMTVTP YSVGEAYAVG ANWLGGANII SGIIIGLVVA EMFTFIVRRN WVIKLPDSVP
ASVSRSFSAL IPGFIILSVM GIIAWALNTW GTNFHQIIMD TISTPLASLG SVVGWAYVIF
VPLLWFFGIH GALALTALDN GIMTPWALEN IATYQQYGSV EAALAAGKTF HIWAKPMLDS
FIFLGGSGAT LGLILAIFIA SRRADYRQVA KLALPSGIFQ INEPILFGLP IIMNPVMFIP
FVLVQPILAA ITLAAYYMGI IPPVTNIAPW TMPTGLGAFF NTNGSVAALL VALFNLGIAT
LIYLPFVVVA NKAQNAIDKE ESEEDIANAL KF
Length

452

Mol. Wt

48.334 kDa

pI

7.0 (calculated)

Extinction coefficient

65,890 (calc based on 11 Y, 9 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

29..364

PF02378 Phosphotransferase system, EIIC

PMID:19920124

<motif_map/>

tmhmm.php?gene=chbC&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=chbC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129691

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945982

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005795

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P17334

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10141

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10141

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945982

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000137

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0139

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

14

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

3

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

chbBCARFG

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1819218..1819258 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1737 (EcoliWiki Page)

NCBI GEO profiles for chbC

microarray

GenExpDB:b1737 (EcoliWiki Page)

Summary of data for chbC from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to chbC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10141

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0139

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1737

EcoGene

EcoGene:EG10141

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000137

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005795

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000033563 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

E. coli O157

CELB

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02378 Phosphotransferase system, EIIC

EcoCyc

EcoCyc:EG10141

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10141

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000137

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0139

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005795

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

Categories

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