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Standard Name	chbA
Gene Synonym(s)	ECK1734, b1736, JW1725, celC^[1], celC
Product Desc.	ChbA^[2]^[3]; Component of EIIChb^[2]^[3] PTS Enzyme IIA(Chb), N,N'-diacetylchitobiose utilization; trimeric^[4]
Product Synonyms(s)	N,N'-diacetylchitobiose-specific enzyme IIA component of PTS^[1], B1736^[2]^[1], ChbA^[2]^[1], CelC^[2]^[1] , celC, ECK1734, JW1725, b1736
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): chbBCARFG^[2]
Regulation/Activity
Quick Links
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Notes

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The chb operon is induced by N,N'-diacetylchitobiose. The chb operon was previously thought to encode a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent, but a fully induced operon did not support growth on cellobiose, implying a mutation altering the substrate specificity is needed; several candidate mutations were noted in Genbank, but not proven to alter the specificity of the system (Keyhani, 1997). In contrast, a later study claim that mutations making the operon expression high-level constitutive by chbR and nagC mutations are sufficient for the Cel+ phenotype and that no mutations altering substrate specificity are required (Kachroo, 2007).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	chbA
Mnemonic	Chitobiose
Synonyms	ECK1734, b1736, JW1725, celC^[1], celC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	39.17 minutes	MG1655: 1817829..1817479 <gbrowseImage> name=NC_000913:1817479..1817829 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1709538..1709888 <gbrowseImage> name=NC_012759:1709538..1709888 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1821519..1821169 <gbrowseImage> name=NC_007779:1821169..1821519 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1908400..1908050 <gbrowseImage> name=NC_010473:1908050..1908400 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔchbA (Keio:JW1725)	deletion	deletion		PMID:16738554	Shigen CGSC9457^[5]
chbAM59I		M59I	(in strain: ECOR 50)			Strain variation; seeded from UniProt:P69791
chbAA14V		A14V	(in strain: ECOR 1)			Strain variation; seeded from UniProt:P69791
chbAA52T		A52T	(in strain: ECOR 61)			Strain variation; seeded from UniProt:P69791
ΔchbA735::kan				PMID:16738554	CGSC:100284
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1725	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGGATCTCGATAACATTCC Primer 2:CCTGCCTTCAGTTTTTCATGAAG
19B6	Kohara Phage	Genobase	PMID:3038334
4F7	Kohara Phage	Genobase	PMID:3038334
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 18% ^[6] Synonyms:zdh-925::Tn10
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 58% ^[6] Synonyms:zdi-276::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10142	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10142	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000138	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946244	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0140	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005792	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ChbA
Synonyms	N,N'-diacetylchitobiose-specific enzyme IIA component of PTS^[1], B1736^[2]^[1], ChbA^[2]^[1], CelC^[2]^[1] , celC, ECK1734, JW1725, b1736
Product description	ChbA^[2]^[3]; Component of EIIChb^[2]^[3] PTS Enzyme IIA(Chb), N,N'-diacetylchitobiose utilization; trimeric^[4]
EC number (for enzymes)	2.7.1.69^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005351	sugar:hydrogen symporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003188	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003188	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008643	carbohydrate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003188	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0598	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003188	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of EIIChb	could be indirect
Protein	rplE		PMID:16606699	Experiment(s):EBI-1140650
Protein	insB2		PMID:16606699	Experiment(s):EBI-1140650, EBI-1140650
Protein	yjgZ		PMID:16606699	Experiment(s):EBI-1140650
Protein	nfnB		PMID:16606699	Experiment(s):EBI-1140650
Protein	crcB		PMID:16606699	Experiment(s):EBI-1140650
Protein	yafQ		PMID:16606699	Experiment(s):EBI-1140650
Protein	ycgZ		PMID:16606699	Experiment(s):EBI-1140650
Protein	yhdN		PMID:16606699	Experiment(s):EBI-1140650
Protein	rpsT		PMID:16606699	Experiment(s):EBI-1140650
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA KAMMDQSRMA LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA RELITELIEL HEKLKA
Length	116
Mol. Wt	12.747 kDa
pI	4.7 (calculated)
Extinction coefficient	1,490 (calc based on 1 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	18..113	PF02255 PTS system, Lactose/Cellobiose specific IIA subunit		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=chbA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129690	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946244	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005792	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P69791	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10142	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10142	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946244	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000138	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0140	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	99	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	41	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	104^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
chbBCARFG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1817809..1817849 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1736 (EcoliWiki Page)	NCBI GEO profiles for chbA
microarray	GenExpDB:b1736 (EcoliWiki Page)	Summary of data for chbA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1817758..1817949) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ17; Well:A6^[7]
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Notes

Accessions Related to chbA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10142	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0140	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1736
EcoGene	EcoGene:EG10142	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000138	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005792	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000033474 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	CELC	From SHIGELLACYC
E. coli O157	CELC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:46973
Pfam (EcoliWiki Page)	PF02255 PTS system, Lactose/Cellobiose specific IIA subunit
EcoCyc	EcoCyc:EG10142	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10142	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000138	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0140	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005792	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

chbA:On One Page

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Gene

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Notes

Location(s) and DNA Sequence

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Sequence Features

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Alleles and Phenotypes

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Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

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Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to chbA Expression

Notes

Links

Evolution

Homologs in Other Organisms

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Notes

Families

Notes

Links

References

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