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Standard Name	btuR
Gene Synonym(s)	ECK1264, b1270, JW1262, cobA^[1], cobA
Product Desc.	Regulatory gene affecting btuB^[2]
Product Synonyms(s)	cob(I)alamin adenolsyltransferase/cobinamide ATP-dependent adenolsyltransferase^[1], B1270^[3]^[1], CobA^[3]^[1], BtuR^[3]^[1] , cobA, ECK1264, JW1262, b1270
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): btuR^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	btuR
Mnemonic	B12 uptake
Synonyms	ECK1264, b1270, JW1262, cobA^[1], cobA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	28.58 minutes	MG1655: 1326381..1325791 <gbrowseImage> name=NC_000913:1325791..1326381 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1326729..1326139 <gbrowseImage> name=NC_012967:1326139..1326729 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1216640..1217230 <gbrowseImage> name=NC_012759:1216640..1217230 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1330071..1329481 <gbrowseImage> name=NC_007779:1329481..1330071 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1415777..1415187 <gbrowseImage> name=NC_010473:1415187..1415777 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Reference	Availability	Comments
ΔbtuR (Keio:JW1262)	deletion	deletion		PMID:16738554	Shigen CGSC9137^[4]
ΔbtuR778::kan				PMID:16738554	CGSC:100222
btuR510::Tn10dTc	Tn10dTc insertion 268 bp downstream of cobT translation start.		Auxotrophies	PMID:7592411		See Table 2 and Figure 1.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1262	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTGATGAACGCTACCAACA Primer 2:CCATAATCGATCCCTATCTGCGC
4F1	Kohara Phage	Genobase	PMID:3038334
13F9	Kohara Phage	Genobase	PMID:3038334
trpC83::Tn10	Linked marker	CAG18455 = CGSC7371^[4]	est. P1 cotransduction: 76% ^[5] Synonyms:trpB83::Tn10
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[4]	est. P1 cotransduction: 16% ^[5] Synonyms:zci-233::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10130	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10130	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000126	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945839	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0128	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004261	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BtuR
Synonyms	cob(I)alamin adenolsyltransferase/cobinamide ATP-dependent adenolsyltransferase^[1], B1270^[3]^[1], CobA^[3]^[1], BtuR^[3]^[1] , cobA, ECK1264, JW1262, b1270
Product description	Regulatory gene affecting btuB^[2]
EC number (for enzymes)	2.5.1.-^[1] 2.5.1.17^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003724	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008817	cob(I)yrinic acid a,c-diamide adenosyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003724	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008817	cob(I)yrinic acid a,c-diamide adenosyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.5.1.17	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009236	cobalamin biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003724	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009236	cobalamin biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0169	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009236	cobalamin biosynthetic process	PMID:7592411	IMP: Inferred from Mutant Phenotype		P	See Table 2	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	folB	PMID:16606699	Experiment(s):EBI-1139399
Protein	fdhD	PMID:16606699	Experiment(s):EBI-1139399
Protein	trpH	PMID:16606699	Experiment(s):EBI-1139399
Protein	rpsE	PMID:16606699	Experiment(s):EBI-1139399
Protein	yhjB	PMID:16606699	Experiment(s):EBI-1139399
Protein	yfgI	PMID:16606699	Experiment(s):EBI-1139399
Protein	eaeH	PMID:16606699	Experiment(s):EBI-1139399
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1139399
Protein	yecS	PMID:16606699	Experiment(s):EBI-1139399
Protein	cca	PMID:16606699	Experiment(s):EBI-1139399
Protein	idnO	PMID:16606699	Experiment(s):EBI-1139399
Protein	ribD	PMID:16606699	Experiment(s):EBI-1139399
Protein	ftsZ	PMID:16606699	Experiment(s):EBI-1139399
Protein	dicC	PMID:16606699	Experiment(s):EBI-1139399
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[6]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSDERYQQRQ QRVKEKVDAR VAQAQDERGI IIVFTGNGKG KTTAAFGTAT RAVGHGKKVG VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWDTQNRES DTAACREVWQ HAKRMLADSS LDMVLLDELT YMVAYDYLPL EEVVQALNER PHQQTVIITG RGCHRDILEL ADTVSELRPV KHAFDAGVKA QIGIDY
Length	196
Mol. Wt	21.997 kDa
pI	6.5 (calculated)
Extinction coefficient	23,950 - 24,200 (calc based on 5 Y, 3 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	26..196	PF02572 ATP:corrinoid adenosyltransferase BtuR/CobO/CobP	PMID:19920124
Domain	1..21	PF12557 Cob(I)alamin adenosyltransferase N terminal	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=btuR taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129231	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945839	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004261	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9H5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10130	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10130	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945839	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000126	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0128	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	257	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	130	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	184	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
btuR
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1326361..1326401 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1270 (EcoliWiki Page)	NCBI GEO profiles for btuR
microarray	GenExpDB:b1270 (EcoliWiki Page)	Summary of data for btuR from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to btuR Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10130	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0128	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1270
EcoGene	EcoGene:EG10130	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000126	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004261	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	BTUR	From SHIGELLACYC
E. coli O157	BTUR	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02572 ATP:corrinoid adenosyltransferase BtuR/CobO/CobP
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Pfam (EcoliWiki Page)	PF12557 Cob(I)alamin adenosyltransferase N terminal
EcoCyc	EcoCyc:EG10130	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10130	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000126	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0128	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004261	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

btuR:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to btuR Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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