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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	blc
Gene Synonym(s)	ECK4145, b4149, JW4110, yjeL^[1], yjeL
Product Desc.	Blc outer membrane lipoprotein (lipocalin)^[2]^[3] Outer membrane lipoprotein, cell division and growth function; prokaryotic lipocalin^[4]
Product Synonyms(s)	outer membrane lipoprotein (lipocalin)^[1], B4149^[2]^[1], YjeL^[2]^[1], Blc^[2]^[1] , ECK4145, JW4110, yjeL, b4149
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): blc^[2]
Regulation/Activity
Quick Links
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Notes

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RpoS regulon. AFMB Structural Genomics target No. 112 (http://afmb.cnrs-mrs.fr/article171.html). First 18 aa are a type II signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	blc
Mnemonic	Bacterial lipocalin
Synonyms	ECK4145, b4149, JW4110, yjeL^[1], yjeL
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	94.3 minutes	MG1655: 4375745..4375212 <gbrowseImage> name=NC_000913:4375212..4375745 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4356433..4355897 <gbrowseImage> name=NC_012967:4355897..4356433 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4313947..4314480 <gbrowseImage> name=NC_012759:4313947..4314480 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4382402..4381869 <gbrowseImage> name=NC_007779:4381869..4382402 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4476107..4475574 <gbrowseImage> name=NC_010473:4475574..4476107 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δblc (Keio:JW4110)	deletion	deletion	PMID:16738554	Shigen CGSC10959^[5]
Δblc-776::kan			PMID:16738554	CGSC:100206
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4110	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGCCTGCTCCCTCTCGTTGC Primer 2:CCACTACCAGGCTGCTGTACCCA
1D4	Kohara Phage	Genobase	PMID:3038334
3H6	Kohara Phage	Genobase	PMID:3038334
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 51% ^[6] Synonyms:zjd-2231::Tn10
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 9% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7837	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12474	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004333	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948670	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2367	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013590	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Blc
Synonyms	outer membrane lipoprotein (lipocalin)^[1], B4149^[2]^[1], YjeL^[2]^[1], Blc^[2]^[1] , ECK4145, JW4110, yjeL, b4149
Product description	Blc outer membrane lipoprotein (lipocalin)^[2]^[3] Outer membrane lipoprotein, cell division and growth function; prokaryotic lipocalin^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002345	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002446	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002345	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002446	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008289	lipid binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0446	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpmG	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplO	PMID:19402753	LCMS(ID Probability):99.6
Protein	gapA	PMID:19402753	LCMS(ID Probability):99.6
Protein	gadB	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplP	PMID:19402753	LCMS(ID Probability):99.6
Protein	eno	PMID:19402753	LCMS(ID Probability):99.6
Protein	groS	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplF	PMID:19402753	LCMS(ID Probability):99.6
Protein	ugpB	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsC	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpmB	PMID:19402753	LCMS(ID Probability):99.6
Protein	adhE	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsA	PMID:19402753	LCMS(ID Probability):99.6
Protein	hupA	PMID:19402753	LCMS(ID Probability):99.6
Protein	ahpC	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplB	PMID:19402753	LCMS(ID Probability):99.6
Protein	yfiD	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsJ	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsN	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplA	PMID:19402753	LCMS(ID Probability):99.6
Protein	pflB	PMID:19402753	LCMS(ID Probability):99.6
Protein	dps	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplY	PMID:19402753	LCMS(ID Probability):99.6
Protein	secB	PMID:19402753	LCMS(ID Probability):99.6
Protein	pgk	PMID:19402753	LCMS(ID Probability):99.6
Protein	purA	PMID:19402753	LCMS(ID Probability):99.6
Protein	fbaA	PMID:19402753	LCMS(ID Probability):99.6
Protein	luxS	PMID:19402753	LCMS(ID Probability):99.6
Protein	tig	PMID:19402753	LCMS(ID Probability):99.6
Protein	cspA	PMID:19402753	LCMS(ID Probability):99.3
Protein	pykF	PMID:19402753	LCMS(ID Probability):99.6
Protein	malE	PMID:19402753	LCMS(ID Probability):99.6
Protein	yfcZ	PMID:19402753	LCMS(ID Probability):99.5
Protein	ptsH	PMID:19402753	LCMS(ID Probability):99.5
Protein	aspA	PMID:19402753	LCMS(ID Probability):99.6
Protein	phoB	PMID:19402753	LCMS(ID Probability):99.0
Protein	fabD	PMID:19402753	LCMS(ID Probability):99.6
Protein	aceA	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Outer membrane	PMID:15174130	EchoLocation:blc
outer membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRLLPLVAAA TAAFLVVACS SPTPPRGVTV VNNFDAKRYL GTWYEIARFD HRFERGLEKV TATYSLRDDG GLNVINKGYN PDRGMWQQSE GKAYFTGAPT RAALKVSFFG PFYGGYNVIA LDREYRHALV CGPDRDYLWI LSRTPTISDE VKQEMLAVAT REGFDVSKFI WVQQPGS
Length	177
Mol. Wt	19.851 kDa
pI	9.0 (calculated)
Extinction coefficient	35,410 - 35,660 (calc based on 9 Y, 4 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-18	UniProt Manual:Signal Peptides	UniProt:P0A901
Domain	34..174	PF08212 Lipocalin-like domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=blc taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131974	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948670	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013590	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A901	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7837	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12474	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948670	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004333	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2367	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	28	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	378	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	3^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
blc
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4375725..4375765 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4149 (EcoliWiki Page)	NCBI GEO profiles for blc
microarray	GenExpDB:b4149 (EcoliWiki Page)	Summary of data for blc from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4375208..4375456) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ09; Well:B5^[7]
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Notes

Accessions Related to blc Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7837	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2367	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4149
EcoGene	EcoGene:EG12474	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004333	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013590	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000029258 (score: 1.000; bootstrap: 100%) ENSANGP00000010586 (score: 0.139) ENSANGP00000028083 (score: 0.113)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G58070 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000019702 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-051023-8 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0190794 (score: 1.000; bootstrap: 100%) DDB0190641 (score: 0.531)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0053126 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000011312 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000345179 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000024719 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:88056 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6Z9F9 (score: 1.000; bootstrap: 100%) Q8S9H0 (score: 0.590) Q6K623 (score: 0.587)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000144341 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000175177 (score: 0.547) NEWSINFRUP00000135728 (score: 0.286) NEWSINFRUP00000155674 (score: 0.209) NEWSINFRUP00000144340 (score: 0.132)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00016843001 (score: 1.000; bootstrap: 100%) GSTENP00025486001 (score: 0.212) GSTENP00010171001 (score: 0.134) GSTENP00037576001 (score: 0.113)	From Inparanoid:20070104
Shigella flexneri	BLC	From SHIGELLACYC
E. coli O157	BLC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:50814
Panther (EcoliWiki Page)	PTHR10612:SF7
Pfam (EcoliWiki Page)	PF08212 Lipocalin-like domain
EcoCyc	EcoCyc:G7837	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12474	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004333	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2367	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013590	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

blc:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to blc Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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