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Standard Name	atoD
Gene Synonym(s)	ECK2214, b2221, JW2215^[1], JW2215
Product Desc.	AtoD^[2]; acetyl-CoA:acetoacetyl-CoA transferase, α subunit^[3]; Component of α complex^[2]^[3]; acetoacetyl-CoA transferase^[2]^[3] Acetyl-CoA:acetoacetyl-CoA transferase beta-subunit^[4]
Product Synonyms(s)	acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit^[1], B2221^[2]^[1], AtoD^[2]^[1] , ECK2214, JW2215, b2221
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): atoDAEB^[2], OP00154, atoDAB
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	atoD
Mnemonic	Acetoacetate
Synonyms	ECK2214, b2221, JW2215^[1], JW2215
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	50.04 minutes	MG1655: 2321469..2322131 <gbrowseImage> name=NC_000913:2321469..2322131 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2270147..2270809 <gbrowseImage> name=NC_012967:2270147..2270809 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2207274..2207936 <gbrowseImage> name=NC_012759:2207274..2207936 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2328117..2328779 <gbrowseImage> name=NC_007779:2328117..2328779 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2412457..2413119 <gbrowseImage> name=NC_010473:2412457..2413119 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔatoD (Keio:JW2215)	deletion	deletion	PMID:16738554	Shigen CGSC9786^[5]
atoD32				CGSC:7504
atoD532				CGSC:16171
ΔatoD775::kan			PMID:16738554	CGSC:100128
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2215	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAACAAAATTGATGACATT Primer 2:CCTTTGCTCTCCTGTGAAACGAT
5A1	Kohara Phage	Genobase	PMID:3038334
4F12	Kohara Phage	Genobase	PMID:3038334
atoS298::Tn10	Linked marker	CAG12177 gyrA+ = CGSC7525^[5]	est. P1 cotransduction: 95% ^[6] Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).
zfa-723::Tn10	Linked marker	CAG12178 gyrA+ = CGSC7526^[5]	est. P1 cotransduction: 45% ^[6] Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11669	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001612	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947525	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1620	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007347	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AtoD
Synonyms	acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit^[1], B2221^[2]^[1], AtoD^[2]^[1] , ECK2214, JW2215, b2221
Product description	AtoD^[2]; acetyl-CoA:acetoacetyl-CoA transferase, α subunit^[3]; Component of α complex^[2]^[3]; acetoacetyl-CoA transferase^[2]^[3] Acetyl-CoA:acetoacetyl-CoA transferase beta-subunit^[4]
EC number (for enzymes)	2.8.3.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006629	lipid metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0443	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006631	fatty acid metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0276	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004163	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004165	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008410	CoA-transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004163	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008410	CoA-transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004165	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008410	CoA-transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012792	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008775	acetate CoA-transferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.8.3.8	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of α complex	could be indirect
Protein	groL		PMID:16606699	Experiment(s):EBI-1142218
Protein	Subunits of acetoacetyl-CoA transferase	could be indirect
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKTKLMTLQD ATGFFRDGMT IMVGGFMGIG TPSRLVEALL ESGVRDLTLI ANDTAFVDTG IGPLIVNGRV RKVIASHIGT NPETGRRMIS GEMDVVLVPQ GTLIEQIRCG GAGLGGFLTP TGVGTVVEEG KQTLTLDGKT WLLERPLRAD LALIRAHRCD TLGNLTYQLS ARNFNPLIAL AADITLVEPD ELVETGELQP DHIVTPGAVI DHIIVSQESK
Length	220
Mol. Wt	23.526 kDa
pI	5.0 (calculated)
Extinction coefficient	6,990 - 7,240 (calc based on 1 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..216	PF01144 Coenzyme A transferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=atoD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130158	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947525	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007347	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76458	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11669	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11669	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947525	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001612	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1620	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
atoDAEB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2321449..2321489 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2221 (EcoliWiki Page)	NCBI GEO profiles for atoD
microarray	GenExpDB:b2221 (EcoliWiki Page)	Summary of data for atoD from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to atoD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11669	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1620	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2221
EcoGene	EcoGene:EG11669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001612	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007347	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Pan troglodytes	ENSPTRP00000028846 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000020798 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01144 Coenzyme A transferase
Panther (EcoliWiki Page)	PTHR13707:SF3
Superfamily (EcoliWiki Page)	SUPERFAMILY:100950
EcoCyc	EcoCyc:EG11669	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001612	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1620	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007347	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

atoD:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to atoD Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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