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Standard Name	atoA
Gene Synonym(s)	ECK2215, b2222, JW2216^[1], JW2216
Product Desc.	AtoA^[2]; acetyl-CoA:acetoacetyl-CoA transferase, β subunit^[3]; Component of β complex^[2]^[3]; acetoacetyl-CoA transferase^[2]^[3]^[4] Acetate CoA-transferase^[5]
Product Synonyms(s)	acetyl-CoA:acetoacetyl-CoA transferase, beta subunit^[1], B2222^[2]^[1], AtoA^[2]^[1] , ECK2215, JW2216, b2222
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): atoDAEB^[2], OP00154, atoDAB
Regulation/Activity
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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	atoA
Mnemonic	Acetoacetate
Synonyms	ECK2215, b2222, JW2216^[1], JW2216
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	50.05 minutes	MG1655: 2322131..2322781 <gbrowseImage> name=NC_000913:2322131..2322781 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2270809..2271459 <gbrowseImage> name=NC_012967:2270809..2271459 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2207936..2208586 <gbrowseImage> name=NC_012759:2207936..2208586 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2328779..2329429 <gbrowseImage> name=NC_007779:2328779..2329429 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2413119..2413769 <gbrowseImage> name=NC_010473:2413119..2413769 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔatoA (Keio:JW2216)	deletion	deletion	PMID:16738554	Shigen CGSC9787^[6]
atoA514				CGSC:17101
ΔatoA776::kan			PMID:16738554	CGSC:100122
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2216	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGATGCGAAACAACGTATTGC Primer 2:CCTAAATCACCCCGTTGCGTATT
5A1	Kohara Phage	Genobase	PMID:3038334
4F12	Kohara Phage	Genobase	PMID:3038334
atoS298::Tn10	Linked marker	CAG12177 gyrA+ = CGSC7525^[6]	est. P1 cotransduction: 93% ^[7] Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).
zfa-723::Tn10	Linked marker	CAG12178 gyrA+ = CGSC7526^[6]	est. P1 cotransduction: 46% ^[7] Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11670	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11670	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001613	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946719	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1621	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007349	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AtoA
Synonyms	acetyl-CoA:acetoacetyl-CoA transferase, beta subunit^[1], B2222^[2]^[1], AtoA^[2]^[1] , ECK2215, JW2216, b2222
Product description	AtoA^[2]; acetyl-CoA:acetoacetyl-CoA transferase, β subunit^[3]; Component of β complex^[2]^[3]; acetoacetyl-CoA transferase^[2]^[3]^[4] Acetate CoA-transferase^[5]
EC number (for enzymes)	2.8.3.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006629	lipid metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0443	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008775	acetate CoA-transferase activity	PMID:4563344	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0006631	fatty acid metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0276	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004164	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004165	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008775	acetate CoA-transferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.8.3.8	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of β complex	could be indirect
Protein	yfiO		PMID:16606699	Experiment(s):EBI-1142221
Protein	Subunits of acetoacetyl-CoA transferase	could be indirect PMID: 3025185
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDAKQRIARR VAQELRDGDI VNLGIGLPTM VANYLPEGIH ITLQSENGFL GLGPVTTAHP DLVNAGGQPC GVLPGAAMFD SAMSFALIRG GHIDACVLGG LQVDEEANLA NWVVPGKMVP GMGGAMDLVT GSRKVIIAME HCAKDGSAKI LRRCTMPLTA QHAVHMLVTE LAVFRFIDGK MWLTEIADGC DLATVRAKTE ARFEVAADLN TQRGDL
Length	216
Mol. Wt	22.959 kDa
pI	5.9 (calculated)
Extinction coefficient	12,490 - 13,115 (calc based on 1 Y, 2 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..198	PF01144 Coenzyme A transferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=atoA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130159	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946719	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007349	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76459	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11670	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11670	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946719	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001613	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1621	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
atoDAEB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2322111..2322151 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2222 (EcoliWiki Page)	NCBI GEO profiles for atoA
microarray	GenExpDB:b2222 (EcoliWiki Page)	Summary of data for atoA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to atoA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11670	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1621	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2222
EcoGene	EcoGene:EG11670	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001613	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007349	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000020432 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000014285 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000025196 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00024272 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00007330 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000027433 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000011738 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040723-3 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040808-61 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0187790 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0035298 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10977-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000023917 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000196371 (score: 1.000; bootstrap: 100%) ENSP00000355412 (score: 0.697)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000014436 (score: 1.000; bootstrap: 100%) ENSMMUP00000003061 (score: 0.091)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000025379 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1914291 (score: 1.000; bootstrap: 100%) MGI:1891061 (score: 0.618) MGI:2664115 (score: 0.618)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000154626 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00009069001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000012254 (score: 1.000; bootstrap: 100%) ENSXETP00000012975 (score: 0.306) ENSXETP00000024619 (score: 0.156)	From Inparanoid:20070104
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01144 Coenzyme A transferase
Panther (EcoliWiki Page)	PTHR13707:SF22
Superfamily (EcoliWiki Page)	SUPERFAMILY:100950
EcoCyc	EcoCyc:EG11670	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11670	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001613	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1621	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007349	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Jenkins, LS & Nunn, WD (1987) Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system. J. Bacteriol. 169 42-52 PubMed
↑ ^5.0 ^5.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^6.0 ^6.1 ^6.2 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

atoA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to atoA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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