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Gene |

Product(s) |

Expression|

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Standard Name	argK
Gene Synonym(s)	ECK2914, b2918, JW2885, ygfD^[1], meaB, scpK?, ygfD
Product Desc.	arginine transport ATPase^[2]^[3] Required to convert succinate to propionate, Arg transport?; reported to have ATPase and protein kinase activity^[4]
Product Synonyms(s)	membrane ATPase/protein kinase^[1], B2918^[2]^[1], YgfD^[2]^[1], ArgK^[2]^[1] , ECK2914, JW2885, meaB, scpK?, ygfD, b2918
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): scpA-argK-ygfGH^[2]
Regulation/Activity
Quick Links
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Notes

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The role of ArgK in arginine transport needs to be reexamined. Characterization of the ArgK ortholog MeaB strongly suggests that ArgK is actually involved in protecting Mcm from inactivation and that ArgK may bind to Mcm. MeaB and Mcm are fused in some organisms. MeaB of M. extorquens binds and hydrolyzes GTP. ArgK is a homolog of the human disease gene MMAA, implicated in methylmalonic aciduria. Part of a four gene operon encoding enzymes converting succinate to propionate. ATPase, but not protein kinase, activity is reportedly required for arginine transport. ArgK does not have predicted transmembrane segments typical of a membrane protein.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	argK
Mnemonic	Arginine
Synonyms	ECK2914, b2918, JW2885, ygfD^[1], meaB, scpK?, ygfD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	65.97 minutes	MG1655: 3061009..3062004 <gbrowseImage> name=NC_000913:3061009..3062004 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2948738..2949733 <gbrowseImage> name=NC_012967:2948738..2949733 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2948157..2949152 <gbrowseImage> name=NC_012759:2948157..2949152 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3061643..3062638 <gbrowseImage> name=NC_007779:3061643..3062638 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3154879..3155874 <gbrowseImage> name=NC_010473:3154879..3155874 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
argKS68R		S68R	Auxotrophies	(in strain: RC101)			Strain variation; seeded from UniProt:P27254
argKT154A		T154A	Auxotrophies	(in strain: RC101)			Strain variation; seeded from UniProt:P27254
ΔargK (Keio:JW2885)	deletion	deletion	Auxotrophies		PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2885	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTAATGAAGCCACGCTGGC Primer 2:CCATCAAAATATTGCGTCTGGAT
1A2	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: % ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: 40% ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11445	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11445	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001409	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947412	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1415	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ArgK
Synonyms	membrane ATPase/protein kinase^[1], B2918^[2]^[1], YgfD^[2]^[1], ArgK^[2]^[1] , ECK2914, JW2885, meaB, scpK?, ygfD, b2918
Product description	arginine transport ATPase^[2]^[3] Required to convert succinate to propionate, Arg transport?; reported to have ATPase and protein kinase activity^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplB	PMID:16606699	Experiment(s):EBI-1144302
Protein	yphE	PMID:16606699	Experiment(s):EBI-1144302
Protein	serA	PMID:16606699	Experiment(s):EBI-1144302
Protein	groL	PMID:16606699	Experiment(s):EBI-1144302
Protein	sbm	PMID:18950999	Direct/co-IP
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MINEATLAES IRRLRQGERA TLAQAMTLVE SRHPRHQALS TQLLDAIMPY CGNTLRLGVT GTPGAGKSTF LEAFGMLLIR EGLKVAVIAV DPSSPVTGGS ILGDKTRMND LARAEAAFIR PVPSSGHLGG ASQRARELML LCEAAGYDVV IVETVGVGQS ETEVARMVDC FISLQIAGGG DDLQGIKKGL MEVADLIVIN KDDGDNHTNV AIARHMYESA LHILRRKYDE WQPRVLTCSA LEKRGIDEIW HAIIDFKTAL TASGRLQQVR QQQSVEWLRK QTEEEVLNHL FANEDFDRYY RQTLLAVKNN TLSPRTGLRQ LSEFIQTQYF D
Length	331
Mol. Wt	36.704 kDa
pI	6.5 (calculated)
Extinction coefficient	26,930 - 27,430 (calc based on 7 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	22..295	PF03308 ArgK protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=argK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130819	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947412	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009579	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P27254	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11445	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11445	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947412	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001409	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1415	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
scpA-argK-ygfGH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3060989..3061029 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2918 (EcoliWiki Page)	NCBI GEO profiles for argK
microarray	GenExpDB:b2918 (EcoliWiki Page)	Summary of data for argK from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to argK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11445	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1415	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2918
EcoGene	EcoGene:EG11445	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001409	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Bos taurus	ENSBTAP00000017005 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00033893 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00020169 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000011400 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000004727 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0202803 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000016195 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000281317 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000007115 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000000606 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1923805 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000028299 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000015508 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000133759 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00032866001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000013366 (score: 1.000; bootstrap: 83%)	From Inparanoid:20070104
Shigella flexneri	YGFD	From SHIGELLACYC
E. coli O157	YGFD	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03308 ArgK protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Panther (EcoliWiki Page)	PTHR23408:SF3
EcoCyc	EcoCyc:EG11445	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11445	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001409	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1415	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

argK:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to argK Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

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