arcB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

arcB

Gene Synonym(s)

ECK3200, b3210, JW5536[1], JW5536

Product Desc.

hybrid sensory histidine kinase in two-component regulatory system with response regulator ArcA [1]

Tripartite sensor/histidine protein kinase; repression of aerobic genes and activationof some anerobic genes under anaerobic growth conditions; phosphorylates response regulator protein (ArcA); has respones regulator and second transmitter domains[2]

Product Synonyms(s)

B3210[3][1], sensor kinase-phosphotransferase ArcB-Phis292[3][1], ECK3200, JW5536, b3210

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): arcB[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

arcB

Mnemonic

Aerobic respiration control

Synonyms

ECK3200, b3210, JW5536[1], JW5536

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.18 minutes 

MG1655: 3351047..3348711
<gbrowseImage> name=NC_000913:3348711..3351047 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3288260..3285924
<gbrowseImage> name=NC_012967:3285924..3288260 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3235859..3238195
<gbrowseImage> name=NC_012759:3235859..3238195 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3352880..3350544
<gbrowseImage> name=NC_007779:3350544..3352880 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3448792..3446456
<gbrowseImage> name=NC_010473:3446456..3448792 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

arcBH292Q

H292Q

Loss of activity

seeded from UniProt:P0AEC3

arcBD576A

D576A

Loss of activity

seeded from UniProt:P0AEC3

arcBH717Q

H717Q

Loss of activity

seeded from UniProt:P0AEC3

arcB(del) (Keio:JW5536)

deletion

deletion

PMID:16738554

Shigen
CGSC11593[4]

arcB41(del-ins,::FRT)

PMID:10829079

CGSC:65938

arcB40(del-ins)::cat

PMID:10829079

CGSC:65940

arcB738(del)::kan

PMID:16738554

CGSC:112242

arcB1

ECL619

Growth Phenotype

Codependant with arcB2 for pilus formation

PMID:2565334

arcB2

ECL620

Growth Phenotype

codependant with arcB1 for pilus formation

PMID:2565334

CAG45114 arcB738(del)::FRTKanFRT

Deletion

SigmaE activity

Increase in sigmaE activity

PMID:23224554

Parental Strain: CAG45114 Experimental Strain: SMR15364

See table S11 for full experimental results.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5536

Plasmid clone

Shigen

PMID:16769691

Status:cloned as old JW

Primer 1:GCCAAGCAAATTCGTCTGCTGGC

Primer 2:CCTTTTTTAGTGGCTTTTGCCAC

7E3

Kohara Phage

Genobase

PMID:3038334

3G10

Kohara Phage

Genobase

PMID:3038334

8D6

Kohara Phage

Genobase

PMID:3038334

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[4]

est. P1 cotransduction: 54% [5]
Synonyms:zgj-203::Tn10, zha-203::Tn10

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[4]

est. P1 cotransduction: 59% [5]
Synonyms:zha-6::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10062

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10062

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000058

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947887

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0060

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010537

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArcB

Synonyms

B3210[3][1], sensor kinase-phosphotransferase ArcB-Phis292[3][1], ECK3200, JW5536, b3210

Product description

hybrid sensory histidine kinase in two-component regulatory system with response regulator ArcA [1]

Tripartite sensor/histidine protein kinase; repression of aerobic genes and activationof some anerobic genes under anaerobic growth conditions; phosphorylates response regulator protein (ArcA); has respones regulator and second transmitter domains[2]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000155

two-component sensor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000700

F

Seeded from EcoCyc (v14.0)

complete

GO:0000155

two-component sensor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003661

F

Seeded from EcoCyc (v14.0)

complete

GO:0000155

two-component sensor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005467

F

Seeded from EcoCyc (v14.0)

complete

GO:0000155

two-component sensor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014409

F

Seeded from EcoCyc (v14.0)

complete

GO:0000155

two-component sensor activity

PMID:1512197

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0000156

two-component response regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001789

F

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000700

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001789

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008207

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014409

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0902

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

PMID:1512197

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003594

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001789

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013767

P

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014409

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

PMID:2201868

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0018106

peptidyl-histidine phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005467

P

Seeded from EcoCyc (v14.0)

complete

GO:0018106

peptidyl-histidine phosphorylation

PMID:1512197

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

ffh

PMID:19402753

LCMS(ID Probability):99.6

Protein

glyA

PMID:19402753

LCMS(ID Probability):99.6

Protein

argG

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV ESIDVIRSIF
FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER DLSLNVQLKD NIAQLNQEIA
VREKAEAELQ ETFGQLKIEI KEREETQIQL EQQSSFLRSF LDASPDLVFY RNEDKEFSGC
NRAMELLTGK SEKQLVHLKP ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA
CFEIRKVPYY DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG
IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ LDNQPVDFTS
FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR QILWNLISNA VKFTQQGQVT
VRVRYDEGDM LHFEVEDSGI GIPQDELDKI FAMYYQVKDS HGGKPATGTG IGLAVSRRLA
KNMGGDITVT SEQGKGSTFT LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV
ARSVLEKLGN SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL
PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE STVTTEENSK
SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVSVL ESNLTAQDKK GIVEEGHKIK
GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV GEWIEEMKEE WRHDVEVLKA WVAKATKK
Length

778

Mol. Wt

87.984 kDa

pI

4.9 (calculated)

Extinction coefficient

72,310 - 72,560 (calc based on 19 Y, 8 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

394..506

PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

PMID:19920124

Domain

282..347

PF00512 His Kinase A (phosphoacceptor) domain

PMID:19920124

Domain

684..771

PF01627 Hpt domain

PMID:19920124

Domain

155..266

PF00989 PAS fold

PMID:19920124

Domain

528..640

PF00072 Response regulator receiver domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=arcB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176326

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947887

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010537

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AEC3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10062

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10062

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947887

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000058

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0060

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

9.99E+01

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

55.757+/-0.301

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.042808219

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

226

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

97

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

131

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

arcB

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3351027..3351067 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3210 (EcoliWiki Page)

NCBI GEO profiles for arcB

microarray

GenExpDB:b3210 (EcoliWiki Page)

Summary of data for arcB from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3350582..3350820) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:G6[6]

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Notes

Accessions Related to arcB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10062

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0060

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3210

EcoGene

EcoGene:EG10062

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000058

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010537

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

ARCB

From SHIGELLACYC

E. coli O157

ARCB

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00512 His Kinase A (phosphoacceptor) domain

Pfam (EcoliWiki Page)

PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Pfam (EcoliWiki Page)

PF01627 Hpt domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:47226

Superfamily (EcoliWiki Page)

SUPERFAMILY:47384

Superfamily (EcoliWiki Page)

SUPERFAMILY:52172

Panther (EcoliWiki Page)

PTHR24423:SF67

Superfamily (EcoliWiki Page)

SUPERFAMILY:55785

Superfamily (EcoliWiki Page)

SUPERFAMILY:55874

Pfam (EcoliWiki Page)

PF00989 PAS fold

Pfam (EcoliWiki Page)

PF00072 Response regulator receiver domain

EcoCyc

EcoCyc:EG10062

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10062

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000058

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0060

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010537

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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