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Standard Name	apaG
Gene Synonym(s)	ECK0051, b0050, JW0049, temP^[1], corD, temP
Product Desc.	hypothetical protein^[2]^[3] Expressed as part of complex ksgA operon, function unknown; Salmonella ortholog involved in Co2+ and Mg2+ efflux; upstream of apaH^[4]
Product Synonyms(s)	protein associated with Co2+ and Mg2+ efflux^[1], B0050^[2]^[1], ApaG^[2]^[1] , corD, ECK0051, JW0049, temP, b0050
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): apaGH^[2], pdxA-rsmA-apaGH^[2], pdxA-ksgA-apaG-apaH, rsmA-apaGH^[2], ksgA-apaG-apaH, rsmA-apaG-apaH, surA-pdxA-rsmA-apaGH^[2], surA-pdxA-ksgA-apaGH
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	apaG
Mnemonic	Adenine-tetraPhosphate-adenine
Synonyms	ECK0051, b0050, JW0049, temP^[1], corD, temP
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
BW2952		NC_012759: 51229..51606 <gbrowseImage> name=NC_012759:51229..51606 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
MG1655	1.1 minutes	MG1655: 51606..51229 <gbrowseImage> name=NC_000913:51229..51606 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 55801..55424 <gbrowseImage> name=NC_012967:55424..55801 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 51606..51229 <gbrowseImage> name=NC_007779:51229..51606 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 51606..51229 <gbrowseImage> name=NC_010473:51229..51606 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
apaG(del) (Keio:JW0049)	deletion	deletion			PMID:16738554	Shigen
apaG762(del)::FRT			Mutagenesis rate	Decreased stress-induced mutagenesis (SIM) phenotype.	PMID:23224554	Parental Strain: SMR4562 Experimental Strain: SMR12241	Mutation was comparatively weak with decrease only happening to 33-67% of population.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0049	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATCAATTCGCCCCGAGTGTG Primer 2:CCATGAATGAGTGTGGGAACGGC
2F7	Kohara Phage	Genobase	PMID:3038334
4A3	Kohara Phage	Genobase	PMID:3038334
8D2	Kohara Phage	Genobase	PMID:3038334
carB96::Tn10	Linked marker	CAG12093 = CGSC7322^[5]	est. P1 cotransduction: 51% ^[6] Synonyms:car-96::Tn10
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 28% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10047	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10047	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000043	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944772	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0045	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000169	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ApaG
Synonyms	protein associated with Co2+ and Mg2+ efflux^[1], B0050^[2]^[1], ApaG^[2]^[1] , corD, ECK0051, JW0049, temP, b0050
Product description	hypothetical protein^[2]^[3] Expressed as part of complex ksgA operon, function unknown; Salmonella ortholog involved in Co2+ and Mg2+ efflux; upstream of apaH^[4]
EC number (for enzymes)
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Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MINSPRVCIQ VQSVYIEAQS SPDNERYVFA YTVTIRNLGR APVQLLGRYW LITNGNGRET EVQGEGVVGV QPLIAPGEEY QYTSGAIIET PLGTMQGHYE MIDENGVPFS IDIPVFRLAV PTLIH
Length	125
Mol. Wt	13.866 kDa
pI	4.5 (calculated)
Extinction coefficient	15,930 - 16,055 (calc based on 7 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	16..105	PF04379 Protein of unknown function (DUF525)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=apaG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128044	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000169	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P62672	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10047	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10047	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944772	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000043	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944772	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0045	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.82+/-0.071	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.086206897	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	113	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	260	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	89^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
apaGH
pdxA-rsmA-apaGH
rsmA-apaGH
surA-pdxA-rsmA-apaGH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:51586..51626 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0050 (EcoliWiki Page)	NCBI GEO profiles for apaG
microarray	GenExpDB:b0050 (EcoliWiki Page)	Summary of data for apaG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to apaG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10047	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0045	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0050
EcoGene	EcoGene:EG10047	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000043	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000169	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000012066 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000013287 (score: 1.000; bootstrap: 83%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000010637 (score: 1.000; bootstrap: 82%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000016033 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-030131-5881 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-670 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0218369 (score: 1.000; bootstrap: 100%) DDB0218063 (score: 0.052)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0037329 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA11446-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000012576 (score: 1.000; bootstrap: 80%) ENSGALP00000010670 (score: 1.000; bootstrap: 81%)	From Inparanoid:20070104
Homo sapiens	ENSP00000265651 (score: 1.000; bootstrap: 83%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000007964 (score: 1.000; bootstrap: 83%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000012305 (score: 1.000; bootstrap: 83%)	From Inparanoid:20070104
Mus musculus	MGI:1929084 (score: 1.000; bootstrap: 83%)	From Inparanoid:20070104
Oryza gramene	Q65XS0 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000006059 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000014478 (score: 1.000; bootstrap: 84%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000142251 (score: 1.000; bootstrap: 60%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00012551001 (score: 1.000; bootstrap: 64%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000008233 (score: 1.000; bootstrap: 69%)	From Inparanoid:20070104
Shigella flexneri	APAG	From SHIGELLACYC
E. coli O157	APAG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04379 Protein of unknown function (DUF525)
Panther (EcoliWiki Page)	PTHR14289:SF15
Superfamily (EcoliWiki Page)	SUPERFAMILY:110069
EcoCyc	EcoCyc:EG10047	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10047	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000043	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0045	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000169	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 ^2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

apaG:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to apaG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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