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Expression|

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Standard Name	allC
Gene Synonym(s)	ECK0509, b0516, JW0504, ylbB, glxB7^[1], glxB7
Product Desc.	G6285^[2]^[3] Allantoate amidohydrolase: allantoin for anaerobic nitrogen^[4]
Product Synonyms(s)	allantoate amidohydrolase^[1], B0516^[2]^[1], YlbB^[2]^[1], AllC^[2]^[1] , ECK0509, glxB7, JW0504, ylbB, b0516
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): allDC-ylbA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	allC
Mnemonic	Allantoin
Synonyms	ECK0509, b0516, JW0504, ylbB, glxB7^[1], glxB7
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	11.71 minutes	MG1655: 544516..543281 <gbrowseImage> name=NC_000913:543281..544516 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 517458..516223 <gbrowseImage> name=NC_012967:516223..517458 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 446040..447275 <gbrowseImage> name=NC_012759:446040..447275 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 544516..543281 <gbrowseImage> name=NC_007779:543281..544516 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 483847..482612 <gbrowseImage> name=NC_010473:482612..483847 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

allC(del) (Keio:JW0504)

deletion

PMID:16738554

Shigen

CGSC8648^[5]

allC728(del)::kan

PMID:16738554

CGSC:99974

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0504	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTACACATTTCCGTCAAGC Primer 2:CCTTTCTGCCAGGCAAGTTGATA
9E5	Kohara Phage	Genobase	PMID:3038334
6E7	Kohara Phage	Genobase	PMID:3038334
gsk-3055::Tn10	Linked marker	CAG12154 = CGSC7341^[5]	est. P1 cotransduction: 16% ^[6] Synonyms:zbb-30-55::TnlO, zba-3055::Tn10
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 74% ^[6] Synonyms:purE79::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6285	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13623	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002893	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945150	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3388	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001777	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AllC
Synonyms	allantoate amidohydrolase^[1], B0516^[2]^[1], YlbB^[2]^[1], AllC^[2]^[1] , ECK0509, glxB7, JW0504, ylbB, b0516
Product description	G6285^[2]^[3] Allantoate amidohydrolase: allantoin for anaerobic nitrogen^[4]
EC number (for enzymes)	3.5.3.4^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006144	purine base metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0659	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002933	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008237	metallopeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002933	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009442	allantoin assimilation pathway	PMID:19935661	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	PMID:20038185	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0042803	protein homodimerization activity	PMID:17362992	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0047652	allantoate deiminase activity	PMID:20038185	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	uvrY	PMID:16606699	Experiment(s):EBI-1136995
Protein	rpsT	PMID:16606699	Experiment(s):EBI-1136995
Protein	nadE	PMID:16606699	Experiment(s):EBI-1136995
Protein	kbaZ	PMID:16606699	Experiment(s):EBI-1136995
Protein	talB	PMID:16606699	Experiment(s):EBI-1136995
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MITHFRQAIE ETLPWLSSFG ADPAGGMTRL LYSPEWLETQ QQFKKRMAAS GLETRFDEVG NLYGRLNGTE YPQEVVLSGS HIDTVVNGGN LDGQFGALAA WLAIDWLKTQ YGAPLRTVEV VAMAEEEGSR FPYVFWGSKN IFGLANPDDV RNICDAKGNS FVDAMKACGF TLPNAPLTPR QDIKAFVELH IEQGCVLESN GQSIGVVNAI VGQRRYTVTL NGESNHAGTT PMGYRRDTVY AFSRICHQSV EKAKRMGDPL VLTFGKVEPR PNTVNVVPGK TTFTIDCRHT DAAVLRDFTQ QLENDMRAIC DEMDIGIDID LWMDEEPVPM NKELVATLTE LCEREKLNYR VMHSGAGHDA QIFAPRVPTC MIFIPSINGI SHNPAERTNI TDLAEGVKTL ALMLYQLAWQ K
Length	411
Mol. Wt	45.693 kDa
pI	5.2 (calculated)
Extinction coefficient	53,400 - 54,400 (calc based on 10 Y, 7 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	77..406	PF01546 Peptidase family M20/M25/M40		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=allC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128500	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945150	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001777	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77425	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6285	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13623	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945150	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002893	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3388	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	1.03+/-0.033	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.004453441	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	9^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	6	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
allDC-ylbA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:544496..544536 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0516 (EcoliWiki Page)	NCBI GEO profiles for allC
microarray	GenExpDB:b0516 (EcoliWiki Page)	Summary of data for allC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to allC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6285	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3388	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0516
EcoGene	EcoGene:EG13623	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002893	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001777	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G43600 (score: 1.000; bootstrap: 57%)	From Inparanoid:20070104
Oryza gramene	Q655X8 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	YLBB	From SHIGELLACYC
E. coli O157	YLBB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01546 Peptidase family M20/M25/M40
Panther (EcoliWiki Page)	PTHR12053:SF3
Superfamily (EcoliWiki Page)	SUPERFAMILY:53187
Superfamily (EcoliWiki Page)	SUPERFAMILY:55031
EcoCyc	EcoCyc:G6285	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13623	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002893	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3388	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001777	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

allC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to allC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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