adhP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

adhP

Gene Synonym(s)

ECK1472, b1478, JW1474, yddN[1], yddN

Product Desc.

ADHP[2][3]

Ethanol-active dehydrogenase/acetaldehyde-active reductase[4]

Product Synonyms(s)

alcohol dehydrogenase, 1-propanol preferring[1], B1478[2][1], YddN[2][1], AdhP[2][1] , ECK1472, JW1474, yddN, b1478

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): adhP[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Medium chain dehydrogenase/reductase (MDR) activity reported by Shafqat (1999). Propanol substrate characterization and N-terminal sequence cited in SwissProt P39451 (S. Harayama, unpublished, 1994).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

adhP

Mnemonic

Alcohol/acetaldehyde dehydrogenase

Synonyms

ECK1472, b1478, JW1474, yddN[1], yddN

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

33.43 minutes 

MG1655: 1551862..1550852
<gbrowseImage> name=NC_000913:1550852..1551862 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1527776..1526766
<gbrowseImage> name=NC_012967:1526766..1527776 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1442911..1443921
<gbrowseImage> name=NC_012759:1442911..1443921 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1555552..1554542
<gbrowseImage> name=NC_007779:1554542..1555552 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1642457..1641447
<gbrowseImage> name=NC_010473:1641447..1642457 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔadhP (Keio:JW1474)

deletion

deletion

PMID:16738554

Shigen
CGSC9282[5]

ΔadhP771::kan

PMID:16738554

CGSC:99916


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1474

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAGGCTGCAGTTGTTACGAA

Primer 2:CCGTGACGGAAATCAATCACCAT

1G9

Kohara Phage

Genobase

PMID:3038334

9B8

Kohara Phage

Genobase

PMID:3038334

10C7

Kohara Phage

Genobase

PMID:3038334

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[5]

est. P1 cotransduction: 59% [6]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: % [6]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6775

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12622

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003364

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946036

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2506

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004928

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AdhP

Synonyms

alcohol dehydrogenase, 1-propanol preferring[1], B1478[2][1], YddN[2][1], AdhP[2][1] , ECK1472, JW1474, yddN, b1478

Product description

ADHP[2][3]

Ethanol-active dehydrogenase/acetaldehyde-active reductase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0004022

alcohol dehydrogenase (NAD) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002085

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002328

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013149

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002085

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002328

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013149

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013154

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002085

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002328

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013149

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

NOT

GO:0047639

alcohol oxidase activity

PMID:10406936

IDA: Inferred from Direct Assay

F

The term definition for GO:0047639 specifies O2 as the reductant in the reaction, which is not the reaction catalyzed by AdhP.

complete

GO:0005625

soluble fraction

PMID:10406936

IDA: Inferred from Direct Assay

C

complete

GO:0045471

response to ethanol

PMID:10406936

IEP: Inferred from Expression Pattern

P

complete

GO:0046187

acetaldehyde catabolic process

PMID:10406936

IDA: Inferred from Direct Assay

P

complete

GO:0004022

alcohol dehydrogenase (NAD) activity

PMID:10406936

IDA: Inferred from Direct Assay

F

Table 2

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1139945

Protein

fusA

PMID:16606699

Experiment(s):EBI-1139945

Protein

aceB

PMID:16606699

Experiment(s):EBI-1139945
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MQNIIRKGGT MKAAVVTKDH HVDVTYKTLR SLKHGEALLK MECCGVCHTD LHVKNGDFGD
KTGVILGHEG IGVVAEVGPG VTSLKPGDRA SVAWFYEGCG HCEYCNSGNE TLCRSVKNAG
YSVDGGMAEE CIVVADYAVK VPDGLDSAAA SSITCAGVTT YKAVKLSKIR PGQWIAIYGL
GGLGNLALQY AKNVFNAKVI AIDVNDEQLK LATEMGADLA INSHTEDAAK IVQEKTGGAH
AAVVTAVAKA AFNSAVDAVR AGGRVVAVGL PPESMSLDIP RLVLDGIEVV GSLVGTRQDL
TEAFQFAAEG KVVPKVALRP LADINTIFTE MEEGKIRGRM VIDFRH
Length

346

Mol. Wt

36.477 kDa

pI

6.6 (calculated)

Extinction coefficient

22,920 - 24,045 (calc based on 8 Y, 2 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

172..299

PF00107 Zinc-binding dehydrogenase

PMID:19920124

Domain

24..131

PF08240 Alcohol dehydrogenase GroES-like domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=adhP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111280

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946036

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004928

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P39451

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6775

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12622

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946036

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003364

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2506

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Expression of AdhP increases in response to ethanol.[7]

Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

20

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

773

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

21a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

adhP

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1551842..1551882 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1478 (EcoliWiki Page)

NCBI GEO profiles for adhP

microarray

GenExpDB:b1478 (EcoliWiki Page)

Summary of data for adhP from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to adhP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6775

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2506

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1478

EcoGene

EcoGene:EG12622

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003364

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004928

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Caenorhabditis briggsae

  • WBGene00041628 (score: 1.000; bootstrap: 99%)
  • WBGene00041627 (score: 0.426)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00010790 (score: 1.000; bootstrap: 99%)
  • WBGene00010791 (score: 0.668)
  • WBGene00017060 (score: 0.514)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000017957 (score: 1.000; bootstrap: 82%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YMR303C (score: 1.000; bootstrap: 99%)
  • YOL086C (score: 0.913)
  • YBR145W (score: 0.728)
  • YMR083W (score: 0.724)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC13B111 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ADHP

From SHIGELLACYC

E. coli O157

ADHP

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00107 Zinc-binding dehydrogenase

Superfamily (EcoliWiki Page)

SUPERFAMILY:50129

Superfamily (EcoliWiki Page)

SUPERFAMILY:51735

Panther (EcoliWiki Page)

PTHR11695:SF295

Pfam (EcoliWiki Page)

PF08240 Alcohol dehydrogenase GroES-like domain

EcoCyc

EcoCyc:G6775

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12622

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003364

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2506

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004928

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Shafqat, J et al. (1999) An ethanol-inducible MDR ethanol dehydrogenase/acetaldehyde reductase in Escherichia coli: structural and enzymatic relationships to the eukaryotic protein forms. Eur. J. Biochem. 263 305-11 PubMed

Categories

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