accD:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

accD

Gene Synonym(s)

ECK2310, b2316, JW2313, dedB[1], dedB, usg

Product Desc.

AccD[2][3];

Component of carboxyl transferase subunit β[2][3]; acetyl-CoA carboxyltransferase[2][3]; acetyl CoA carboxylase[2][3]

Acetyl-CoA carboxylase, carboxyltransferase beta subunit[4]

Product Synonyms(s)

acetyl-CoA carboxylase, beta (carboxyltranferase) subunit[1], B2316[2][1], DedB[2][1], AccD[2][1] , dedB, ECK2310, JW2313, usg, b2316

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): accD[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

accD

Mnemonic

Acetyl-CoA carboxylase

Synonyms

ECK2310, b2316, JW2313, dedB[1], dedB, usg

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

BW2952

NC_012759: 2316839..2317753
<gbrowseImage> name=NC_012759:2316839..2317753 source=BW2952 preset=GeneLocation </gbrowseImage>

MG1655

52.4 minutes, 52.4 minutes 

MG1655: 2431948..2431034
<gbrowseImage> name=NC_000913:2431034..2431948 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2377518..2376604
<gbrowseImage> name=NC_012967:2376604..2377518 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2439372..2438458
<gbrowseImage> name=NC_007779:2438458..2439372 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2523713..2522799
<gbrowseImage> name=NC_010473:2522799..2523713 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

accD6(ts)

Growth Phenotype

temperature sensitive

PMID:4564200 PMID:4597230 PMID:1355086

CGSC:64607

at 37C normal growth can be restored

accD ++

Overexpression

Increase Acetyle COA Carboxylase activity upon over expression.

PMID:10893421

See Table 1.

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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2313

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCTGGATTGAACGAATTAA

Primer 2:CCGGCCTCAGGTTCCTGATCCGG

9D2

Kohara Phage

Genobase

PMID:3038334

12F12

Kohara Phage

Genobase

PMID:3038334

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[5]

est. P1 cotransduction: 17% [6]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[5]

est. P1 cotransduction: 4% [6]
Synonyms:zfb-1::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10217

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10217

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000211

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946796

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0213

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007648

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AccD

Synonyms

acetyl-CoA carboxylase, beta (carboxyltranferase) subunit[1], B2316[2][1], DedB[2][1], AccD[2][1] , dedB, ECK2310, JW2313, usg, b2316

Product description

AccD[2][3];

Component of carboxyl transferase subunit β[2][3]; acetyl-CoA carboxyltransferase[2][3]; acetyl CoA carboxylase[2][3]

Acetyl-CoA carboxylase, carboxyltransferase beta subunit[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:19965770

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0003729

mRNA binding

PMID:19965770

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0003989

acetyl-CoA carboxylase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01395

F

Seeded from EcoCyc (v14.0)

complete

GO:0003989

acetyl-CoA carboxylase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000438

F

Seeded from EcoCyc (v14.0)

complete

GO:0003989

acetyl-CoA carboxylase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.4.1.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P0ABD5

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01395

P

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000438

P

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0275

P

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

PMID:10893421

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:16460018

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:19965770

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0009329

acetate CoA-transferase complex

PMID:1355089

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000022

F

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011762

F

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc (v14.0)

complete

GO:0017148

negative regulation of translation

PMID:19965770

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0003989

acetyl-CoA carboxylase activity

PMID:10893421

IDA: Inferred from Direct Assay

F

complete

GO:0006633

fatty acid biosynthetic process

PMID:10893421

IGI: Inferred from Genetic Interaction

EcoliWiki:accA EcoliWiki:accB EcoliWiki:accC


P

complete

GO:0042759

long-chain fatty acid biosynthetic process

PMID:18156466

NAS: Non-traceable Author Statement

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of carboxyl transferase subunit β

could be indirect

Protein

dnaK

PMID:15690043

Experiment(s):EBI-890114

Protein

pepD

PMID:15690043

Experiment(s):EBI-890114

Protein

pgm

PMID:15690043

Experiment(s):EBI-890114

Protein

rfaI

PMID:15690043

Experiment(s):EBI-890114

Protein

rplU

PMID:15690043

Experiment(s):EBI-890114

Protein

rplV

PMID:15690043

Experiment(s):EBI-890114

Protein

rpmG

PMID:15690043

Experiment(s):EBI-890114

Protein

mdtB

PMID:15690043

Experiment(s):EBI-890114

Protein

yhdA

PMID:15690043

Experiment(s):EBI-890114

Protein

accA

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

Subunits of acetyl-CoA carboxyltransferase

could be indirect

Protein

Subunits of acetyl CoA carboxylase

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSWIERIKSN ITPTRKASIP EGVWTKCDSC GQVLYRAELE RNLEVCPKCD HHMRMTARNR
LHSLLDEGSL VELGSELEPK DVLKFRDSKK YKDRLASAQK ETGEKDALVV MKGTLYGMPV
VAAAFEFAFM GGSMGSVVGA RFVRAVEQAL EDNCPLICFS ASGGARMQEA LMSLMQMAKT
SAALAKMQER GLPYISVLTD PTMGGVSASF AMLGDLNIAE PKALIGFAGP RVIEQTVREK
LPPGFQRSEF LIEKGAIDMI VRRPEMRLKL ASILAKLMNL PAPNPEAPRE GVVVPPVPDQ EPEA
Length

304

Mol. Wt

33.322 kDa

pI

7.7 (calculated)

Extinction coefficient

16,960 - 17,710 (calc based on 4 Y, 2 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

51..302

PF01039 Carboxyl transferase domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=accD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130251

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946796

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007648

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A9Q5

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10217

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10217

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946796

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000211

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0213

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

8.05E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

8774

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2475

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4611

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

accD

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2431928..2431968 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2316 (EcoliWiki Page)

NCBI GEO profiles for accD

microarray

GenExpDB:b2316 (EcoliWiki Page)

Summary of data for accD from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to accD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10217

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0213

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2316

EcoGene

EcoGene:EG10217

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000211

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007648

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000000860 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000020252 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000006126 (score: 0.093)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00034728 (score: 1.000; bootstrap: 100%)
  • WBGene00029023 (score: 0.180)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00018701 (score: 1.000; bootstrap: 100%)
  • WBGene00008514 (score: 0.174)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000010760 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000011689 (score: 0.158)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000016122 (score: 1.000; bootstrap: 100%)
  • ENSCINP00000018880 (score: 0.163)
  • ENSCINP00000005637 (score: 0.083)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040426-2467 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-3414 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-3440 (score: 0.149)
  • ZDB-GENE-040426-2493 (score: 0.149)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0166993 (score: 1.000; bootstrap: 100%)
  • DDB0168580 (score: 0.172)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA17055-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000001845 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000003974 (score: 0.104)
  • ENSGALP00000018342 (score: 0.102)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000251654 (score: 1.000; bootstrap: 100%)
  • ENSP00000343657 (score: 0.149)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000023102 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000002007 (score: 0.143)
  • ENSMODP00000000863 (score: 0.092)

From Inparanoid:20070104

Mus musculus

  • MGI:1914154 (score: 1.000; bootstrap: 100%)
  • MGI:1925288 (score: 0.152)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000021657 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000023900 (score: 0.136)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000137640 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000176970 (score: 0.098)
  • NEWSINFRUP00000128319 (score: 0.085)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00012729001 (score: 1.000; bootstrap: 100%)
  • GSTENP00029632001 (score: 0.101)
  • GSTENP00023172001 (score: 0.080)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000014779 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ACCD

From SHIGELLACYC

E. coli O157

ACCD

From ECOO157CYC

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01039 Carboxyl transferase domain


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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