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Expression|

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Standard Name	aat
Gene Synonym(s)	ECK0876, b0885, JW0868, ycaA, G8002^[1]^[2]
Product Desc.	leucyl, phenylalanyl-tRNA-protein transferase^[2]^[3] Leucyl, phenylalanyl-tRNA-protein transferase; L/F-transferase; transfers Leu or Phe to N-terminal Arg or Lys residues; required for the N-end rule degradation by ClpAP protease^[4]
Product Synonyms(s)	leucyl/phenylalanyl-tRNA-protein transferase^[1], B0885^[2]^[1], YcaA^[2]^[1], Aat^[2]^[1] , ECK0876, JW0868, ycaA, b0885
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aat^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aat
Mnemonic	Amino acyl-tRNA-protein transferase
Synonyms	ECK0876, b0885, JW0868, ycaA, G8002^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	19.96 minutes	MG1655: 926655..925951 <gbrowseImage> name=NC_000913:925951..926655 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 944490..943786 <gbrowseImage> name=NC_012967:943786..944490 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 828919..829623 <gbrowseImage> name=NC_012759:828919..829623 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 927854..927150 <gbrowseImage> name=NC_007779:927150..927854 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 980583..979879 <gbrowseImage> name=NC_010473:979879..980583 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
Δaat (Keio:JW0868)	deletion	deletion			PMID:16738554	Shigen CGSC8899^[5]
aat-1				MS845 has only 50% the tryptophanase activity of parent strain.	PMID:137233	CGSC:8542
Δaat-784::kan					PMID:16738554	CGSC:99874
aat-1			Growth Phenotype	When aspartate was the sole nitrogen source, the 'aat-1' mutant strain (MS845 F'128) grew about twice as fast as its parent (doubling times of 2.3 and 4.8 h, respectively).	PMID:137233	CGSC:8542
att-1			Cell Shape	Abnormal Morphology appearing as clubs, spheres or ellipsoids.	PMID:137233	Parental Strain: W4977 Experimental Strain: MS845	See table 1. Normal rod-shaped morphology was restored by addition of D-alanine or D-glutamic acid to the medium
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0868	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGCCTGGTTCAGCTTTCTCG Primer 2:CCTTCTTGTGGTGAAAACAAGCA
1H1	Kohara Phage	Genobase	PMID:3038334
1F10	Kohara Phage	Genobase	PMID:3038334
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 47% ^[6] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11112	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11112	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001098	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945490	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1103	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003009	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Aat
Synonyms	leucyl/phenylalanyl-tRNA-protein transferase^[1], B0885^[2]^[1], YcaA^[2]^[1], Aat^[2]^[1] , ECK0876, JW0868, ycaA, b0885
Product description	leucyl, phenylalanyl-tRNA-protein transferase^[2]^[3] Leucyl, phenylalanyl-tRNA-protein transferase; L/F-transferase; transfers Leu or Phe to N-terminal Arg or Lys residues; required for the N-end rule degradation by ClpAP protease^[4]
EC number (for enzymes)	2.3.2.6^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00688	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0030163	protein catabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00688	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030163	protein catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004616	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008914	leucyltransferase activity	PMID:4909560	IDA: Inferred from Direct Assay		F		complete
	GO:0008914	leucyltransferase activity	PMID:7657641	IDA: Inferred from Direct Assay		F	Table 1	complete
	GO:0005737	cytoplasm	PMID:4909560	IDA: Inferred from Direct Assay		C		complete
	GO:0016755	transferase activity, transferring amino-acyl groups	PMID:4909560	IDA: Inferred from Direct Assay		F	Aat catalyzes the transfer of both leucine and phenylalanine from tRNAs to protein. Specificity for leucyl- and phenyl-tRNAs is presented in Table II.	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	yjdA	PMID:16606699	Experiment(s):EBI-1138274
Protein	infA	PMID:16606699	Experiment(s):EBI-1138274
Protein	bglX	PMID:16606699	Experiment(s):EBI-1138274
Protein	speA	PMID:16606699	Experiment(s):EBI-1138274
Protein	groL	PMID:16606699	Experiment(s):EBI-1138274
Protein	ninE	PMID:16606699	Experiment(s):EBI-1138274
Protein	kdpB	PMID:16606699	Experiment(s):EBI-1138274
Protein	galF	PMID:19402753	MALDI(Z-score):28.162890
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRLVQLSRHS IAFPSPEGAL REPNGLLALG GDLSPARLLM AYQRGIFPWF SPGDPILWWS PDPRAVLWPE SLHISRSMKR FHKRSPYRVT MNYAFGQVIE GCASDREEGT WITRGVVEAY HRLHELGHAH SIEVWREDEL VGGMYGVAQG TLFCGESMFS RMENASKTAL LVFCEEFIGH GGKLIDCQVL NDHTASLGAC EIPRRDYLNY LNQMRLGRLP NNFWVPRCLF SPQE
Length	234
Mol. Wt	26.618 kDa
pI	7.2 (calculated)
Extinction coefficient	48,930 - 49,680 (calc based on 7 Y, 7 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	34..204	PF03588 Leucyl/phenylalanyl-tRNA protein transferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aat taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128852	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945490	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003009	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A8P1	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11112	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11112	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945490	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001098	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1103	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	151	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	111	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	123	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aat
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:926635..926675 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0885 (EcoliWiki Page)	NCBI GEO profiles for aat
microarray	GenExpDB:b0885 (EcoliWiki Page)	Summary of data for aat from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aat Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11112	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1103	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0885
EcoGene	EcoGene:EG11112	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001098	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003009	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000012980 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	AAT	From SHIGELLACYC
E. coli O157	AAT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03588 Leucyl/phenylalanyl-tRNA protein transferase
Superfamily (EcoliWiki Page)	SUPERFAMILY:55729
EcoCyc	EcoCyc:EG11112	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11112	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001098	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1103	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003009	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

aat:On One Page

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Gene

Nomenclature

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Location(s) and DNA Sequence

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Sequence Features

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Alleles and Phenotypes

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Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

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Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

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Experimental

Notes

Accessions Related to aat Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

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