Tn3 bla:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

Tn3 bla

Gene Synonym(s)
Product Desc.

Penicillinase[1], Cephalosporinase[1]

Product Synonyms(s)
Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links



        

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Notes

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Gene

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Nomenclature

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Standard name

Tn3 bla

Mnemonic
Synonyms


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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

V00613: 3950..4810
<gbrowseImage> name=BM_V00613.1:3950..4810 source=Transposon_Tn3 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments
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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Tn3 beta-lactamase

Synonyms
Product description

Penicillinase[1], Cephalosporinase[1]

EC number (for enzymes)


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Notes

Certain compounds can be added to beta-lactams to inhibit beta-lactamase.

These include: Ethylenediaminetetraacetic acid (ETDA), Clavulanic acid, and 4-Chloromercuribenzoic acid (pCMB) [2]

Function

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Gene Ontology

<protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0033250

penicillinase activity

PMID:13989486[3]

IDA: Inferred from Direct Assay

F

Table 2 shows penicillinase activity in E. Coli when placed in different penicillins.

complete

GO:0033251

cephalosporinase activity

PMID:5856368[1]

IDA: Inferred from Direct Assay

F

Table 1 shows resistance to cephalosporin in E. Coli in the presence of cephalosporinase.

complete

GO:0033250

penicillinase activity

PMID:6982680[4]

IDA: Inferred from Direct Assay

F

Table 1 shows absorbance data for slight penicillinase activity in different monobactams.

complete

GO:0046677

response to antibiotic

PMID:5856368[1]

IDA: Inferred from Direct Assay

P

Table 1 shows increased Cephalosporinase and Penicillinase activity when placed in certain antibiotics.

complete

GO:0033251

cephalosporinase activity

PMID:6982680[4]

IDA: Inferred from Direct Assay

F

Table 1 shows absorbance data for slight cephalosporinase activity in different monobactams.

complete

GO:0000345

cytosolic DNA-directed RNA polymerase complex

PMID:6313485[5]

IDA: Inferred from Direct Assay

C

Figure 2 shows transposon 3 binding at a RNA Polymerase.

complete

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Interactions

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Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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Physical Properties

<protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP
EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL
CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM
PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS
RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Length

286

Mol. Wt

31.515 kDa

pI

5.9 (calculated)

Extinction coefficient

27,960 - 28,335 (calc based on 4 Y, 4 W, and 3 C residues)

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Domains/Motifs/Modification Sites

<protect> See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

AA 20-377

Beta Lactamase

Beta lactam inhibitor

UniProtID: P00811

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<motif_map/>

Structure

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Structures
Models
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Structure figures

Notes

Gene Product Resources


<protect> See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases


<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links


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Expression


Overview

Intracellular Levels


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Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)
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Notes

Transcription and Transcriptional Regulation


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Transcription unit(s)
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Notes

Translation and Regulation of Translation


Notes

Turnover and Regulation of Turnover

Notes

Experimental


Mutations Affecting Expression

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Allele Name Mutation Phenotype Reference
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Expression Studies

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Type Reference Notes
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Expression Resources

<protect> See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to Tn3_bla Expression


<protect><protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links


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Evolution


Homologs in Other Organisms


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Organism Homologs (Statistics) Comments
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Do-It-Yourself Web Tools

Notes

Families


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Database Accession Notes
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Notes

Links


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  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Ayliffe, GA (1965) Cephalosporinase and penicillinase activity of Gram-negative bacteria. J. Gen. Microbiol. 40 119-26 PubMed
  2. ↑ Bush, K et al. (1995) A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother. 39 1211-33 PubMed
  3. ↑ SMITH, JT (1963) Penicillinase and ampicillin resistance in a strain of Escherichia coli. J. Gen. Microbiol. 30 299-306 PubMed
  4. ↑ 4.0 4.1 Bush, K et al. (1982) Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria. Antimicrob. Agents Chemother. 22 414-20 PubMed
  5. ↑ Wishart, WL et al. (1983) Escherichia coli RNA polymerase binding sites and transcription initiation sites in the transposon Tn3. Gene 24 99-113 PubMed
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