Tn3 bla:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
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| Standard name |
Tn3 beta-lactamase |
|---|---|
| Synonyms | |
| Product description | |
| EC number (for enzymes) |
|
| edit table |
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Notes
Certain compounds can be added to beta-lactams to inhibit beta-lactamase.
These include: Ethylenediaminetetraacetic acid (ETDA), Clavulanic acid, and 4-Chloromercuribenzoic acid (pCMB) [2]
Function
Gene Ontology
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| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0033250 |
penicillinase activity |
IDA: Inferred from Direct Assay |
F |
Table 2 shows penicillinase activity in E. Coli when placed in different penicillins. |
complete | |||
| GO:0033251 |
cephalosporinase activity |
IDA: Inferred from Direct Assay |
F |
Table 1 shows resistance to cephalosporin in E. Coli in the presence of cephalosporinase. |
complete | |||
| GO:0033250 |
penicillinase activity |
IDA: Inferred from Direct Assay |
F |
Table 1 shows absorbance data for slight penicillinase activity in different monobactams. |
complete | |||
| GO:0046677 |
response to antibiotic |
IDA: Inferred from Direct Assay |
P |
Table 1 shows increased Cephalosporinase and Penicillinase activity when placed in certain antibiotics. |
complete | |||
| GO:0033251 |
cephalosporinase activity |
IDA: Inferred from Direct Assay |
F |
Table 1 shows absorbance data for slight cephalosporinase activity in different monobactams. |
complete | |||
| GO:0000345 |
cytosolic DNA-directed RNA polymerase complex |
IDA: Inferred from Direct Assay |
C |
Figure 2 shows transposon 3 binding at a RNA Polymerase. |
complete | |||
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Interactions
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| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
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Notes
Localization
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| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
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Notes
Structure and Physical Properties
Physical Properties
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| Name | |
|---|---|
| Sequence |
MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW |
| Length |
286 |
| Mol. Wt |
31.515 kDa |
| pI |
5.9 (calculated) |
| Extinction coefficient |
27,960 - 28,335 (calc based on 4 Y, 4 W, and 3 C residues) |
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Domains/Motifs/Modification Sites
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Structure<protect><protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.
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Structure figures<protect> | |||
Notes
Gene Product Resources
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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
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See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
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| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Ayliffe, GA (1965) Cephalosporinase and penicillinase activity of Gram-negative bacteria. J. Gen. Microbiol. 40 119-26 PubMed
- ↑ Bush, K et al. (1995) A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother. 39 1211-33 PubMed
- ↑ SMITH, JT (1963) Penicillinase and ampicillin resistance in a strain of Escherichia coli. J. Gen. Microbiol. 30 299-306 PubMed
- ↑ 4.0 4.1 Bush, K et al. (1982) Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria. Antimicrob. Agents Chemother. 22 414-20 PubMed
- ↑ Wishart, WL et al. (1983) Escherichia coli RNA polymerase binding sites and transcription initiation sites in the transposon Tn3. Gene 24 99-113 PubMed
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