Phage phiX174 E:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

E

Gene Synonym(s)

phiX174p08 [1]

Product Desc.

cell lysis protein [1]; cell lysis [1]

Product Synonyms(s)

phiX174p08 [1], gpE [1], protein E [1]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links



        

</protect> See Help:Quickview for help with entering information in the Quickview table.

Notes

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Gene

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Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

E

Mnemonic
Synonyms

phiX174p08 [1]


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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

NC_001422: 468..943
<gbrowseImage> name=NC_001422:468..943 source=bacteriophage_phiX174 preset=GeneLocation </gbrowseImage>


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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes


Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

E

Synonyms

phiX174p08 [1], gpE [1], protein E [1]

Product description

cell lysis protein [1]; cell lysis [1]

EC number (for enzymes)



Notes

Function

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Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019077

lytic viral release

PMID:19379010[2]

IDA: Inferred from Direct Assay

P

complete

GO:0005515

protein binding

PMID:19379010[2]

IPI: Inferred from Physical Interaction

EcoliWiki:mraY


F

complete

GO:0005887

integral to plasma membrane

PMID:19379010[2]

IDA: Inferred from Direct Assay

C

complete


Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes



Notes

Structure and Physical Properties

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Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence
MVRWTLWDTL AFLLLLSLLL PSLLIMFIPS TFKRPVSSWK ALNLRKTLLM ASSVRLKPLN
CSRLPCVYAQ ETLTFLLTQK KTCVKNYVRK E
Length

91

Mol. Wt

10.602 kDa

pI

10.8 (calculated)

Extinction coefficient

19,480 - 19,855 (calc based on 2 Y, 3 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

10 - 32

Predicted membrane-spanning helix.

TMD predicted by TMHMM[3]


<motif_map/>

tmhmm.php?gene=Phage_phiX174_E&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure

See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_phiX174_E taxon=10847 </beststructure>

Models

View models at:


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Structure figures

Notes

Gene Product Resources


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes


Notes

Links





Expression


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Overview

Cellular Levels


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Notes

Transcription and Transcriptional Regulation


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

|}

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Notes

Translation and Regulation of Translation


<gbrowseImage> name=NC_001422:548..588 source=bacteriophage_phiX174 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

Turnover and Regulation of Turnover

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Notes

Experimental


Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies

See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes


Expression Resources

See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes


Notes

Accessions Related to Phage phiX174 E Expression


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes


Notes

Links



Evolution


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Homologs in Other Organisms


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments


Do-It-Yourself Web Tools


Notes

Families


See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes


Notes

Links



References


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 NCBI: Enterobacteria phage phiX174, complete genome http://www.ncbi.nlm.nih.gov/nuccore/NC_001422
  2. ↑ 2.0 2.1 2.2 Zheng, Y et al. (2009) Purification and functional characterization of phiX174 lysis protein E. Biochemistry 48 4999-5006 PubMed
  3. ↑ Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed

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