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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Quickview

Standard Name	V
Gene Synonym(s)	gene V ^[1]
Product Desc.	forms dimers, DNA binding protein for efficient packaging^[2]
Product Synonyms(s)	V, protein V ^[3], unnamed protein product ^[1], GVP ^[2],GPV ^[4], G5P ^[4], single-stranded binding protein ^[4], SSBP
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links
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Notes

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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
↑ ^2.0 ^2.1 Masso, M et al. (2009) Modeling the functional consequences of single residue replacements in bacteriophage f1 gene V protein. Protein Eng. Des. Sel. 22 665-71 PubMed
↑ NCBI: Bacteriophage f1, complete genome http://www.ncbi.nlm.nih.gov/nuccore/J02448
↑ ^4.0 ^4.1 ^4.2 UniProt:P69543 (G5P_BPF1) http://www.uniprot.org/uniprot/P69543 Cite error: Invalid <ref> tag; name "uniprot:P69543" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69543" defined multiple times with different content

Gene

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Gene

Location(s) and DNA Sequence

Sequence Features

Alleles and Phenotypes

Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	V
Mnemonic
Synonyms	gene V ^[1]
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Notes

Location(s) and DNA Sequence

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<protect> <protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
		J02448: 317..1632 <gbrowseImage> name=J02448:317..1632 source=bacteriophage_f1 preset=GeneLocation </gbrowseImage>	GBrowse (Phage_f1)	FASTA Dump
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Notes

Sequence Features

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<protect> See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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<protect> See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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<protect> See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Product(s)

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Gene Product(s)

Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DNA-Binding protein G5P ^[2]
Synonyms	V, protein V ^[3], unnamed protein product ^[1], GVP ^[4],GPV ^[2], G5P ^[2], single-stranded binding protein ^[2], SSBP
Product description	forms dimers, DNA binding protein for efficient packaging^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000900	translation repressor activity, nucleic acid binding	PMID:6981459	IDA: Inferred from Direct Assay		F	Inhibits gene II mRNA	complete
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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

<protect> See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MIKVEIKPSQ AQFTTRSGVS RQGKPYSLNE QLCYVDLGNE YPVLVKITLD EGQPAYAPGL YTVHLSSFKV GQFGSLMIDR LRLVPAK
Length	87
Mol. Wt	9.687 kDa
pI	9.5 (calculated)
Extinction coefficient	7,450 - 7,575 (calc based on 5 Y, 0 W, and 1 C residues)
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Domains/Motifs/Modification Sites

<protect> See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Structure <protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_f1_V taxon=10863 </beststructure>

Models

View models at:

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Structure figures

See Help:Jmol in EcoliWiki

Gene V Protein (Single-Stranded DNA Binding Protein
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >1VQB.pdb</uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>

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Notes

Gene Product Resources

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<protect> See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
↑ ^2.0 ^2.1 ^2.2 ^2.3 UniProt:P69543 (G5P_BPF1) http://www.uniprot.org/uniprot/P69543 Cite error: Invalid <ref> tag; name "uniprot:P69543" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69543" defined multiple times with different content
↑ NCBI: Bacteriophage f1, complete genome http://www.ncbi.nlm.nih.gov/nuccore/J02448
↑ ^4.0 ^4.1 Masso, M et al. (2009) Modeling the functional consequences of single residue replacements in bacteriophage f1 gene V protein. Protein Eng. Des. Sel. 22 665-71 PubMed

Expression

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Expression

Overview

Intracellular Levels

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<protect> See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Notes

Translation and Regulation of Translation

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<gbrowseImage> name=J02448:823..863 source=bacteriophage_f1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

Turnover and Regulation of Turnover

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Notes

Experimental

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<protect> Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies <protect> See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
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Expression Resources <protect> See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to Phage_f1_V Expression

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

Evolution

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Evolution

Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

Phage f1 V:On One Page

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Notes

References

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

Expression

Overview

Intracellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to Phage_f1_V Expression

Notes

Links

References

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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