Phage f1 V:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

DNA-Binding protein G5P [1]

Synonyms

V, protein V [2], unnamed protein product [3], GVP [4],GPV [1], G5P [1], single-stranded binding protein [1], SSBP

Product description

forms dimers, DNA binding protein for efficient packaging[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000900

translation repressor activity, nucleic acid binding

PMID:6981459[5]

IDA: Inferred from Direct Assay

F

Inhibits gene II mRNA

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MIKVEIKPSQ AQFTTRSGVS RQGKPYSLNE QLCYVDLGNE YPVLVKITLD EGQPAYAPGL
YTVHLSSFKV GQFGSLMIDR LRLVPAK
Length

87

Mol. Wt

9.687 kDa

pI

9.5 (calculated)

Extinction coefficient

7,450 - 7,575 (calc based on 5 Y, 0 W, and 1 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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Structure <protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_f1_V taxon=10863 </beststructure>

Models

View models at:

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Structure figures

See Help:Jmol in EcoliWiki

Gene V Protein (Single-Stranded DNA Binding Protein
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >1VQB.pdb</uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>










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Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 UniProt:P69543 (G5P_BPF1) http://www.uniprot.org/uniprot/P69543 Cite error: Invalid <ref> tag; name "uniprot:P69543" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69543" defined multiple times with different content
  2. ↑ NCBI: Bacteriophage f1, complete genome http://www.ncbi.nlm.nih.gov/nuccore/J02448
  3. ↑ NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  4. ↑ 4.0 4.1 Masso, M et al. (2009) Modeling the functional consequences of single residue replacements in bacteriophage f1 gene V protein. Protein Eng. Des. Sel. 22 665-71 PubMed
  5. ↑ Model, P et al. (1982) The replication of bacteriophage f1: gene V protein regulates the synthesis of gene II protein. Cell 29 329-35 PubMed

Categories

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