Phage f1 I:On One Page

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions

<protect>

Standard Name

I

Gene Synonym(s)

XI

Product Desc.

I: assembly protein [1], inner membrane protein [2]


Product Synonyms(s)

protein I [3] [4], Gene I protein [2], G1P [2]

protein XI, G11P [2], I* [5]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links

porteco.png

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> <protect></protect>

Notes

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Yu, JS et al. (2007) In-frame overlapping genes: the challenges for regulating gene expression. Mol. Microbiol. 63 1158-72 PubMed
  2. 2.0 2.1 2.2 2.3 UniProt Accession P03657: http://www.uniprot.org/uniprot/P03657
  3. NCBI: Bacteriophage f1, complete genome http://www.ncbi.nlm.nih.gov/nuccore/J02448
  4. NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  5. Rapoza, MP & Webster, RE (1995) The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly. J. Mol. Biol. 248 627-38 PubMed

Gene

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

I

Mnemonic
Synonyms

XI

</protect> </protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> <protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

J02448: 3196..4242
<gbrowseImage> name=J02448:1104..6334 source=bacteriophage_f1 preset=GeneLocation </gbrowseImage>

</protect> </protect>

Notes

Sequence Features

[back to top]


<protect> See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

</protect> <protect></protect>

Notes

Alleles and Phenotypes

[back to top]


<protect> See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

</protect> <protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


<protect> See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

</protect> <protect></protect>

Notes

Accessions in Other Databases

[back to top]


<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

</protect> <protect></protect>

Notes

Links

[back to top]


<protect>

</protect>

Product(s)

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

I, XI

Synonyms

protein I [1] [2], Gene I protein [3], G1P [3]

protein XI, G11P [3], I* [4]

Product description

I: assembly protein [5], inner membrane protein [3]


EC number (for enzymes)


</protect> <protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0042963

phage assembly

PMID:7752229

IMP: Inferred from Mutant Phenotype

P

complete

GO:0016021

integral to membrane

PMID:2457024

IDA: Inferred from Direct Assay

C

complete

GO:0005887

integral to plasma membrane

PMID:1556066

IDA: Inferred from Direct Assay

C

I and XI

complete

</protect>

Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

thioredoxin, trxA

Uniprot P0AA25

Cytoplasmic domain of G1P, interacts at adhesion zone

PMID:3881414 PMID:2746731

IMP; see PMID:3881414 Table 3. Mutants with no host trxA failed to grow.

PMID:2746731 discusses interaction of thioredoxin and G1P.

DNA

Packing signal (PS) on phage DNA

Recognises PS to encapsidate phage DNA

PMID:2746731

IMP; Gene mutations promoted assembly of ss plasmid DNAs that lacked a PS into filamentous transducing particles, suggesting that the wild-type proteins may normally interact with the PS.

</protect> </protect>

Notes

Localization

<protect> See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

</protect> <protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence
MAVYFVTGKL GSGKTLVSVG KIQDKIVAGC KIATNLDLRL QNLPQVGRFA KTPRVLRIPD
KPSISDLLAI GRGNDSYDEN KNGLLVLDEC GTWFNTRSWN DKERQPIIDW FLHARKLGWD
IIFLVQDLSI VDKQARSALA ENVVYCRRLD RITLPFVGTL YSLITGSKMP LPKLHVGVVK
YGDSQLSPTV ERWLYTGKNL YNAYDTKQAF SSNYDSGVYS YLTPYLSHGR YFKPLNLGQK
MKLTKIYLKK FSRVLCLAIG FASAFTYSYI TQPKPEVKKV VSQTYDFDKF TIDSSQRLNL
SYRYVFKDSK GKLINSDDLQ KQGYSLTYID LCTVSIKKGN SNEIVKCN
Length

348

Mol. Wt

39.529 kDa

pI

9.9 (calculated)

Extinction coefficient

58,790 - 59,540 (calc based on 21 Y, 5 W, and 6 C residues)

</protect>

Domains/Motifs/Modification Sites

<protect> See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

241-295

(internal signal sequence)

  • responsible for inhibition of cell growth
  • causes loss of membrane potential
  • required for insertion in membrane

PMID:2457024

</protect>

<motif_map/>

Structure <protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_f1_I taxon=10863 </beststructure>

Models

View models at:

</protect>

</protect>

Structure figures

<protect>

</protect>

Notes

See PMID: 10547282 for info on charges of residues in alpha helix and importance to functioning of pI but not to pXI.

Gene Product Resources

[back to top]


<protect> See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

</protect> <protect></protect>

Notes

Accessions in Other Databases

[back to top]


<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

</protect> <protect></protect>

Notes

Links

[back to top]


<protect>

</protect>


References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. NCBI: Bacteriophage f1, complete genome http://www.ncbi.nlm.nih.gov/nuccore/J02448
  2. NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  3. 3.0 3.1 3.2 3.3 UniProt Accession P03657: http://www.uniprot.org/uniprot/P03657
  4. Rapoza, MP & Webster, RE (1995) The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly. J. Mol. Biol. 248 627-38 PubMed
  5. Yu, JS et al. (2007) In-frame overlapping genes: the challenges for regulating gene expression. Mol. Microbiol. 63 1158-72 PubMed

Categories

[back to top]


Expression

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

Intracellular Levels

[back to top]


<protect> See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

</protect>

Notes

Transcription and Transcriptional Regulation

[back to top]


<protect>

<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

</protect>

</protect>

Notes

Translation and Regulation of Translation

[back to top]


<gbrowseImage> name=J02448:3176..3216 source=bacteriophage_f1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

Turnover and Regulation of Turnover

</protect>

Notes

Experimental

[back to top]


<protect> Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference

</protect>

Expression Studies <protect> See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

</protect>

Expression Resources <protect> See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

</protect> <protect></protect>

Notes

Accessions Related to Phage_f1_I Expression

[back to top]


<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

</protect> <protect></protect>

Notes

Links

[back to top]


<protect>

</protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Categories

[back to top]


Evolution

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Homologs Families Links References Suggestions

Homologs in Other Organisms

[back to top]


<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

</protect>

Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

[back to top]


<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

</protect> <protect></protect>

Notes

Links

[back to top]


<protect>

</protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

Categories

[back to top]