Phage f1 I:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

I, XI

Synonyms

protein I [1] [2], Gene I protein [3], G1P [3]

protein XI, G11P [3], I* [4]

Product description

I: assembly protein [5], inner membrane protein [3]


EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0042963

phage assembly

PMID:7752229[4]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0016021

integral to membrane

PMID:2457024[6]

IDA: Inferred from Direct Assay

C

complete

GO:0005887

integral to plasma membrane

PMID:1556066[7]

IDA: Inferred from Direct Assay

C

I and XI

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

thioredoxin, trxA

Uniprot P0AA25

Cytoplasmic domain of G1P, interacts at adhesion zone

PMID:3881414[8] PMID:2746731[9]

IMP; see PMID:3881414 Table 3. Mutants with no host trxA failed to grow.

PMID:2746731 discusses interaction of thioredoxin and G1P.

DNA

Packing signal (PS) on phage DNA

Recognises PS to encapsidate phage DNA

PMID:2746731[9]

IMP; Gene mutations promoted assembly of ss plasmid DNAs that lacked a PS into filamentous transducing particles, suggesting that the wild-type proteins may normally interact with the PS.

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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MAVYFVTGKL GSGKTLVSVG KIQDKIVAGC KIATNLDLRL QNLPQVGRFA KTPRVLRIPD
KPSISDLLAI GRGNDSYDEN KNGLLVLDEC GTWFNTRSWN DKERQPIIDW FLHARKLGWD
IIFLVQDLSI VDKQARSALA ENVVYCRRLD RITLPFVGTL YSLITGSKMP LPKLHVGVVK
YGDSQLSPTV ERWLYTGKNL YNAYDTKQAF SSNYDSGVYS YLTPYLSHGR YFKPLNLGQK
MKLTKIYLKK FSRVLCLAIG FASAFTYSYI TQPKPEVKKV VSQTYDFDKF TIDSSQRLNL
SYRYVFKDSK GKLINSDDLQ KQGYSLTYID LCTVSIKKGN SNEIVKCN
Length

348

Mol. Wt

39.529 kDa

pI

9.9 (calculated)

Extinction coefficient

58,790 - 59,540 (calc based on 21 Y, 5 W, and 6 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References

Domain

241-295

(internal signal sequence)

  • responsible for inhibition of cell growth
  • causes loss of membrane potential
  • required for insertion in membrane

PMID:2457024[6]

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Structure <protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_f1_I taxon=10863 </beststructure>

Models

View models at:

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Structure figures

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Notes

See PMID: 10547282 for info on charges of residues in alpha helix and importance to functioning of pI but not to pXI.

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ NCBI: Bacteriophage f1, complete genome http://www.ncbi.nlm.nih.gov/nuccore/J02448
  2. ↑ NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  3. ↑ 3.0 3.1 3.2 3.3 UniProt Accession P03657: http://www.uniprot.org/uniprot/P03657
  4. ↑ 4.0 4.1 Rapoza, MP & Webster, RE (1995) The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly. J. Mol. Biol. 248 627-38 PubMed
  5. ↑ Yu, JS et al. (2007) In-frame overlapping genes: the challenges for regulating gene expression. Mol. Microbiol. 63 1158-72 PubMed
  6. ↑ 6.0 6.1 Horabin, JI & Webster, RE (1988) An amino acid sequence which directs membrane insertion causes loss of membrane potential. J. Biol. Chem. 263 11575-83 PubMed
  7. ↑ Guy-Caffey, JK et al. (1992) Membrane localization and topology of a viral assembly protein. J. Bacteriol. 174 2460-5 PubMed
  8. ↑ Lim, CJ et al. (1985) Thioredoxin is the bacterial protein encoded by fip that is required for filamentous bacteriophage f1 assembly. J. Bacteriol. 161 799-802 PubMed
  9. ↑ 9.0 9.1 Russel, M & Model, P (1989) Genetic analysis of the filamentous bacteriophage packaging signal and of the proteins that interact with it. J. Virol. 63 3284-95 PubMed

Categories

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