Phage T7 4:On One Page
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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| Standard Name |
4 |
|---|---|
| Gene Synonym(s) |
Phage_T7_4A, |
| Product Desc. |
primase/helicase [14,15] |
| Product Synonyms(s) |
Phage_T7_4A, |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links |
|
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</protect></protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
4 |
|---|---|
| Mnemonic | |
| Synonyms |
Phage_T7_4A, |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
NC_1604: 11466..13366 |
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Notes
Sequence Features
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ATG to TTA at codon 64 |
M64L |
PMID:1321823 |
aka 4A' | ||||
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Notes
Plasmids expressing gene 4 can be used to complement growth of 4 deletion phage[1]. Versions of these plasmids that express only 4A (M64L) support growth of T7 4 deletions.
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
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| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
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Notes
Accessions in Other Databases
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See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Notes
Links
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| Name | URL | Comments |
|---|---|---|
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Product(s)
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Nomenclature
<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
4A |
|---|---|
| Synonyms |
Phage_T7_4A, |
| Product description |
primase/helicase [14,15] |
| EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
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See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
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Interactions
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| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
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Notes
Localization
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| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
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See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | ||
|---|---|---|
| Sequence |
MDNSHDSDSV FLYHIPCDNC GSSDGNSLFS DGHTFCYVCE KWTAGNEDTK ERASKRKPSG GKPMTYNVWN FGESNGRYSA LTARGISKET CQKAGYWIAK VDGVMYQVAD YRDQNGNIVS QKVRDKDKNF KTTGSHKSDA LFGKHLWNGG KKIVVTEGEI DMLTVMELQD CKYPVVSLGH GASAAKKTCA ANYEYFDQFE QIILMFDMDE AGRKAVEEAA QVLPAGKVRV AVLPCKDANE CHLNGHDREI MEQVWNAGPW IPDGVVSALS LRERIREHLS SEESVGLLFS GCTGINDKTL GARGGEVIMV TSGSGMGKST FVRQQALQWG TAMGKKVGLA MLEESVEETA EDLIGLHNRV RLRQSDSLKR EIIENGKFDQ WFDELFGNDT FHLYDSFAEA ETDRLLAKLA YMRSGLGCDV IILDHISIVV SASGESDERK MIDNLMTKLK GFAKSTGVVL VVICHLKNPD KGKAHEEGRP VSITDLRGSG ALRQLSDTII ALERNQQGDM PNLVLVRILK CRFTGDTGIA GYMEYNKETG WLEPSSYSGE EESHSESTDW SNDTDF |
MTYNVWNFGE SNGRYSALTA RGISKETCQK AGYWIAKVDG VMYQVADYRD QNGNIVSQKV RDKDKNFKTT GSHKSDALFG KHLWNGGKKI VVTEGEIDML TVMELQDCKY PVVSLGHGAS AAKKTCAANY EYFDQFEQII LMFDMDEAGR KAVEEAAQVL PAGKVRVAVL PCKDANECHL NGHDREIMEQ VWNAGPWIPD GVVSALSLRE RIREHLSSEE SVGLLFSGCT GINDKTLGAR GGEVIMVTSG SGMGKSTFVR QQALQWGTAM GKKVGLAMLE ESVEETAEDL IGLHNRVRLR QSDSLKREII ENGKFDQWFD ELFGNDTFHL YDSFAEAETD RLLAKLAYMR SGLGCDVIIL DHISIVVSAS GESDERKMID NLMTKLKGFA KSTGVVLVVI CHLKNPDKGK AHEEGRPVSI TDLRGSGALR QLSDTIIALE RNQQGDMPNL VLVRILKCRF TGDTGIAGYM EYNKETGWLE PSSYSGEEES HSESTDWSND TDF |
| Length |
566 |
503 |
| Mol. Wt |
62.655 kDa |
55.743 kDa |
| pI |
5.3 (calculated) |
5.3 (calculated) |
| Extinction coefficient |
77,350 - 78,975 (calc based on 15 Y, 10 W, and 13 C residues) |
68,870 - 69,995 (calc based on 13 Y, 9 W, and 9 C residues) |
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Domains/Motifs/Modification Sites
| <protect>
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|
<motif_map/> |
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Structure
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Structure figures<protect> | ||||||
Notes
Gene 4 encodes both the 4A and 4B products. The shorter 4B protein is from an internal initiation site. 4A has helicase and primase activities, while 4B has primase activity only. [2]
Gene Product Resources
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| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
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See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
| edit table |
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Notes
Links
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| Name | URL | Comments |
|---|---|---|
| edit table |
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Expression
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Overview
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
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Notes
Transcription and Transcriptional Regulation
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Notes
Translation and Regulation of Translation
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name=NC_001604:11546..11586
source=bacteriophage_T7
type=Gene+DNA_+Protein
preset=Nterminus
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This picture shows the sequence around the N-terminus.
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Notes
Turnover and Regulation of Turnover
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Notes
Experimental
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Mutations Affecting Expression
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See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
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Expression Studies
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| Type | Reference | Notes |
|---|---|---|
| edit table |
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Expression Resources
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| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
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Notes
Accessions Related to Phage T7 4A Expression
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| Database | Accession | Notes |
|---|---|---|
| edit table |
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Notes
Links
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| Name | URL | Comments |
|---|---|---|
| edit table |
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Evolution
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Homologs in Other Organisms
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| Organism | Homologs (Statistics) | Comments |
|---|---|---|
| edit table |
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Do-It-Yourself Web Tools
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Notes
Families
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See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.
| Database | Accession | Notes |
|---|---|---|
| edit table |
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Notes
Links
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| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Rosenberg, AH et al. (1992) Cloning and expression of gene 4 of bacteriophage T7 and creation and analysis of T7 mutants lacking the 4A primase/helicase or the 4B helicase. J. Biol. Chem. 267 15005-12 PubMed
- ↑ Bernstein, JA & Richardson, CC (1988) A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity. Proc. Natl. Acad. Sci. U.S.A. 85 396-400 PubMed
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