Phage T7 4:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

4A

Synonyms

Phage_T7_4A,

Product description

primase/helicase [14,15]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MDNSHDSDSV FLYHIPCDNC GSSDGNSLFS DGHTFCYVCE KWTAGNEDTK ERASKRKPSG
GKPMTYNVWN FGESNGRYSA LTARGISKET CQKAGYWIAK VDGVMYQVAD YRDQNGNIVS
QKVRDKDKNF KTTGSHKSDA LFGKHLWNGG KKIVVTEGEI DMLTVMELQD CKYPVVSLGH
GASAAKKTCA ANYEYFDQFE QIILMFDMDE AGRKAVEEAA QVLPAGKVRV AVLPCKDANE
CHLNGHDREI MEQVWNAGPW IPDGVVSALS LRERIREHLS SEESVGLLFS GCTGINDKTL
GARGGEVIMV TSGSGMGKST FVRQQALQWG TAMGKKVGLA MLEESVEETA EDLIGLHNRV
RLRQSDSLKR EIIENGKFDQ WFDELFGNDT FHLYDSFAEA ETDRLLAKLA YMRSGLGCDV
IILDHISIVV SASGESDERK MIDNLMTKLK GFAKSTGVVL VVICHLKNPD KGKAHEEGRP
VSITDLRGSG ALRQLSDTII ALERNQQGDM PNLVLVRILK CRFTGDTGIA GYMEYNKETG
WLEPSSYSGE EESHSESTDW SNDTDF
 
MTYNVWNFGE SNGRYSALTA RGISKETCQK AGYWIAKVDG VMYQVADYRD QNGNIVSQKV
RDKDKNFKTT GSHKSDALFG KHLWNGGKKI VVTEGEIDML TVMELQDCKY PVVSLGHGAS
AAKKTCAANY EYFDQFEQII LMFDMDEAGR KAVEEAAQVL PAGKVRVAVL PCKDANECHL
NGHDREIMEQ VWNAGPWIPD GVVSALSLRE RIREHLSSEE SVGLLFSGCT GINDKTLGAR
GGEVIMVTSG SGMGKSTFVR QQALQWGTAM GKKVGLAMLE ESVEETAEDL IGLHNRVRLR
QSDSLKREII ENGKFDQWFD ELFGNDTFHL YDSFAEAETD RLLAKLAYMR SGLGCDVIIL
DHISIVVSAS GESDERKMID NLMTKLKGFA KSTGVVLVVI CHLKNPDKGK AHEEGRPVSI
TDLRGSGALR QLSDTIIALE RNQQGDMPNL VLVRILKCRF TGDTGIAGYM EYNKETGWLE
PSSYSGEEES HSESTDWSND TDF
Length

566

503

Mol. Wt

62.655 kDa

55.743 kDa

pI

5.3 (calculated)

5.3 (calculated)

Extinction coefficient

77,350 - 78,975 (calc based on 15 Y, 10 W, and 13 C residues)

68,870 - 69,995 (calc based on 13 Y, 9 W, and 9 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References

Domain

281 - 548

SF4 helicase

UniProt:P03692


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Structure
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Structures

<beststructure> gene=Phage_T7_4A taxon=10760 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene 4 encodes both the 4A and 4B products. The shorter 4B protein is from an internal initiation site. 4A has helicase and primase activities, while 4B has primase activity only. [1]

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Bernstein, JA & Richardson, CC (1988) A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity. Proc. Natl. Acad. Sci. U.S.A. 85 396-400 PubMed

Categories

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