Phage P1 darB:On One Page

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Standard Name	darB
Gene Synonym(s)
Product Desc.	defense against restriction; putative DNA adenine methyltransferase and helicase; protects against a subset of type I enzymes [EcoB, EcoK], head structural protein; similar to putative 2231 aa protein of Helicibacter pyroli in GenBank Accession Number AE001522 and to putative 2849 aa DNA methylase of Sinorhizobium meliloti phage PBC5 in GenBank Accession Number NP_542283;
Product Synonyms(s)	gp011
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links
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Notes

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Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	darB
Mnemonic
Synonyms
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Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
		NC_005856: 0..30770 <gbrowseImage> name=NC_005856:0..30770 source=bacteriophage_P1 preset=GeneLocation </gbrowseImage>	GBrowse (Phage_P1)	FASTA Dump
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Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
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Accessions in Other Databases

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Database	Accession	Notes
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Links

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Name	URL	Comments
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References

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Product(s)

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Sequence

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DarB
Synonyms	gp011
Product description	defense against restriction; putative DNA adenine methyltransferase and helicase; protects against a subset of type I enzymes [EcoB, EcoK], head structural protein; similar to putative 2231 aa protein of Helicibacter pyroli in GenBank Accession Number AE001522 and to putative 2849 aa DNA methylase of Sinorhizobium meliloti phage PBC5 in GenBank Accession Number NP_542283;
EC number (for enzymes)
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Function

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Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	PMID:3029954	IDA: Inferred from Direct Assay		F	Page 164	complete
	GO:0008170	N-methyltransferase activity	PMID:15489417	IDA: Inferred from Direct Assay		F	Fig 5, Supplemental Table 1	complete
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Interactions

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Partner Type	Partner	Notes	References	Evidence
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Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MNKLSMGVFR CSSVSEILKY IRAITSHRAP IKYGVEKVEG KSYDRLRREA NQKAIDLLNS LVDGATLTDE QRQILAGYTG EGGIGGSVSE YYTPKPIAEG VWEIMKLYGA DVGNTLEPSA GTGVFNETKP VGTVMTATEI SSVSGRINQL LHPEDSVQIS PFEQLAVSTP NDSFDHVVGN VPFGGRDNTR NIDKPYAEET DMGSYFMLRM LDKIKPGGFM CVIVPPSIVS GSNMKRLRLR LSRKAEFLGA HRLPTGTFDA NGTSTVVDVV LMRKHPAEMA EKIPLVHEST LESANVLWPT FISGKWFEKD GRRFVHGTQE KGFQGRIEVR ADGQIDNQAL KAKLIHCFES RIDWYLLDMA EPSPTADVVD EGEMRLINGV WQKYAGGRWI ESDAGKELKI DVASYGADSW EALQRNLTTT EGRLGMTFTQ MANVRDKYTT SISDDMVQLV DWINSQPEKY RERLYRGAMI GRMLIEYQDM KAAGHSAEQI EQQRLSLVSR LQAEIDRFGN PGRGPIAKLS GSGARAWFAF RGAIKLDGTI SDELTGKLVT HDSSASYDST SYQDTLRYLY SDLTRDPIQL DDFRLAFTGE LPASDDELLN LLASTPGIAV SPYGGIVPFA RATSGDINEI VAPKQEFLAT LTDGPVKNNV LNQLAAIEEK RIKTPAENIR FKLNSRWFDR SVILEFLQEN GYPDLRYVQS VQLEGDEMVS DTYHGGDGLF VGHRYGVVQR KDKETGEIRY EWDRKSGENA TGFPAQLEKY LNGARIGGKD SATANGYREQ MALLEDQFNK WIKTHDRYDE LVAKYNDVFN SNIPYEHSGD PLGLKGLSGK RQPFDYQNSE VRRLSEDGRG ILGFGTGLGK TTTALALEAF NYENGRSTRT AYVVPKSVLE NWYYEAKEFL SEEAFSNYLF VGLDVLMDGD QIRQVPVLDE NGKPVLGTDG TPVMRDALKL ADEATITARM NAIPHSNYRA VVFTKEQYAR IPLRDDTVDE HAQDMLYDFV AAGRVASAMD SDSHRKEAAR RRVLSEYSDT GTEKAEKYPY FEDMGFDSVI ADEGHNYRNS YKNGREASQL AYLPTSAVAQ SARDMAIKNA YLMKKNGGRG PVLLTATPVV NTPIDAYNML SHVLPKEYWQ KMGIYGPDDF VKFFGKTRLE TVQKISGEVE EKMALVGFEN LDALRGIFHR WTTLKTAEDV KDTVEIPELD EHQQDAPLTE EQLAAYEELR QQAEAAAKAN NGVTTSVNED GVIEHEKARP IFSIIRDMDR VCTDMDLYYR RITYRFLPEY ADAVQQLADS LPKQATSEDD DSDDSITQQS QYSLIDKGEF IQLQVPEAFE QEVNKRLARF GIDEQTVTHP VTPKYAKLIA TLKEFFPEGK QIIFTDEKTQ HQKLKRIICN ALNLEPSKVG ILNAQTVAEA GKTGKKLKAV KPPKELPDEP TDAQIAKYNE QMALYDAYIA QQNEMSLGGL EKIAADFQEG RTPIIICNKK AEVGINLHRG TTDIHHLTLP WTPASIAQRN GRGARVGSNR ASVRVHYYCG KGSFDEYRLK TLKRKAGWIS DILRSDKSEM ENADANDMIE MQMYTAKDDG ERLAMMQVQM DKAKAAQRAR QKEQATIDLQ NYIKAQHAAG EDVEVLTAEL ERSKAELEKT TAEVAKFKQA VMAKAADNAD WKARWGSVHH TDRMLLAQYR ASLKSAIQRK ANISQAISPY EKLLNRTQKA ATDIKRLRPL VEDAINKGIL DVDPDLVNHA SEFLVIGDRS WRVGQYYDCA GDIVRIKSLD FDSQRADVEI IFTFKGTKSG NWDVKTLDKQ VDVTPDEDAV MQKISGGVSI AGINDIISCD DFYRFQQRGM IKITDSYGVQ TTESGYSIDF VGTYTDPLKH AVYPDRRDGA LKSSIAKWVL GMMSEGNNRQ VRLAEVFLTE LFGSNYGDVI ASYGDTLSPE AIQEKIADAI ARMPEKTSQG ATRNGDSELE VTNAIFGTHE FRASDYEITT AQFGTIGIYS NKAEIKQAMD AASARIAAER EANLNHAVAA LTQSWVTAIR EAATTGKITP AIADVVNDGS KFMDAYKMDA VQLPSAYGQL SYRMTYNLVS MFSDLAILGL VDLNEVTPEL LSMRKNHVEI LQRINTVLAG RTDEEKQADA DRINLALGNI TEEEIAARNE KQEELSSIQG DATSIAQSLG LNYRVSTADL KMMYAPKFAA GEVFGLQEAS GMKGVLFRAK DAIKTKFGAR WLPAKAKNSD FPGNWWIIET KHNVADVLAV IQQYA
Length	2,255
Mol. Wt	251.548 kDa
pI	5.4 (calculated)
Extinction coefficient	269,650 - 270,775 (calc based on 85 Y, 26 W, and 9 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki. Type Residues Description Notes References edit table

<motif_map/>

Structure See Help:Product_structure for help entering or editing information in this section of EcoliWiki. Structures <beststructure> gene=Phage_P1_darB taxon=10678 </beststructure> Models View models at: PSI-PRED Genomes TO Protein (GTOP) edit table

</protect> Structure figures Notes Gene Product Resources [back to top] See Help:Product_resources for help with entering or editing information in this section of EcoliWiki. Resource type Source Notes/Reference edit table Notes Accessions in Other Databases [back to top] See Help:Gene_accessions for help with entering information into the Gene Accessions table. Database Accession Notes edit table Notes Links [back to top] Name URL Comments edit table References [back to top] See Help:References for how to manage references in EcoliWiki. Categories [back to top] Expression [back to top] {{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page     Overview Transcription Translation Turnover Experimental Links References Suggestions Overview Cellular Levels [back to top] See Help:Cellular levels Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s) edit table Notes Transcription and Transcriptional Regulation [back to top] <protect> See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki. Transcription unit(s) edit table |} </protect> Notes Translation and Regulation of Translation [back to top] <gbrowseImage> name=NC_005856:17216..17256 source=bacteriophage_P1 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus. Notes Turnover and Regulation of Turnover </protect> Notes Experimental [back to top] Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki. Allele Name Mutation Phenotype Reference edit table Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki. Type Reference Notes edit table Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki. Resource Name Resource Type Description Source Notes edit table Notes Accessions Related to Phage_P1_darB Expression [back to top] See Help:Gene_accessions for help with entering information into the Gene Accessions table. Database Accession Notes edit table Notes Links [back to top] Name URL Comments edit table References [back to top] See Help:References for how to manage references in EcoliWiki. Categories [back to top] Evolution [back to top] {{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page     Homologs Families Links References Suggestions Homologs in Other Organisms [back to top] See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki. Organism Homologs (Statistics) Comments edit table Do-It-Yourself Web Tools BLAST CDD InParanoid YOGY Notes Families [back to top] See Help:Evolution_families for help entering or editing information in this section of EcoliWiki. Database Accession Notes edit table Notes Links [back to top] Name URL Comments edit table References [back to top] See Help:References for how to manage references in EcoliWiki. Categories [back to top]