Phage P1 darB:Gene Product(s)

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DarB

Synonyms

gp011

Product description

defense against restriction; putative DNA adenine methyltransferase and helicase; protects against a subset of type I enzymes [EcoB, EcoK], head structural protein; similar to putative 2231 aa protein of Helicibacter pyroli in GenBank Accession Number AE001522 and to putative 2849 aa DNA methylase of Sinorhizobium meliloti phage PBC5 in GenBank Accession Number NP_542283;

EC number (for enzymes)


</protect> <protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

PMID:3029954[1]

IDA: Inferred from Direct Assay

F

Page 164

complete

GO:0008170

N-methyltransferase activity

PMID:15489417[2]

IDA: Inferred from Direct Assay

F

Fig 5, Supplemental Table 1

complete

</protect>

Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

</protect> </protect>

Notes

Localization

<protect> See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

</protect> <protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence
MNKLSMGVFR CSSVSEILKY IRAITSHRAP IKYGVEKVEG KSYDRLRREA NQKAIDLLNS
LVDGATLTDE QRQILAGYTG EGGIGGSVSE YYTPKPIAEG VWEIMKLYGA DVGNTLEPSA
GTGVFNETKP VGTVMTATEI SSVSGRINQL LHPEDSVQIS PFEQLAVSTP NDSFDHVVGN
VPFGGRDNTR NIDKPYAEET DMGSYFMLRM LDKIKPGGFM CVIVPPSIVS GSNMKRLRLR
LSRKAEFLGA HRLPTGTFDA NGTSTVVDVV LMRKHPAEMA EKIPLVHEST LESANVLWPT
FISGKWFEKD GRRFVHGTQE KGFQGRIEVR ADGQIDNQAL KAKLIHCFES RIDWYLLDMA
EPSPTADVVD EGEMRLINGV WQKYAGGRWI ESDAGKELKI DVASYGADSW EALQRNLTTT
EGRLGMTFTQ MANVRDKYTT SISDDMVQLV DWINSQPEKY RERLYRGAMI GRMLIEYQDM
KAAGHSAEQI EQQRLSLVSR LQAEIDRFGN PGRGPIAKLS GSGARAWFAF RGAIKLDGTI
SDELTGKLVT HDSSASYDST SYQDTLRYLY SDLTRDPIQL DDFRLAFTGE LPASDDELLN
LLASTPGIAV SPYGGIVPFA RATSGDINEI VAPKQEFLAT LTDGPVKNNV LNQLAAIEEK
RIKTPAENIR FKLNSRWFDR SVILEFLQEN GYPDLRYVQS VQLEGDEMVS DTYHGGDGLF
VGHRYGVVQR KDKETGEIRY EWDRKSGENA TGFPAQLEKY LNGARIGGKD SATANGYREQ
MALLEDQFNK WIKTHDRYDE LVAKYNDVFN SNIPYEHSGD PLGLKGLSGK RQPFDYQNSE
VRRLSEDGRG ILGFGTGLGK TTTALALEAF NYENGRSTRT AYVVPKSVLE NWYYEAKEFL
SEEAFSNYLF VGLDVLMDGD QIRQVPVLDE NGKPVLGTDG TPVMRDALKL ADEATITARM
NAIPHSNYRA VVFTKEQYAR IPLRDDTVDE HAQDMLYDFV AAGRVASAMD SDSHRKEAAR
RRVLSEYSDT GTEKAEKYPY FEDMGFDSVI ADEGHNYRNS YKNGREASQL AYLPTSAVAQ
SARDMAIKNA YLMKKNGGRG PVLLTATPVV NTPIDAYNML SHVLPKEYWQ KMGIYGPDDF
VKFFGKTRLE TVQKISGEVE EKMALVGFEN LDALRGIFHR WTTLKTAEDV KDTVEIPELD
EHQQDAPLTE EQLAAYEELR QQAEAAAKAN NGVTTSVNED GVIEHEKARP IFSIIRDMDR
VCTDMDLYYR RITYRFLPEY ADAVQQLADS LPKQATSEDD DSDDSITQQS QYSLIDKGEF
IQLQVPEAFE QEVNKRLARF GIDEQTVTHP VTPKYAKLIA TLKEFFPEGK QIIFTDEKTQ
HQKLKRIICN ALNLEPSKVG ILNAQTVAEA GKTGKKLKAV KPPKELPDEP TDAQIAKYNE
QMALYDAYIA QQNEMSLGGL EKIAADFQEG RTPIIICNKK AEVGINLHRG TTDIHHLTLP
WTPASIAQRN GRGARVGSNR ASVRVHYYCG KGSFDEYRLK TLKRKAGWIS DILRSDKSEM
ENADANDMIE MQMYTAKDDG ERLAMMQVQM DKAKAAQRAR QKEQATIDLQ NYIKAQHAAG
EDVEVLTAEL ERSKAELEKT TAEVAKFKQA VMAKAADNAD WKARWGSVHH TDRMLLAQYR
ASLKSAIQRK ANISQAISPY EKLLNRTQKA ATDIKRLRPL VEDAINKGIL DVDPDLVNHA
SEFLVIGDRS WRVGQYYDCA GDIVRIKSLD FDSQRADVEI IFTFKGTKSG NWDVKTLDKQ
VDVTPDEDAV MQKISGGVSI AGINDIISCD DFYRFQQRGM IKITDSYGVQ TTESGYSIDF
VGTYTDPLKH AVYPDRRDGA LKSSIAKWVL GMMSEGNNRQ VRLAEVFLTE LFGSNYGDVI
ASYGDTLSPE AIQEKIADAI ARMPEKTSQG ATRNGDSELE VTNAIFGTHE FRASDYEITT
AQFGTIGIYS NKAEIKQAMD AASARIAAER EANLNHAVAA LTQSWVTAIR EAATTGKITP
AIADVVNDGS KFMDAYKMDA VQLPSAYGQL SYRMTYNLVS MFSDLAILGL VDLNEVTPEL
LSMRKNHVEI LQRINTVLAG RTDEEKQADA DRINLALGNI TEEEIAARNE KQEELSSIQG
DATSIAQSLG LNYRVSTADL KMMYAPKFAA GEVFGLQEAS GMKGVLFRAK DAIKTKFGAR
WLPAKAKNSD FPGNWWIIET KHNVADVLAV IQQYA
Length

2,255

Mol. Wt

251.548 kDa

pI

5.4 (calculated)

Extinction coefficient

269,650 - 270,775 (calc based on 85 Y, 26 W, and 9 C residues)

</protect>

Domains/Motifs/Modification Sites

<protect> See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

</protect>

<motif_map/>

Structure
<protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_P1_darB taxon=10678 </beststructure>

Models

View models at:

</protect>

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


<protect> See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

</protect> <protect></protect>

Notes

Accessions in Other Databases

[back to top]


<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

</protect> <protect></protect>

Notes

Links

[back to top]


<protect>

</protect>


References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Iida, S et al. (1987) Two DNA antirestriction systems of bacteriophage P1, darA, and darB: characterization of darA- phages. Virology 157 156-66 PubMed
  2. Łobocka, MB et al. (2004) Genome of bacteriophage P1. J. Bacteriol. 186 7032-68 PubMed

Categories

[back to top]