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Gene |

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Standard Name	50
Gene Synonym(s)	gp50 ^[1], EPNV4_gp50 ^[1]
Product Desc.	virion RNA polymerase ^[1]
Product Synonyms(s)	gp50 ^[1], EPNV4_gp50 ^[1]
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
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Notes

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Gene

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Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	50
Mnemonic
Synonyms	gp50 ^[1], EPNV4_gp50 ^[1]
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Notes

Location(s) and DNA Sequence

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<protect> <protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
		NC_8720: 45125..34423 <gbrowseImage> name=NC_008720:34423..45125 source=bacteriophage_N4 preset=GeneLocation </gbrowseImage>	GBrowse(Bacteriophage N4)	FASTA Dump
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Notes

Sequence Features

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<protect> See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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<protect> See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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<protect> See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	50
Synonyms	gp50 ^[1], EPNV4_gp50 ^[1]
Product description	virion RNA polymerase ^[1]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003899	DNA-directed RNA polymerase activity	PMID:6989837	IDA: Inferred from Direct Assay		F		complete
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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

<protect> See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MSVFDRLAGF ADSVTNAKQV DVSTATAQKK AEQGVTTPLV SPDAAYQMQA ARTGNVGANA FEPGTVQSDF MNLTPMQIMN KYGVEQGLQL INARADAGNQ VFNDSVTTRT PGEELGDIAT GVGLGFVNTL GGIGALGAGL LNDDAGAVVA QQLSKFNDAV HATQSQALQD KRKLFAARNL MNEVESERQY QTDKKEGTND IVASLSKFGR DFVGSIENAA QTDSIISDGL AEGVGSLLGA GPVLRGASLL GKAVVPANTL RSAALAGAID AGTGTQSLAR IASTVGRAAP GMVGVGAMEA GGAYQQTADE IMKMSLKDLE KSPVYQQHIK DGMSPEQARR QTASETGLTA AAIQLPIAAA TGPLVSRFEM APFRAGSLGA VGMNLARETV EEGVQGATGQ LAQNIAQQQN IDKNQDLLKG VGTQAGLGAL YGFGSAGVVQ APAGAARLAG AATAPVLRTT MAGVKAAGSV AGKVVSPIKN TLVARGERVM KQNEEASPVA DDYVAQAAQE AMAQAPEAEV TIRDAVEATD ATPEQKVAAH QYVSDLMNAT RFNPENYQEA PEHIRNAVAG STDQVQVIQK LADLVNTLDE SNPQALMEAA SYMYDAVSEF EQFINRDPAA LDSIPKDSPA IELLNRYTNL TANIQNTPKV IGALNVINRM INESAQNGSL NVTEESSPQE MQNVALAAEV APEKLNPESV NVVLKHAADG RIKLNNRQIA ALQNAAAILK GAREYDAEAA RLGLRPQDIV SKQIKTDESR TQEGQYSALQ HANRIRSAYN SGNFELASAY LNDFMQFAQH MQNKVGALNE HLVTGNADKN KSVHYQALTA DREWVRSRTG LGVNPYDTKS VKFAQQVALE AKTVADIANA LASAYPELKV SHIKVTPLDS RLNAPAAEVV KAFRQGNRDV ASSQPKADSV NQVKETPVTK QEPVTSTVQT KTPVSESVKT EPTTKESSPQ AIKEPVNQSE KQDVNLTNED NIKQPTESVK ETETSTKEST VTEELKEGID AVYPSLVGTA DSKAEGIKNY FKLSFTLPEE QKSRTVGSEA PLKDVAQALS SRARYELFTE KETANPAFNG EVIKRYKELM EHGEGIADIL RSRLAKFLNT KDVGKRFAQG TEANRWVGGK LLNIVEQDGD TFKYNEQLLQ TAVLAGLQWR LTATSNTAIK DAKDVAAITG IDQALLPEGL VEQFDTGMTL TEAVSSLAQK IESYWGLSRN PNAPLGYTKG IPTAMAAEIL AAFVESTDVV ENIVDMSEID PDNKKTIGLY TITELDSFDP INSFPTAIEE AVLVNPTEKM FFGDDIPPVA NTQLRNPAVR NTPEQKAALK AEQATEFYVH TPMVQFYETL GKDRILELMG AGTLNKELLN DNHAKSLEGK NRSVEDSYNQ LFSVIEQVRA QSEDISTVPI HYAYNMTRVG RMQMLGKYNP QSAKLVREAI LPTKATLDLS NQNNEDFSAF QLGLAQALDI KVHTMTREVM SDELTKLLEG NLKPAIDMMV EFNTTGSLPE NAVDVLNTAL GDRKSFVALM ALMEYSRYLV AEDKSAFVTP LYVEADGVTN GPINAMMLMT GGLFTPDWIR NIAKGGLFIG SPNKTMNEHR STADNNDLYQ ASTNALMESL GKLRSNYASN MPIQSQIDSL LSLMDLFLPD INLGENGALE LKRGIAKNPL TITIYGSGAR GIAGKLVSSV TDAIYERMSD VLKARAKDPN ISAAMAMFGK QAASEAHAEE LLARFLKDME TLTSTVPVKR KGVLELQSTG TGAKGKINPK TYTIKGEQLK ALQENMLHFF VEPLRNGITQ TVGESLVYST EQLQKATQIQ SVVLEDMFKQ RVQEKLAEKA KDPTWKKGDF LTQKELNDIQ ASLNNLAPMI ETGSQTFYIA GSENAEVANQ VLATNLDDRM RVPMSIYAPA QAGVAGIPFM TIGTGDGMMM QTLSTMKGAP KNTLKIFDGM NIGLNDITDA SRKANEAVYT SWQGNPIKNV YESYAKFMKN VDFSKLSPEA LEAIGKSALE YDQRENATVD DIANAASLIE RNLRNIALGV DIRHKVLDKV NLSIDQMAAV GAPYQNNGKI DLSNMTPEQQ ADELNKLFRE ELEARKQKVA KARAEVKEET VSEKEPVNPD FGMVGREHKA SGVRILSATA IRNLAKISNL PSTQAATLAE IQKSLAAKDY KIIYGTPTQV AEYARQKNVT ELTSQEMEEA QAGNIYGWTN FDDKTIYLVS PSMETLIHEL VHASTFEEVY SFYQGNEVSP TSKQAIENLE GLMEQFRSLD ISKDSPEMRE AYADAIATIE GHLSNGFVDP AISKAAALNE FMAWGLANRA LAAKQKRTSS LVQMVKDVYQ AIKKLIWGRK QAPALGEDMF SNLLFNSAIL MRSQPTTQAV AKDGTLFHSK AYGNNERLSQ LNQTFDKLVT DYLRTDPVTE VERRGNVANA LMSATRLVRD VQSHGFNMTA QEQSVFQMVT AALATEAAID PHAMARAQEL YTHVMKHLTV EHFMADPDST NPADRYYAQQ KYDTISGANL VEVDAKGRTS LLPTFLGLAM VNEELRSIIK EMPVPKADKK LGNDIDTLLT NAGTQVMESL NRRMAGDQKA TNVQDSIDAL SETIMAAALK RESFYDAVAT PTGNFIDRAN QYVTDSIERL SETVIEKADK VIANPSNIAA KGVAHLAKLT AAIASEKQGE IVAQGVMTAM NQGKVWQPFH DLVNDIVGRT KTNANVYDLI KLVKSQISQD RQQFREHLPT VIAGKFSRKL TDTEWSAMHT GLGKTDLAVL RETMSMAEIR DLLSSSKKVK DEISTLEKEI QNQAGRNWNL VQKKSKQLAQ YMIMGEVGNN LLRNAHAISR LLGERITNGP VADVAAIDKL ITLYSLELMN KSDRDLLSEL AQSEVEGMEF SIAYMVGQRT EEMRKAKGDN RTLLNHFKGY IPVENQQGVN LIIADDKEFA KLNSQSFTRI GTYQGSTGFR TGSKGYYFSP VAARAPYSQG ILQNVRNTAG GVDIGTGFTL GTMVAGRITD KPTVERITKA LAKGERGREP LMPIYNSKGQ VVAYEQSVDP NMLKHLNQDN HFAKMVGVWR GRQVEEAKAQ RFNDILIEQL HAMYEKDIKD SSANKSQYVN LLGKIDDPVL ADAINLMNIE TRHKAEELFG KDELWVRRDM LNDALGYRAA SIGDVWTGNS RWSPSTLDTV KKMFLGAFGN KAYHVVMNAE NTIQNLVKDA KTVIVVKSVV VPAVNFLANI YQMIGRGVPV KDIAVNIPRK TSEINQYIKS RLRQIDAEAE LRAAEGNPNL VRKLKTEIQS ITDSHRRMSI WPLIEAGEFS SIADAGISRD DLLVAEGKIH EYMEKLANKL PEKVRNAGRY ALIAKDTALF QGIQKTVEYS DFIAKAIIYD DLVKRKKKSS SEALGQVTEE FINYDRLPGR
Length	3,410
Mol. Wt	372.322 kDa
pI	5.7 (calculated)
Extinction coefficient	230,120 (calc based on 88 Y, 18 W, and 0 C residues)
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</protect> Domains/Motifs/Modification Sites

<protect> See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	998-2103	mini RNAP
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<motif_map/>

Structure
<protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures	<beststructure> gene=Phage_N4_50 taxon=10752 </beststructure>
Models	View models at: * PSI-PRED * Genomes TO Protein (GTOP)
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Structure figures

See Help:Jmol in EcoliWiki
3C46:bacteriophage N4 mini-virion RNA polymerase
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >3C46.pdb</uploadedFileContents> <name>3C46</name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>

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Notes

The structure of the polymerase domain bound to the hairpin promoter <jmol> <jmolLink> <target>3C46</target> <script> select dna; spacefill on; rotate off; </script> <text>(highlight)</text> </jmolLink> </jmol>was described by Gleghorn et al.^[2].

Gene Product Resources

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<protect> See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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<protect> See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Notes

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_008720:45005..45045 source=bacteriophage_N4 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus. </protect>

Notes

Turnover and Regulation of Turnover

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Notes

Experimental

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<protect> Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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</protect> Expression Studies <protect> See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
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</protect> Expression Resources <protect> See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to Phage N4 50 Expression

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

*BLAST *CDD *InParanoid *YOGY <protect></protect>

Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 NCBI: Enterobacteria phage N4, complete genome http://www.ncbi.nlm.nih.gov/nuccore/NC_008720 Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content
↑ Gleghorn, ML et al. (2008) Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase. Mol. Cell 32 707-17 PubMed

Phage N4 50:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to Phage N4 50 Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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