Phage N4 50:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

50

Synonyms

gp50 [1], EPNV4_gp50 [1]

Product description

virion RNA polymerase [1]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003899

DNA-directed RNA polymerase activity

PMID:6989837[2]

IDA: Inferred from Direct Assay

F

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MSVFDRLAGF ADSVTNAKQV DVSTATAQKK AEQGVTTPLV SPDAAYQMQA ARTGNVGANA
FEPGTVQSDF MNLTPMQIMN KYGVEQGLQL INARADAGNQ VFNDSVTTRT PGEELGDIAT
GVGLGFVNTL GGIGALGAGL LNDDAGAVVA QQLSKFNDAV HATQSQALQD KRKLFAARNL
MNEVESERQY QTDKKEGTND IVASLSKFGR DFVGSIENAA QTDSIISDGL AEGVGSLLGA
GPVLRGASLL GKAVVPANTL RSAALAGAID AGTGTQSLAR IASTVGRAAP GMVGVGAMEA
GGAYQQTADE IMKMSLKDLE KSPVYQQHIK DGMSPEQARR QTASETGLTA AAIQLPIAAA
TGPLVSRFEM APFRAGSLGA VGMNLARETV EEGVQGATGQ LAQNIAQQQN IDKNQDLLKG
VGTQAGLGAL YGFGSAGVVQ APAGAARLAG AATAPVLRTT MAGVKAAGSV AGKVVSPIKN
TLVARGERVM KQNEEASPVA DDYVAQAAQE AMAQAPEAEV TIRDAVEATD ATPEQKVAAH
QYVSDLMNAT RFNPENYQEA PEHIRNAVAG STDQVQVIQK LADLVNTLDE SNPQALMEAA
SYMYDAVSEF EQFINRDPAA LDSIPKDSPA IELLNRYTNL TANIQNTPKV IGALNVINRM
INESAQNGSL NVTEESSPQE MQNVALAAEV APEKLNPESV NVVLKHAADG RIKLNNRQIA
ALQNAAAILK GAREYDAEAA RLGLRPQDIV SKQIKTDESR TQEGQYSALQ HANRIRSAYN
SGNFELASAY LNDFMQFAQH MQNKVGALNE HLVTGNADKN KSVHYQALTA DREWVRSRTG
LGVNPYDTKS VKFAQQVALE AKTVADIANA LASAYPELKV SHIKVTPLDS RLNAPAAEVV
KAFRQGNRDV ASSQPKADSV NQVKETPVTK QEPVTSTVQT KTPVSESVKT EPTTKESSPQ
AIKEPVNQSE KQDVNLTNED NIKQPTESVK ETETSTKEST VTEELKEGID AVYPSLVGTA
DSKAEGIKNY FKLSFTLPEE QKSRTVGSEA PLKDVAQALS SRARYELFTE KETANPAFNG
EVIKRYKELM EHGEGIADIL RSRLAKFLNT KDVGKRFAQG TEANRWVGGK LLNIVEQDGD
TFKYNEQLLQ TAVLAGLQWR LTATSNTAIK DAKDVAAITG IDQALLPEGL VEQFDTGMTL
TEAVSSLAQK IESYWGLSRN PNAPLGYTKG IPTAMAAEIL AAFVESTDVV ENIVDMSEID
PDNKKTIGLY TITELDSFDP INSFPTAIEE AVLVNPTEKM FFGDDIPPVA NTQLRNPAVR
NTPEQKAALK AEQATEFYVH TPMVQFYETL GKDRILELMG AGTLNKELLN DNHAKSLEGK
NRSVEDSYNQ LFSVIEQVRA QSEDISTVPI HYAYNMTRVG RMQMLGKYNP QSAKLVREAI
LPTKATLDLS NQNNEDFSAF QLGLAQALDI KVHTMTREVM SDELTKLLEG NLKPAIDMMV
EFNTTGSLPE NAVDVLNTAL GDRKSFVALM ALMEYSRYLV AEDKSAFVTP LYVEADGVTN
GPINAMMLMT GGLFTPDWIR NIAKGGLFIG SPNKTMNEHR STADNNDLYQ ASTNALMESL
GKLRSNYASN MPIQSQIDSL LSLMDLFLPD INLGENGALE LKRGIAKNPL TITIYGSGAR
GIAGKLVSSV TDAIYERMSD VLKARAKDPN ISAAMAMFGK QAASEAHAEE LLARFLKDME
TLTSTVPVKR KGVLELQSTG TGAKGKINPK TYTIKGEQLK ALQENMLHFF VEPLRNGITQ
TVGESLVYST EQLQKATQIQ SVVLEDMFKQ RVQEKLAEKA KDPTWKKGDF LTQKELNDIQ
ASLNNLAPMI ETGSQTFYIA GSENAEVANQ VLATNLDDRM RVPMSIYAPA QAGVAGIPFM
TIGTGDGMMM QTLSTMKGAP KNTLKIFDGM NIGLNDITDA SRKANEAVYT SWQGNPIKNV
YESYAKFMKN VDFSKLSPEA LEAIGKSALE YDQRENATVD DIANAASLIE RNLRNIALGV
DIRHKVLDKV NLSIDQMAAV GAPYQNNGKI DLSNMTPEQQ ADELNKLFRE ELEARKQKVA
KARAEVKEET VSEKEPVNPD FGMVGREHKA SGVRILSATA IRNLAKISNL PSTQAATLAE
IQKSLAAKDY KIIYGTPTQV AEYARQKNVT ELTSQEMEEA QAGNIYGWTN FDDKTIYLVS
PSMETLIHEL VHASTFEEVY SFYQGNEVSP TSKQAIENLE GLMEQFRSLD ISKDSPEMRE
AYADAIATIE GHLSNGFVDP AISKAAALNE FMAWGLANRA LAAKQKRTSS LVQMVKDVYQ
AIKKLIWGRK QAPALGEDMF SNLLFNSAIL MRSQPTTQAV AKDGTLFHSK AYGNNERLSQ
LNQTFDKLVT DYLRTDPVTE VERRGNVANA LMSATRLVRD VQSHGFNMTA QEQSVFQMVT
AALATEAAID PHAMARAQEL YTHVMKHLTV EHFMADPDST NPADRYYAQQ KYDTISGANL
VEVDAKGRTS LLPTFLGLAM VNEELRSIIK EMPVPKADKK LGNDIDTLLT NAGTQVMESL
NRRMAGDQKA TNVQDSIDAL SETIMAAALK RESFYDAVAT PTGNFIDRAN QYVTDSIERL
SETVIEKADK VIANPSNIAA KGVAHLAKLT AAIASEKQGE IVAQGVMTAM NQGKVWQPFH
DLVNDIVGRT KTNANVYDLI KLVKSQISQD RQQFREHLPT VIAGKFSRKL TDTEWSAMHT
GLGKTDLAVL RETMSMAEIR DLLSSSKKVK DEISTLEKEI QNQAGRNWNL VQKKSKQLAQ
YMIMGEVGNN LLRNAHAISR LLGERITNGP VADVAAIDKL ITLYSLELMN KSDRDLLSEL
AQSEVEGMEF SIAYMVGQRT EEMRKAKGDN RTLLNHFKGY IPVENQQGVN LIIADDKEFA
KLNSQSFTRI GTYQGSTGFR TGSKGYYFSP VAARAPYSQG ILQNVRNTAG GVDIGTGFTL
GTMVAGRITD KPTVERITKA LAKGERGREP LMPIYNSKGQ VVAYEQSVDP NMLKHLNQDN
HFAKMVGVWR GRQVEEAKAQ RFNDILIEQL HAMYEKDIKD SSANKSQYVN LLGKIDDPVL
ADAINLMNIE TRHKAEELFG KDELWVRRDM LNDALGYRAA SIGDVWTGNS RWSPSTLDTV
KKMFLGAFGN KAYHVVMNAE NTIQNLVKDA KTVIVVKSVV VPAVNFLANI YQMIGRGVPV
KDIAVNIPRK TSEINQYIKS RLRQIDAEAE LRAAEGNPNL VRKLKTEIQS ITDSHRRMSI
WPLIEAGEFS SIADAGISRD DLLVAEGKIH EYMEKLANKL PEKVRNAGRY ALIAKDTALF
QGIQKTVEYS DFIAKAIIYD DLVKRKKKSS SEALGQVTEE FINYDRLPGR
Length 3,410
Mol. Wt 372.322 kDa
pI 5.7 (calculated)
Extinction coefficient 230,120 (calc based on 88 Y, 18 W, and 0 C residues)
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</protect> Domains/Motifs/Modification Sites

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Type Residues Description Notes References
Domain 998-2103 mini RNAP
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Structure
<protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.
Structures <beststructure> gene=Phage_N4_50 taxon=10752 </beststructure>
Models View models at: * PSI-PRED * Genomes TO Protein (GTOP)
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Structure figures

See Help:Jmol in EcoliWiki
3C46:bacteriophage N4 mini-virion RNA polymerase
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >3C46.pdb</uploadedFileContents> <name>3C46</name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>
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Notes

The structure of the polymerase domain bound to the hairpin promoter <jmol> <jmolLink> <target>3C46</target> <script> select dna; spacefill on; rotate off; </script> <text>(highlight)</text> </jmolLink> </jmol>was described by Gleghorn et al.[3].

Gene Product Resources

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Resource type Source Notes/Reference
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Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 NCBI: Enterobacteria phage N4, complete genome http://www.ncbi.nlm.nih.gov/nuccore/NC_008720 Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content Cite error: Invalid <ref> tag; name "NC_008720" defined multiple times with different content
  2. ↑ Falco, SC et al. (1980) DNA-dependent RNA polymerase from bacteriophage N4 virions. Purification and characterization. J. Biol. Chem. 255 4339-47 PubMed
  3. ↑ Gleghorn, ML et al. (2008) Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase. Mol. Cell 32 707-17 PubMed

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