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Get StartedFind information within EcoliWiki by using the Search box to the left. Just enter your favorite E. coli gene or gene product. You can also browse our categories if you want to see what's here. Some sample queries are listed below, and you can always read more about searching on our Help pages.
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This page contains links to information about various E. coli Strains.
If you were looking for genes or a list of genomes in EcoliWiki, see Category:E._coli_genomes.
We've generated different sub-categories for easier browsing and individual strains are listed below the sub-categories. Please note that this list is not all-inclusive, but merely a starting point for some of the more commonly used strains. For help on strains, please see Help:Strains. To add a new strain, visit the Page_Generator#Strain_Page.
See also
- BCCM/GeneCorner Plasmid Collection host strains
- Coli Genetic Stock Center (CGSC)
- Strain information at OpenWetWare
- Open Biosystems Non-mammalian sets
See Help:Strains for more information about strains in EcoliWiki.
<protect>
| Name |
BW25113 |
|---|---|
| Synonyms | |
| Genotype |
F- DE(araD-araB)567 lacZ4787(del)::rrnB-3 LAM- rph-1 DE(rhaD-rhaB)568 hsdR514 |
| Phenotype | |
| Plasmids | |
| Phage | |
| Sources | |
| Construction |
see description below |
| References | |
</protect>
Notes
BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] BW25113 is available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2].
According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
BW25113 is a derivative of E. coli K-12 strain BD792, which has the genotype F- LAM- rpoS396(Am) rph-1.
The steps in the construction of BW25113 are described below. This information is from Supplementary Table 1 of Baba et al.([2]). At several steps of the strain construction, mutant alleles were constructed in vitro and recombined into the genome using oriRR6Kgamma plasmids, which cannot replicate in a strain that lacks the R6K pir gene ([3]). In the absence of selection of the antibiotic resistance marker carried on the plasmid, homologous recombination can occur that will either restore the original genotype or result in allele replacement.
| Step | Strain | Genotype | Construction | Reference |
|---|---|---|---|---|
|
ii) |
BW21597 |
F- LAM- rpoS396(Am) DEphoR574 rph-1 |
BD792 was made TcR with pAH14 then TcS Pho- derivatives were isolated.[4] |
unpublished |
|
iii) |
BW21652 |
F- LAM- rpoS396(Am) DEphoR574 DE(creABCD)154 rph-1 |
BW21597 was made TcR with pSK26 and then TcS Cre- derivatives were isolated. [3] |
unpublished |
|
iv) |
BW23186 |
F- LAM- rpoS396(Am) DEphoR574 DE(creABCD)154 DE(araBAD)567 rph-1 |
BW21652 was made TcR with pAH54 and then TcS Ara- derivatives were isolated. ([5]) |
unpublished |
|
v) |
BW23599 |
F- LAM- rpoS396(Am) rrnB3 DElacZ4787 proC::Tn5-132 DE(creABCD)154 DE(araBAD)567 rph-1 |
BW23186 was transduced to TcR Pro- Lac- Pho+ with P1kc grown on BW22773[5] |
unpublished |
|
vi) |
BW23660 |
F- LAM- rpoS396(Am) rrnB3 DElacZ4787 DE(phoBR)580 DE(creABCD)154 DE(araBAD)567 rph-1 |
BW23599 was transduced to Pro+ Pho- with P1kc grown on BW23316. |
Haldimann et al. 1997[4] |
|
vii) |
BW24200 |
F- LAM- rpoS396(Am) rrnB3 DElacZ4787 DE(phoBR)580 DE(creABCD)154 DE(araBAD)567 metF159(Am) zii-510::Tn10 rph-1 |
BW23660 was transduced to TcR Met- with P1kc grown on JW383 (Haldimann et al., 1997) |
unpublished |
|
viii) |
BW24201 |
F- LAM- rpoS396(Am) rrnB3 DElacZ4787 DE(phoBR)580 DE(creABCD)154 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
BW24200 was transduced to Met+ TcS Rha- with P1kc grown on BW22875[5] |
unpublished |
|
ix) |
BW24310 |
F- LAM- rrnB3 DElacZ4787 DE(phoBR)580 DE(creABCD)154 DE(araBAD)567 DE(rhaBAD)568 cysC95::Tn10 rpoS::kan rph-1 |
BW24201 was transduced to TcR KmR with P1kc grown on ZK1001[5] |
Haldimann and Wanner, 2001[6] |
|
x) |
BW24320a |
F- LAM- rrnB3 DElacZ4787 DE(phoBR)580 DE(creABCD)154 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
BW24310 was transduced to Cys+ KmS with P1kc grown on MG1655 (Wanner and Boline, 1990) aBW24320 and its descendants carry the same pseudorevertant rpoS(Q33) allele as MG1655 (Hayashi et al., 2005) |
Haldimann and Wanner, 2001[6] |
|
xi) |
BW24353 |
F- LAM- rrnB3 DElacZ4787 DE(phoBR)580 DE(zaa trpR creABCD arcA thr)104::Tn5-132 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
BW24320 was transduced to TcR Thr- with P1kc grown on BW12070 (Fisher et al., 1995) |
unpublished |
|
xii) |
BW24476 |
F- LAM- rrnB3 DElacZ4787 DE(phoBR)580 hsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
BW24353 was transduced to Thr+ HsdR- with P1kc grown on BW24447[7] |
Stern et al., 1999[7] |
|
xiii) |
BW25083 |
F- LAM- rrnB3 DElacZ4787 proC::Tn5-132 hsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
BW24476 was transduced to TcR Pro- Pho+ with P1kc grown on BW22773[5] |
Haldimann and Wanner, 2001[6] |
|
xiv) |
BW25113 |
F- LAM- rrnB3 DElacZ4787 hsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
BW25083 was transduced to Pro+ with P1kc grown on BW24321[6] |
|
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
- ↑ 2.0 2.1 2.2 2.3 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 Metcalf, WW et al. (1996) Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35 1-13 PubMed
- ↑ 4.0 4.1 Haldimann, A et al. (1997) Transcriptional regulation of the Enterococcus faecium BM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system in Escherichia coli K-12. J. Bacteriol. 179 5903-13 PubMed
- ↑ 5.0 5.1 5.2 5.3 5.4 Haldimann, A et al. (1998) Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J. Bacteriol. 180 1277-86 PubMed
- ↑ 6.0 6.1 6.2 6.3 Haldimann, A & Wanner, BL (2001) Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183 6384-93 PubMed
- ↑ 7.0 7.1 Stern, RJ et al. (1999) Conversion of dTDP-4-keto-6-deoxyglucose to free dTDP-4-keto-rhamnose by the rmIC gene products of Escherichia coli and Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 145 ( Pt 3) 663-71 PubMed
- ↑ Lessard, IA et al. (1998) Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function. Chem. Biol. 5 489-504 PubMed
