tyrA:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tyrA

Mnemonic
Synonyms

ECK2597, b2600, JW2581[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

58.99 minutes, 58.99 minutes 

MG1655: 2738091..2736970
gbrowse_img.php?coord=2735970..2739091&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2738725..2737604
gbrowse_img.php?coord=2736604..2739725&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2829856..2828735
gbrowse_img.php?coord=2826493..2832098&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtyrA (Keio:JW2581)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10049[3]

tyrA::Tn5KAN

PMID:12519969[4]

ASAP

At position 491 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20865

tyrA4

CGSC:4851

tyrA2

CGSC:5299

tyrA14

CGSC:7359

tyrA15(Am)

CGSC:8790

amber (UAG) mutation

tyrA16::Tn10

CGSC:8868

tyrA6

CGSC:8934

tyrA1::Mu

CGSC:23654

tyrA117::Mu cts

CGSC:73503

ΔtyrA763::kan

PMID:16738554[2]

CGSC:103260


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2581

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGTTGCTGAATTGACCGCATT

Primer 2:CCtTGGCGATTGTCATTCGCCTG

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[3]

est. P1 cotransduction: 35% [6]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[3]

est. P1 cotransduction: 99% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11039

EchoBASE (EcoliWiki Page)

EchoBASE:EB1032

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2600

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3121

EcoGene (EcoliWiki Page)

EcoGene:EG11039

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TYRA

CGSC (EcoliWiki Page)

CGSC:58

ASAP (EcoliWikiPage)

ASAP:ABE-0008547


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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