argA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argA

Mnemonic

Arginine

Synonyms

ECK2814, b2818, JW2786, Arg1, Arg2[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.52 minutes 

MG1655: 2947264..2948595
gbrowse_img.php?coord=2947264..2948595&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2843861..2845192
gbrowse_img.php?coord=2843861..2845192&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2834412..2835743
gbrowse_img.php?coord=2834412..2835743&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2947898..2949229
gbrowse_img.php?coord=2947898..2949229&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3041134..3042465
gbrowse_img.php?coord=3041134..3042465&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2947267

Edman degradation

PMID:3320971[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

argAR58H

R58H

In EE11

seeded from UniProt:P0A6C5

argAH15Y

H15Y

In EE17

seeded from UniProt:P0A6C5

argAY19C

Y19C

In EE51

seeded from UniProt:P0A6C5

argAG287S

G287S

In PT2M216

seeded from UniProt:P0A6C5

argAQ432R

Q432R

In PT2M217

seeded from UniProt:P0A6C5

argAS54N

S54N

In PT2M217

seeded from UniProt:P0A6C5

ΔargA (Keio:JW2786)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10181[4]

argA::Tn5KAN

PMID:12519969[5]

ASAP

At position 933 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20943

argA::Tn5KAN

PMID:12519969[5]

ASAP

At position 933 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20944

argA21

CGSC:5137

argA42

CGSC:5157

argA52

CGSC:5167

argA76(Fs)

CGSC:5192

frameshift mutation

argA103

CGSC:5207

argA22

CGSC:6000

argA81::Tn10

CGSC:7098

argA9999

CGSC:9194

ΔargA743::kan

PMID:16738554[3]

CGSC:100036



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2786

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGTAAAGGAACGTAAAACCGA

Primer 2:CCgCCTAAATCCGCCATCAACAC

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[4]

est. P1 cotransduction: 59% [7]
Synonyms:fuc-3072::Tn10

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[4]

est. P1 cotransduction: 89% [7]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10063

EchoBASE (EcoliWiki Page)

EchoBASE:EB0061

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2818

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3412

EcoGene (EcoliWiki Page)

EcoGene:EG10063

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ARGA

CGSC (EcoliWiki Page)

CGSC:1021

ASAP (EcoliWikiPage)

ASAP:ABE-0009245



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Brown, K et al. (1987) Complete nucleotide sequence of the Escherichia coli argA gene. Nucleic Acids Res. 15 10586 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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