prmB:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

prmB

Mnemonic

Protein methylation

Synonyms

ECK2324, b2330, JW5841, yfcB[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

52.71 minutes 

MG1655: 2446462..2445530
gbrowse_img.php?coord=2445530..2446462&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2391100..2392032
gbrowse_img.php?coord=2391100..2392032&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2453886..2452954
gbrowse_img.php?coord=2452954..2453886&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2538227..2537295
gbrowse_img.php?coord=2537295..2538227&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔprmB (Keio:JW5841)

deletion

deletion

PMID:16738554[2]

Shigen

yfcB::Tn5KAN

PMID:12519969[3]

ASAP

At position 691 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20768

yfcB::Tn5KAN

PMID:12519969[3]

ASAP

At position 691 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20769


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5841

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGATAAAATTTTCGTTGATGA

Primer 2:CCATCTTTATAAATCGCGAAATG

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[5]

est. P1 cotransduction: 6% [6]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[5]

est. P1 cotransduction: 13% [6]
Synonyms:zfb-1::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12449

EchoBASE (EcoliWiki Page)

EchoBASE:EB2343

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2330

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2876

EcoGene (EcoliWiki Page)

EcoGene:EG12449

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YFCB

ASAP (EcoliWikiPage)

ASAP:ABE-0007695



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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