prmB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PrmB

Synonyms

N5-glutamine methyltransferase[1], PrmB[2][1], YfcB[2][1], B2330[2][1] , ECK2324, JW5841, yfcB, b2330

Product description

EG12449[2][3]

50S subunit L3 protein glutamine methyltransferase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002052

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017127

C

Seeded from EcoCyc (v14.0)

complete

GO:0006306

DNA methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017127

P

Seeded from EcoCyc (v14.0)

complete

GO:0006479

protein amino acid methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004556

P

Seeded from EcoCyc (v14.0)

complete

GO:0008168

methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002052

F

Seeded from EcoCyc (v14.0)

complete

GO:0008168

methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007848

F

Seeded from EcoCyc (v14.0)

complete

GO:0008168

methyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0489

F

Seeded from EcoCyc (v14.0)

complete

GO:0008276

protein methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004556

F

Seeded from EcoCyc (v14.0)

complete

GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017127

F

Seeded from EcoCyc (v14.0)

complete

GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.1.1.72

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0032259

methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002052

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsJ

PMID:19402753[5]

LCMS(ID Probability):99.6

Protein

tig

PMID:19402753[5]

LCMS(ID Probability):99.4 MALDI(Z-score):26.555363

Protein

mcbR

PMID:19402753[5]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAGISLLLDQ AFHRHFADFF TLRSVDNRVL LNLASPRHQL AKQSLVLLAK CCFLFHLSVS
ISNFPTVYPT RSAIDRFKRI FPHSPRVNAD LSSSSWQTSR RNYCDHALEE YVDKIFVDEA
VNELQTIQDM LRWSVSRFSA ANIWYGHGTD NPWDEAVQLV LPSLYLPLDI PEDMRTARLT
SSEKHRIVER VIRRVNERIP VAYLTNKAWF CGHEFYVDER VLVPRSPIGE LINNKFAGLI
SKQPQHILDM CTGSGCIAIA CAYAFPDAEV DAVDISPDAL AVAEQNIEEH GLIHNVIPIR
SDLFRDLPKV QYDLIVTNPP YVDAEDMSDL PNEYRHEPEL GLASGTDGLK LTRRILGNAA
DYLADDGVLI CEVGNSMVHL MEQYPDVPFT WLEFDNGGDG VFMLTKEQLI AAREHFAIYK D
Length

421

Mol. Wt

47.762 kDa

pI

5.2 (calculated)

Extinction coefficient

53,860 - 54,860 (calc based on 14 Y, 6 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

124..273

PF05175 Methyltransferase small domain

PMID:19920124[6]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=prmB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111419

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946805

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007695

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P39199

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12449

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12449

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946805

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002301

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2343

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  6. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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