dnaK:Gene Product(s)

Standard name	DnaK
Synonyms	chaperone Hsp70, co-chaperone with DnaJ^[1], B0014^[2]^[1], GrpC^[2]^[1], GrpF^[2]^[1], Seg^[2]^[1], GroP^[2]^[1], DnaL^[2]^[1], DnaK^[2]^[1]
Product description	chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins^[2]^[3]; Component of DnaJ/DnaK/GrpE^[2]^[3] Hsp70 molecular chaperone, heat-inducible^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0051082	unfolded protein binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	F	Seeded from EcoCyc ^[5]	complete
	GO:0016020	membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc ^[5]	complete
	GO:0006950	response to stress	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P	Seeded from EcoCyc ^[5]	complete
	GO:0006457	protein folding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	P	Seeded from EcoCyc ^[5]	complete
	GO:0006260	DNA replication	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P	Seeded from EcoCyc ^[5]	complete
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005524	ATP binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR001023 InterPro:IPR012725 SP_KW:KW-0067 HAMAP:MF_00332	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc ^[5]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0A853	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0AAI5	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0AB71	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC38	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC81	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0ACX3	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P0ADX9	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P12282	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P23721	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P28304	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P37342	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P45578	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:15690043^[7]	IPI: Inferred from Physical Interaction	UniProtKB:P69924	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16139413^[8]	IPI: Inferred from Physical Interaction	UniProtKB:P21513	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P00634	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P00811	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A6Z3	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9Q9	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0AG80	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0C0V0	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P10346	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P16700	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P17117	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P28635	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P30011	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P36677	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P37024	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P37685	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P46853	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P62623	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P68919	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P69822	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P76002	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:Q46796	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:Q46906	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005737	cytoplasm	PMID:16858726^[10]	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005886	plasma membrane	GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc ^[5]	complete
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc	complete
	GO:0005515	protein binding	PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:15690043^[7] PMID:16139413^[8] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9] PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A853 UniProtKB:P0AAI5 UniProtKB:P0AB71 UniProtKB:P0AC38 UniProtKB:P0AC81 UniProtKB:P0ACX3 UniProtKB:P0ADX9 UniProtKB:P12282 UniProtKB:P23721 UniProtKB:P28304 UniProtKB:P37342 UniProtKB:P45578 UniProtKB:P69924 UniProtKB:P21513 UniProtKB:P00634 UniProtKB:P00811 UniProtKB:P0A6Z3 UniProtKB:P0A9Q9 UniProtKB:P0AG80 UniProtKB:P0C0V0 UniProtKB:P10346 UniProtKB:P16700 UniProtKB:P17117 UniProtKB:P28635 UniProtKB:P30011 UniProtKB:P36677 UniProtKB:P37024 UniProtKB:P37685 UniProtKB:P46853 UniProtKB:P62623 UniProtKB:P68919 UniProtKB:P69822 UniProtKB:P76002 UniProtKB:Q46796 UniProtKB:Q46906	F	Seeded from EcoCyc	complete
	GO:0005524	ATP binding	GO_REF:0000020 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	HAMAP:MF_00332 InterPro:IPR001023 InterPro:IPR012725 SP_KW:KW-0067	F	Seeded from EcoCyc	complete
	GO:0005737	cytoplasm	GO_REF:0000004 GO_REF:0000023 PMID:16858726^[10]	IDA: Inferred from Direct Assay	SP_KW:KW-0963 SP_SL:SL-0086	C	Seeded from EcoCyc	complete
	GO:0005886	plasma membrane	GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc	complete
	GO:0006970	response to osmotic stress				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0000910	cytokinesis				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0006260	DNA replication	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P	Seeded from EcoCyc	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of DnaJ/DnaK/GrpE	could be indirect
Protein	htpG		PMID:16606699^[9]	Experiment(s):EBI-1135316
Protein	aphA		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	gadA		PMID:15690043^[7]	Experiment(s):EBI-883577, EBI-888455, EBI-893820
Protein	grpE		PMID:15690043^[7]	Experiment(s):EBI-883577, EBI-881166, EBI-888219, EBI-890853
Protein	lon		PMID:15690043^[7]	Experiment(s):EBI-883577
Protein	rplF		PMID:15690043^[7]	Experiment(s):EBI-883577
Protein	rplJ		PMID:15690043^[7]	Experiment(s):EBI-883577
Protein	rpsB		PMID:15690043^[7]	Experiment(s):EBI-883577, EBI-890853
Protein	tufA		PMID:15690043^[7]	Experiment(s):EBI-883577, EBI-894882, EBI-890853
Protein	yibA		PMID:15690043^[7]	Experiment(s):EBI-883577
Protein	ydcR		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	ydfJ		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	entB		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	fabB		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	hisC		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	hscA		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	narQ		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	pepB		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	proS		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	rplI		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	rplS		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	rpmG		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	sapA		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	tdcG		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	dcuD		PMID:15690043^[7]	Experiment(s):EBI-890853
Protein	yihM		PMID:15690043^[7]	Experiment(s):EBI-890853
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA DGILHVSAKD KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD HLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK
Length	638
Mol. Wt	69.11 kDa (calc) ^[2]
pI
Extinction coefficient	15930 - 16055 (calc based on 7 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes
Modification Site	199	Phosphothreonine; by autocatalysis	UniProt:P0A6Y8
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<motif_map/>

Structure See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

Models

View models at:

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Notes

Like other Hsp70 chaperones, dnaK consists of two domains: and N-terminal ATPase domain, a substrate binding domain, and a short DnaJ binding domain^[11]. Structures

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10241-MONOMER
SwissModel (EcoliWiki Page)	SwissModel:P0A6Y8
UniProt (EcoliWiki Page)	UniProt:P0A6Y8
PFAM (EcoliWiki Page)	PFAM:PF00012
RefSeq (EcoliWiki Page)	RefSeq:NP_414555
PDB (EcoliWiki Page)	PDB:2BPR
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Notes

Links

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Name	URL	Comments
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References

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↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 ^5.6 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^6.00 ^6.01 ^6.02 ^6.03 ^6.04 ^6.05 ^6.06 ^6.07 ^6.08 ^6.09 ^6.10 ^6.11 ^6.12 ^6.13 ^6.14 ^6.15 ^6.16 ^6.17 ^6.18 ^6.19 ^6.20 ^6.21 ^6.22 ^6.23 ^6.24 ^6.25 ^6.26 ^6.27 ^6.28 ^6.29 ^6.30 ^6.31 ^6.32 ^6.33 ^6.34 ^6.35 ^6.36 ^6.37 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^7.00 ^7.01 ^7.02 ^7.03 ^7.04 ^7.05 ^7.06 ^7.07 ^7.08 ^7.09 ^7.10 ^7.11 ^7.12 ^7.13 ^7.14 ^7.15 ^7.16 ^7.17 ^7.18 ^7.19 ^7.20 ^7.21 ^7.22 ^7.23 ^7.24 ^7.25 ^7.26 ^7.27 ^7.28 ^7.29 ^7.30 ^7.31 ^7.32 ^7.33 ^7.34 ^7.35 ^7.36 ^7.37 ^7.38 ^7.39 ^7.40 ^7.41 ^7.42 ^7.43 ^7.44 ^7.45 ^7.46 ^7.47 ^7.48 ^7.49 ^7.50 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
↑ ^8.0 ^8.1 Regonesi, ME et al. (2006) Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach. Biochimie 88 151-61 PubMed
↑ ^9.00 ^9.01 ^9.02 ^9.03 ^9.04 ^9.05 ^9.06 ^9.07 ^9.08 ^9.09 ^9.10 ^9.11 ^9.12 ^9.13 ^9.14 ^9.15 ^9.16 ^9.17 ^9.18 ^9.19 ^9.20 ^9.21 ^9.22 ^9.23 ^9.24 ^9.25 ^9.26 ^9.27 ^9.28 ^9.29 ^9.30 ^9.31 ^9.32 ^9.33 ^9.34 ^9.35 ^9.36 ^9.37 ^9.38 ^9.39 ^9.40 ^9.41 ^9.42 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ ^10.0 ^10.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
↑ Genevaux, P et al. (2007) The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol. Microbiol. 66 840-57 PubMed

dnaK:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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dnaK:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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